Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15290

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15285 and BWI76_RS15290 are separated by 261 nucleotidesBWI76_RS15290 and BWI76_RS15295 are separated by 17 nucleotidesBWI76_RS15295 and BWI76_RS15300 are separated by 30 nucleotidesBWI76_RS15300 and BWI76_RS15305 are separated by 31 nucleotides BWI76_RS15285: BWI76_RS15285 - stress protection protein MarC, at 3,203,307 to 3,203,975 _RS15285 BWI76_RS15290: BWI76_RS15290 - transcriptional regulator, at 3,204,237 to 3,204,671 _RS15290 BWI76_RS15295: BWI76_RS15295 - MDR efflux pump AcrAB transcriptional activator MarA, at 3,204,689 to 3,205,069 _RS15295 BWI76_RS15300: BWI76_RS15300 - multiple antibiotic resistance regulatory periplasmic protein MarB, at 3,205,100 to 3,205,318 _RS15300 BWI76_RS15305: BWI76_RS15305 - O-acetylserine/cysteine exporter, at 3,205,350 to 3,206,249 _RS15305 Position (kb) 3204 3205Strain fitness (log2 ratio) -1 0 1at 3203.446 kb on - strand, within BWI76_RS15285at 3203.473 kb on - strand, within BWI76_RS15285at 3203.478 kb on - strand, within BWI76_RS15285at 3203.863 kb on + strand, within BWI76_RS15285at 3203.942 kb on + strandat 3203.950 kb on - strandat 3204.060 kb on - strandat 3204.104 kb on + strandat 3204.104 kb on + strandat 3204.122 kb on - strandat 3204.393 kb on + strand, within BWI76_RS15290at 3204.632 kb on + strandat 3204.875 kb on - strand, within BWI76_RS15295at 3204.875 kb on - strand, within BWI76_RS15295at 3204.917 kb on + strand, within BWI76_RS15295at 3204.918 kb on + strand, within BWI76_RS15295at 3204.926 kb on - strand, within BWI76_RS15295at 3205.029 kb on - strand, within BWI76_RS15295at 3205.150 kb on - strand, within BWI76_RS15300at 3205.156 kb on - strand, within BWI76_RS15300at 3205.163 kb on + strand, within BWI76_RS15300at 3205.171 kb on - strand, within BWI76_RS15300at 3205.256 kb on + strand, within BWI76_RS15300at 3205.296 kb on + strand, within BWI76_RS15300at 3205.560 kb on - strand, within BWI76_RS15305

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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3,203,446 - BWI76_RS15285 0.21 +0.3
3,203,473 - BWI76_RS15285 0.25 -0.6
3,203,478 - BWI76_RS15285 0.26 +0.5
3,203,863 + BWI76_RS15285 0.83 +0.0
3,203,942 + -0.5
3,203,950 - -0.3
3,204,060 - +0.2
3,204,104 + -0.3
3,204,104 + -0.9
3,204,122 - +1.3
3,204,393 + BWI76_RS15290 0.36 -1.3
3,204,632 + +0.2
3,204,875 - BWI76_RS15295 0.49 -0.1
3,204,875 - BWI76_RS15295 0.49 -0.1
3,204,917 + BWI76_RS15295 0.60 +0.3
3,204,918 + BWI76_RS15295 0.60 +0.7
3,204,926 - BWI76_RS15295 0.62 +0.0
3,205,029 - BWI76_RS15295 0.89 -0.3
3,205,150 - BWI76_RS15300 0.23 -0.5
3,205,156 - BWI76_RS15300 0.26 -0.7
3,205,163 + BWI76_RS15300 0.29 -0.1
3,205,171 - BWI76_RS15300 0.32 +1.1
3,205,256 + BWI76_RS15300 0.71 -1.1
3,205,296 + BWI76_RS15300 0.89 -0.0
3,205,560 - BWI76_RS15305 0.23 +0.9

Or see this region's nucleotide sequence