Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15040

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15030 and BWI76_RS15035 overlap by 16 nucleotidesBWI76_RS15035 and BWI76_RS15040 are separated by 31 nucleotidesBWI76_RS15040 and BWI76_RS15045 are separated by 92 nucleotidesBWI76_RS15045 and BWI76_RS15050 overlap by 18 nucleotides BWI76_RS15030: BWI76_RS15030 - ABC transporter, at 3,146,456 to 3,147,247 _RS15030 BWI76_RS15035: BWI76_RS15035 - metal ABC transporter permease, at 3,147,232 to 3,147,903 _RS15035 BWI76_RS15040: BWI76_RS15040 - putative 2-nitropropane dioxygenase, at 3,147,935 to 3,148,981 _RS15040 BWI76_RS15045: BWI76_RS15045 - LysR family transcriptional regulator, at 3,149,074 to 3,149,910 _RS15045 BWI76_RS15050: BWI76_RS15050 - sugar transporter, at 3,149,893 to 3,151,080 _RS15050 Position (kb) 3147 3148 3149Strain fitness (log2 ratio) -1 0 1 2 3 4 5at 3147.155 kb on - strand, within BWI76_RS15030at 3147.407 kb on - strand, within BWI76_RS15035at 3147.752 kb on + strand, within BWI76_RS15035at 3148.203 kb on + strand, within BWI76_RS15040at 3148.263 kb on + strand, within BWI76_RS15040at 3148.302 kb on - strand, within BWI76_RS15040at 3148.304 kb on + strand, within BWI76_RS15040at 3148.304 kb on + strand, within BWI76_RS15040at 3148.317 kb on + strand, within BWI76_RS15040at 3148.389 kb on - strand, within BWI76_RS15040at 3148.478 kb on - strand, within BWI76_RS15040at 3148.509 kb on - strand, within BWI76_RS15040at 3148.603 kb on + strand, within BWI76_RS15040at 3149.322 kb on - strand, within BWI76_RS15045at 3149.332 kb on - strand, within BWI76_RS15045at 3149.625 kb on + strand, within BWI76_RS15045at 3149.848 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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3,147,155 - BWI76_RS15030 0.88 -0.6
3,147,407 - BWI76_RS15035 0.26 +4.8
3,147,752 + BWI76_RS15035 0.77 -0.4
3,148,203 + BWI76_RS15040 0.26 -0.0
3,148,263 + BWI76_RS15040 0.31 -0.8
3,148,302 - BWI76_RS15040 0.35 +0.2
3,148,304 + BWI76_RS15040 0.35 -1.0
3,148,304 + BWI76_RS15040 0.35 +0.0
3,148,317 + BWI76_RS15040 0.36 -0.7
3,148,389 - BWI76_RS15040 0.43 -0.0
3,148,478 - BWI76_RS15040 0.52 -0.4
3,148,509 - BWI76_RS15040 0.55 +4.3
3,148,603 + BWI76_RS15040 0.64 +0.4
3,149,322 - BWI76_RS15045 0.30 -0.2
3,149,332 - BWI76_RS15045 0.31 +0.3
3,149,625 + BWI76_RS15045 0.66 +0.4
3,149,848 - -0.5

Or see this region's nucleotide sequence