Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12250 and BWI76_RS12255 are separated by 4 nucleotides BWI76_RS12255 and BWI76_RS12260 overlap by 17 nucleotides
BWI76_RS12250: BWI76_RS12250 - terminase, at 2,559,721 to 2,560,989
_RS12250
BWI76_RS12255: BWI76_RS12255 - hypothetical protein, at 2,560,994 to 2,562,418
_RS12255
BWI76_RS12260: BWI76_RS12260 - hypothetical protein, at 2,562,402 to 2,563,514
_RS12260
Position (kb)
2560
2561
2562
2563 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7
8 at 2560.011 kb on + strand, within BWI76_RS12250 at 2560.019 kb on - strand, within BWI76_RS12250 at 2560.054 kb on + strand, within BWI76_RS12250 at 2560.175 kb on + strand, within BWI76_RS12250 at 2560.206 kb on + strand, within BWI76_RS12250 at 2560.234 kb on + strand, within BWI76_RS12250 at 2560.249 kb on + strand, within BWI76_RS12250 at 2560.314 kb on + strand, within BWI76_RS12250 at 2560.340 kb on - strand, within BWI76_RS12250 at 2560.340 kb on - strand, within BWI76_RS12250 at 2560.340 kb on - strand, within BWI76_RS12250 at 2560.384 kb on - strand, within BWI76_RS12250 at 2560.441 kb on + strand, within BWI76_RS12250 at 2560.469 kb on - strand, within BWI76_RS12250 at 2560.502 kb on + strand, within BWI76_RS12250 at 2560.510 kb on - strand, within BWI76_RS12250 at 2560.533 kb on + strand, within BWI76_RS12250 at 2560.540 kb on - strand, within BWI76_RS12250 at 2560.730 kb on + strand, within BWI76_RS12250 at 2560.776 kb on + strand, within BWI76_RS12250 at 2560.840 kb on - strand, within BWI76_RS12250 at 2561.024 kb on - strand at 2561.149 kb on + strand, within BWI76_RS12255 at 2561.171 kb on + strand, within BWI76_RS12255 at 2561.186 kb on - strand, within BWI76_RS12255 at 2561.220 kb on - strand, within BWI76_RS12255 at 2561.232 kb on - strand, within BWI76_RS12255 at 2561.237 kb on + strand, within BWI76_RS12255 at 2561.237 kb on + strand, within BWI76_RS12255 at 2561.247 kb on + strand, within BWI76_RS12255 at 2561.446 kb on + strand, within BWI76_RS12255 at 2561.446 kb on + strand, within BWI76_RS12255 at 2561.454 kb on - strand, within BWI76_RS12255 at 2561.477 kb on - strand, within BWI76_RS12255 at 2561.650 kb on + strand, within BWI76_RS12255 at 2561.717 kb on + strand, within BWI76_RS12255 at 2561.783 kb on - strand, within BWI76_RS12255 at 2561.784 kb on + strand, within BWI76_RS12255 at 2561.794 kb on + strand, within BWI76_RS12255 at 2562.083 kb on + strand, within BWI76_RS12255 at 2562.083 kb on + strand, within BWI76_RS12255 at 2562.091 kb on - strand, within BWI76_RS12255 at 2562.382 kb on + strand at 2562.390 kb on - strand at 2562.390 kb on - strand at 2562.607 kb on - strand, within BWI76_RS12260 at 2562.931 kb on - strand, within BWI76_RS12260 at 2563.018 kb on - strand, within BWI76_RS12260 at 2563.113 kb on - strand, within BWI76_RS12260 at 2563.173 kb on - strand, within BWI76_RS12260
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 2,560,011 + BWI76_RS12250 0.23 +8.3 2,560,019 - BWI76_RS12250 0.23 -1.3 2,560,054 + BWI76_RS12250 0.26 -1.1 2,560,175 + BWI76_RS12250 0.36 -1.2 2,560,206 + BWI76_RS12250 0.38 -1.0 2,560,234 + BWI76_RS12250 0.40 -2.4 2,560,249 + BWI76_RS12250 0.42 -2.2 2,560,314 + BWI76_RS12250 0.47 -2.4 2,560,340 - BWI76_RS12250 0.49 -2.1 2,560,340 - BWI76_RS12250 0.49 -1.1 2,560,340 - BWI76_RS12250 0.49 -1.4 2,560,384 - BWI76_RS12250 0.52 -1.7 2,560,441 + BWI76_RS12250 0.57 -0.3 2,560,469 - BWI76_RS12250 0.59 -0.8 2,560,502 + BWI76_RS12250 0.62 -1.2 2,560,510 - BWI76_RS12250 0.62 -0.7 2,560,533 + BWI76_RS12250 0.64 +0.0 2,560,540 - BWI76_RS12250 0.65 -1.8 2,560,730 + BWI76_RS12250 0.80 -1.0 2,560,776 + BWI76_RS12250 0.83 -1.1 2,560,840 - BWI76_RS12250 0.88 -2.3 2,561,024 - -0.9 2,561,149 + BWI76_RS12255 0.11 -2.3 2,561,171 + BWI76_RS12255 0.12 -1.9 2,561,186 - BWI76_RS12255 0.13 -1.6 2,561,220 - BWI76_RS12255 0.16 -0.8 2,561,232 - BWI76_RS12255 0.17 -4.0 2,561,237 + BWI76_RS12255 0.17 -1.3 2,561,237 + BWI76_RS12255 0.17 -1.1 2,561,247 + BWI76_RS12255 0.18 -1.6 2,561,446 + BWI76_RS12255 0.32 -1.1 2,561,446 + BWI76_RS12255 0.32 -1.4 2,561,454 - BWI76_RS12255 0.32 -1.0 2,561,477 - BWI76_RS12255 0.34 -2.7 2,561,650 + BWI76_RS12255 0.46 -1.6 2,561,717 + BWI76_RS12255 0.51 -0.6 2,561,783 - BWI76_RS12255 0.55 -1.4 2,561,784 + BWI76_RS12255 0.55 -4.0 2,561,794 + BWI76_RS12255 0.56 -2.1 2,562,083 + BWI76_RS12255 0.76 -0.5 2,562,083 + BWI76_RS12255 0.76 -1.0 2,562,091 - BWI76_RS12255 0.77 +3.2 2,562,382 + -0.3 2,562,390 - -2.6 2,562,390 - -1.5 2,562,607 - BWI76_RS12260 0.18 -1.5 2,562,931 - BWI76_RS12260 0.48 -1.3 2,563,018 - BWI76_RS12260 0.55 +0.3 2,563,113 - BWI76_RS12260 0.64 -0.6 2,563,173 - BWI76_RS12260 0.69 -1.8
Or see this region's nucleotide sequence