Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20215 and BWI76_RS20220 are separated by 76 nucleotides BWI76_RS20220 and BWI76_RS20225 are separated by 11 nucleotides BWI76_RS20225 and BWI76_RS20230 are separated by 86 nucleotides BWI76_RS20230 and BWI76_RS20235 are separated by 336 nucleotides
BWI76_RS20215: BWI76_RS20215 - transcriptional regulator, at 4,236,424 to 4,238,256
_RS20215
BWI76_RS20220: BWI76_RS20220 - sugar phosphatase, at 4,238,333 to 4,238,992
_RS20220
BWI76_RS20225: BWI76_RS20225 - hypothetical protein, at 4,239,004 to 4,239,498
_RS20225
BWI76_RS20230: BWI76_RS20230 - hypothetical protein, at 4,239,585 to 4,240,040
_RS20230
BWI76_RS20235: BWI76_RS20235 - acetate kinase, at 4,240,377 to 4,241,579
_RS20235
Position (kb) 4239
4240 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 4238.082 kb on + strand at 4238.124 kb on + strand at 4238.156 kb on + strand at 4238.231 kb on - strand at 4238.251 kb on + strand at 4238.558 kb on - strand, within BWI76_RS20220 at 4238.720 kb on - strand, within BWI76_RS20220 at 4238.738 kb on - strand, within BWI76_RS20220 at 4238.979 kb on + strand at 4238.979 kb on + strand at 4238.979 kb on + strand at 4238.979 kb on + strand at 4238.987 kb on - strand at 4239.045 kb on - strand at 4239.045 kb on - strand at 4239.226 kb on + strand, within BWI76_RS20225 at 4239.369 kb on + strand, within BWI76_RS20225 at 4239.469 kb on + strand at 4239.516 kb on + strand at 4239.637 kb on - strand, within BWI76_RS20230 at 4239.643 kb on + strand, within BWI76_RS20230 at 4240.035 kb on + strand at 4240.164 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 4,238,082 + -2.6 4,238,124 + +1.2 4,238,156 + +0.3 4,238,231 - -0.1 4,238,251 + -0.1 4,238,558 - BWI76_RS20220 0.34 +1.3 4,238,720 - BWI76_RS20220 0.59 -0.1 4,238,738 - BWI76_RS20220 0.61 +0.0 4,238,979 + -0.2 4,238,979 + -0.1 4,238,979 + +0.9 4,238,979 + -0.4 4,238,987 - +0.6 4,239,045 - +0.2 4,239,045 - -0.5 4,239,226 + BWI76_RS20225 0.45 +0.3 4,239,369 + BWI76_RS20225 0.74 +4.3 4,239,469 + -0.0 4,239,516 + -0.2 4,239,637 - BWI76_RS20230 0.11 +0.2 4,239,643 + BWI76_RS20230 0.13 -0.2 4,240,035 + -0.1 4,240,164 - -0.9
Or see this region's nucleotide sequence