Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS11045

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS11040 and BWI76_RS11045 are separated by 307 nucleotidesBWI76_RS11045 and BWI76_RS11050 are separated by 189 nucleotides BWI76_RS11040: BWI76_RS11040 - ribonuclease E, at 2,328,509 to 2,331,757 _RS11040 BWI76_RS11045: BWI76_RS11045 - hypothetical protein, at 2,332,065 to 2,332,223 _RS11045 BWI76_RS11050: BWI76_RS11050 - 23S rRNA pseudouridine(955/2504/2580) synthase, at 2,332,413 to 2,333,408 _RS11050 Position (kb) 2332 2333Strain fitness (log2 ratio) -2 -1 0 1at 2332.246 kb on - strandat 2332.334 kb on - strandat 2332.375 kb on + strandat 2332.420 kb on + strandat 2332.541 kb on + strand, within BWI76_RS11050at 2332.547 kb on - strand, within BWI76_RS11050at 2332.547 kb on - strand, within BWI76_RS11050at 2332.549 kb on - strand, within BWI76_RS11050at 2332.549 kb on - strand, within BWI76_RS11050at 2332.585 kb on + strand, within BWI76_RS11050at 2332.699 kb on + strand, within BWI76_RS11050at 2332.806 kb on + strand, within BWI76_RS11050at 2332.813 kb on + strand, within BWI76_RS11050at 2332.821 kb on - strand, within BWI76_RS11050at 2332.989 kb on - strand, within BWI76_RS11050at 2333.186 kb on - strand, within BWI76_RS11050at 2333.211 kb on - strand, within BWI76_RS11050

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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2,332,246 - +0.5
2,332,334 - +0.2
2,332,375 + +0.1
2,332,420 + -0.2
2,332,541 + BWI76_RS11050 0.13 +0.9
2,332,547 - BWI76_RS11050 0.13 -0.3
2,332,547 - BWI76_RS11050 0.13 -2.2
2,332,549 - BWI76_RS11050 0.14 -0.6
2,332,549 - BWI76_RS11050 0.14 +1.1
2,332,585 + BWI76_RS11050 0.17 -0.6
2,332,699 + BWI76_RS11050 0.29 -0.3
2,332,806 + BWI76_RS11050 0.39 -0.1
2,332,813 + BWI76_RS11050 0.40 -1.5
2,332,821 - BWI76_RS11050 0.41 -0.8
2,332,989 - BWI76_RS11050 0.58 +0.5
2,333,186 - BWI76_RS11050 0.78 +0.8
2,333,211 - BWI76_RS11050 0.80 +0.4

Or see this region's nucleotide sequence