Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17525 and BWI76_RS17530 overlap by 96 nucleotides BWI76_RS17530 and BWI76_RS17535 are separated by 251 nucleotides BWI76_RS17535 and BWI76_RS17540 overlap by 4 nucleotides
BWI76_RS17525: BWI76_RS17525 - XshC-Cox1-family protein, at 3,634,823 to 3,635,656
_RS17525
BWI76_RS17530: BWI76_RS17530 - hypothetical protein, at 3,635,561 to 3,635,938
_RS17530
BWI76_RS17535: BWI76_RS17535 - rRNA metabolism protein, at 3,636,190 to 3,639,294
_RS17535
BWI76_RS17540: BWI76_RS17540 - chlorohydrolase/aminohydrolase, at 3,639,291 to 3,640,628
_RS17540
Position (kb)
3636
3637
3638
3639
3640 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 3635.433 kb on - strand, within BWI76_RS17525 at 3635.549 kb on + strand, within BWI76_RS17525 at 3635.563 kb on + strand at 3635.571 kb on - strand at 3635.582 kb on + strand at 3635.590 kb on - strand at 3635.603 kb on + strand at 3635.613 kb on - strand at 3635.615 kb on + strand at 3635.623 kb on - strand at 3635.623 kb on - strand at 3635.623 kb on - strand at 3635.623 kb on - strand at 3635.631 kb on - strand at 3635.670 kb on - strand, within BWI76_RS17530 at 3635.671 kb on - strand, within BWI76_RS17530 at 3635.685 kb on + strand, within BWI76_RS17530 at 3635.765 kb on + strand, within BWI76_RS17530 at 3635.853 kb on + strand, within BWI76_RS17530 at 3635.884 kb on + strand, within BWI76_RS17530 at 3635.974 kb on + strand at 3635.982 kb on - strand at 3635.982 kb on - strand at 3636.000 kb on + strand at 3636.051 kb on + strand at 3636.059 kb on - strand at 3636.133 kb on + strand at 3636.147 kb on - strand at 3636.173 kb on + strand at 3636.175 kb on + strand at 3636.186 kb on + strand at 3636.249 kb on + strand at 3636.249 kb on + strand at 3636.249 kb on + strand at 3636.257 kb on - strand at 3636.331 kb on + strand at 3636.344 kb on - strand at 3636.349 kb on - strand at 3636.393 kb on - strand at 3636.432 kb on + strand at 3636.440 kb on - strand at 3636.474 kb on + strand at 3636.501 kb on + strand, within BWI76_RS17535 at 3636.555 kb on + strand, within BWI76_RS17535 at 3636.563 kb on - strand, within BWI76_RS17535 at 3636.587 kb on + strand, within BWI76_RS17535 at 3636.599 kb on - strand, within BWI76_RS17535 at 3636.599 kb on - strand, within BWI76_RS17535 at 3636.665 kb on + strand, within BWI76_RS17535 at 3636.665 kb on + strand, within BWI76_RS17535 at 3636.713 kb on + strand, within BWI76_RS17535 at 3636.747 kb on - strand, within BWI76_RS17535 at 3636.832 kb on - strand, within BWI76_RS17535 at 3636.852 kb on + strand, within BWI76_RS17535 at 3636.860 kb on - strand, within BWI76_RS17535 at 3636.970 kb on + strand, within BWI76_RS17535 at 3637.008 kb on - strand, within BWI76_RS17535 at 3637.234 kb on - strand, within BWI76_RS17535 at 3637.339 kb on - strand, within BWI76_RS17535 at 3637.557 kb on + strand, within BWI76_RS17535 at 3637.717 kb on + strand, within BWI76_RS17535 at 3638.062 kb on + strand, within BWI76_RS17535 at 3638.070 kb on - strand, within BWI76_RS17535 at 3638.141 kb on + strand, within BWI76_RS17535 at 3638.156 kb on + strand, within BWI76_RS17535 at 3638.198 kb on + strand, within BWI76_RS17535 at 3638.330 kb on + strand, within BWI76_RS17535 at 