Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10645

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10640 and BWI76_RS10645 are separated by 251 nucleotidesBWI76_RS10645 and BWI76_RS10650 are separated by 125 nucleotidesBWI76_RS10650 and BWI76_RS10655 are separated by 15 nucleotides BWI76_RS10640: BWI76_RS10640 - AraC family transcriptional regulator, at 2,246,887 to 2,247,720 _RS10640 BWI76_RS10645: BWI76_RS10645 - phytanoyl-CoA dioxygenase, at 2,247,972 to 2,248,739 _RS10645 BWI76_RS10650: BWI76_RS10650 - PTS fructose-like transporter subunit EIIB, at 2,248,865 to 2,249,206 _RS10650 BWI76_RS10655: BWI76_RS10655 - [formate-C-acetyltransferase]-activating enzyme, at 2,249,222 to 2,250,097 _RS10655 Position (kb) 2247 2248 2249Strain fitness (log2 ratio) -1 0 1 2at 2246.992 kb on + strand, within BWI76_RS10640at 2247.121 kb on + strand, within BWI76_RS10640at 2247.148 kb on - strand, within BWI76_RS10640at 2247.575 kb on - strand, within BWI76_RS10640at 2247.644 kb on + strandat 2247.729 kb on + strandat 2247.737 kb on - strandat 2247.922 kb on + strandat 2248.058 kb on + strand, within BWI76_RS10645at 2248.058 kb on + strand, within BWI76_RS10645at 2248.058 kb on + strand, within BWI76_RS10645at 2248.071 kb on - strand, within BWI76_RS10645at 2248.354 kb on + strand, within BWI76_RS10645at 2248.411 kb on + strand, within BWI76_RS10645at 2248.419 kb on - strand, within BWI76_RS10645at 2248.419 kb on - strand, within BWI76_RS10645at 2248.488 kb on + strand, within BWI76_RS10645at 2249.028 kb on + strand, within BWI76_RS10650at 2249.048 kb on - strand, within BWI76_RS10650at 2249.148 kb on + strand, within BWI76_RS10650at 2249.150 kb on + strand, within BWI76_RS10650at 2249.156 kb on - strand, within BWI76_RS10650at 2249.156 kb on - strand, within BWI76_RS10650at 2249.389 kb on + strand, within BWI76_RS10655at 2249.389 kb on + strand, within BWI76_RS10655at 2249.389 kb on + strand, within BWI76_RS10655at 2249.397 kb on - strand, within BWI76_RS10655at 2249.397 kb on - strand, within BWI76_RS10655

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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2,246,992 + BWI76_RS10640 0.13 -1.4
2,247,121 + BWI76_RS10640 0.28 -0.2
2,247,148 - BWI76_RS10640 0.31 +0.0
2,247,575 - BWI76_RS10640 0.82 +0.4
2,247,644 + -0.2
2,247,729 + -0.6
2,247,737 - +0.2
2,247,922 + -0.2
2,248,058 + BWI76_RS10645 0.11 +0.3
2,248,058 + BWI76_RS10645 0.11 +1.1
2,248,058 + BWI76_RS10645 0.11 -0.1
2,248,071 - BWI76_RS10645 0.13 -0.3
2,248,354 + BWI76_RS10645 0.50 +1.1
2,248,411 + BWI76_RS10645 0.57 +1.1
2,248,419 - BWI76_RS10645 0.58 +2.1
2,248,419 - BWI76_RS10645 0.58 +1.3
2,248,488 + BWI76_RS10645 0.67 +0.2
2,249,028 + BWI76_RS10650 0.48 +0.0
2,249,048 - BWI76_RS10650 0.54 +0.3
2,249,148 + BWI76_RS10650 0.83 -0.0
2,249,150 + BWI76_RS10650 0.83 +1.0
2,249,156 - BWI76_RS10650 0.85 -1.0
2,249,156 - BWI76_RS10650 0.85 -1.2
2,249,389 + BWI76_RS10655 0.19 -1.2
2,249,389 + BWI76_RS10655 0.19 -0.6
2,249,389 + BWI76_RS10655 0.19 -0.1
2,249,397 - BWI76_RS10655 0.20 +0.5
2,249,397 - BWI76_RS10655 0.20 -0.2

Or see this region's nucleotide sequence