3638.444 kb on + strand, within BWI76_RS17535 at 3638.627 kb on - strand, within BWI76_RS17535 at 3638.721 kb on + strand, within BWI76_RS17535 at 3638.901 kb on - strand, within BWI76_RS17535 at 3639.033 kb on + strand at 3639.266 kb on + strand at 3639.405 kb on - strand at 3639.443 kb on - strand, within BWI76_RS17540 at 3639.611 kb on - strand, within BWI76_RS17540 at 3639.708 kb on - strand, within BWI76_RS17540 at 3639.871 kb on - strand at 3639.929 kb on - strand, within BWI76_RS17540 at 3639.940 kb on - strand, within BWI76_RS17540 at 3639.999 kb on + strand, within BWI76_RS17540 at 3640.092 kb on + strand, within BWI76_RS17540 at 3640.121 kb on + strand, within BWI76_RS17540 at 3640.172 kb on - strand, within BWI76_RS17540 at 3640.267 kb on - strand, within BWI76_RS17540
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 3,635,433 - BWI76_RS17525 0.73 +1.5 3,635,549 + BWI76_RS17525 0.87 -0.6 3,635,563 + +0.0 3,635,571 - +0.2 3,635,582 + +0.1 3,635,590 - +0.3 3,635,603 + +0.8 3,635,613 - +0.1 3,635,615 + -0.5 3,635,623 - +1.1 3,635,623 - -0.6 3,635,623 - +1.2 3,635,623 - +0.5 3,635,631 - -0.3 3,635,670 - BWI76_RS17530 0.29 -0.7 3,635,671 - BWI76_RS17530 0.29 +1.4 3,635,685 + BWI76_RS17530 0.33 +1.2 3,635,765 + BWI76_RS17530 0.54 -0.1 3,635,853 + BWI76_RS17530 0.77 +0.8 3,635,884 + BWI76_RS17530 0.85 -1.7 3,635,974 + +0.2 3,635,982 - +0.5 3,635,982 - -1.1 3,636,000 + +0.2 3,636,051 + +0.5 3,636,059 - -0.9 3,636,133 + +3.6 3,636,147 - +0.6 3,636,173 + +0.7 3,636,175 + +1.7 3,636,186 + +0.0 3,636,249 + -0.1 3,636,249 + -0.2 3,636,249 + -0.6 3,636,257 - +0.4 3,636,331 + +1.0 3,636,344 - +1.9 3,636,349 - +0.2 3,636,393 - +0.5 3,636,432 + -0.4 3,636,440 - +2.2 3,636,474 + +0.3 3,636,501 + BWI76_RS17535 0.10 +0.2 3,636,555 + BWI76_RS17535 0.12 +1.5 3,636,563 - BWI76_RS17535 0.12 -0.9 3,636,587 + BWI76_RS17535 0.13 -0.2 3,636,599 - BWI76_RS17535 0.13 -0.2 3,636,599 - BWI76_RS17535 0.13 +0.3 3,636,665 + BWI76_RS17535 0.15 -0.3 3,636,665 + BWI76_RS17535 0.15 +0.3 3,636,713 + BWI76_RS17535 0.17 +1.0 3,636,747 - BWI76_RS17535 0.18 -0.2 3,636,832 - BWI76_RS17535 0.21 -0.3 3,636,852 + BWI76_RS17535 0.21 +0.1 3,636,860 - BWI76_RS17535 0.22 +0.6 3,636,970 + BWI76_RS17535 0.25 +0.0 3,637,008 - BWI76_RS17535 0.26 +1.3 3,637,234 - BWI76_RS17535 0.34 +0.2 3,637,339 - BWI76_RS17535 0.37 -0.5 3,637,557 + BWI76_RS17535 0.44 +0.2 3,637,717 + BWI76_RS17535 0.49 +4.9 3,638,062 + BWI76_RS17535 0.60 +0.2 3,638,070 - BWI76_RS17535 0.61 +1.0 3,638,141 + BWI76_RS17535 0.63 +0.2 3,638,156 + BWI76_RS17535 0.63 -0.9 3,638,198 + BWI76_RS17535 0.65 +0.1 3,638,330 + BWI76_RS17535 0.69 -0.6 3,638,444 + BWI76_RS17535 0.73 -1.8 3,638,627 - BWI76_RS17535 0.78 +0.3 3,638,721 + BWI76_RS17535 0.82 -0.3 3,638,901 - BWI76_RS17535 0.87 +1.5 3,639,033 + +0.1 3,639,266 + +0.5 3,639,405 - -0.0 3,639,443 - BWI76_RS17540 0.11 -0.1 3,639,611 - BWI76_RS17540 0.24 -0.3 3,639,708 - BWI76_RS17540 0.31 -0.9 3,639,871 - +3.0 3,639,929 - BWI76_RS17540 0.48 +0.4 3,639,940 - BWI76_RS17540 0.49 +0.3 3,639,999 + BWI76_RS17540 0.53 +0.0 3,640,092 + BWI76_RS17540 0.60 +0.3 3,640,121 + BWI76_RS17540 0.62 -0.2 3,640,172 - BWI76_RS17540 0.66 -0.6 3,640,267 - BWI76_RS17540 0.73 -0.9
Or see this region's nucleotide sequence