Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05310 and BWI76_RS05315 are separated by 15 nucleotides BWI76_RS05315 and BWI76_RS05320 overlap by 14 nucleotides BWI76_RS05320 and BWI76_RS05325 are separated by 196 nucleotides BWI76_RS05325 and BWI76_RS05330 overlap by 4 nucleotides BWI76_RS05330 and BWI76_RS05335 are separated by 20 nucleotides
BWI76_RS05310: BWI76_RS05310 - tRNA(Ile)-lysidine synthetase, at 1,120,373 to 1,121,680
_RS05310
BWI76_RS05315: BWI76_RS05315 - Rho-binding antiterminator, at 1,121,696 to 1,121,956
_RS05315
BWI76_RS05320: BWI76_RS05320 - hypothetical protein, at 1,121,943 to 1,122,143
_RS05320
BWI76_RS05325: BWI76_RS05325 - hypothetical protein, at 1,122,340 to 1,122,885
_RS05325
BWI76_RS05330: BWI76_RS05330 - aminoacyl-tRNA hydrolase, at 1,122,882 to 1,123,295
_RS05330
BWI76_RS05335: BWI76_RS05335 - copper homeostasis/adhesion lipoprotein NlpE, at 1,123,316 to 1,124,035
_RS05335
Position (kb) 1122
1123 Strain fitness (log2 ratio) -1
0
1
2
3 at 1121.826 kb on - strand, within BWI76_RS05315 at 1121.876 kb on + strand, within BWI76_RS05315 at 1121.876 kb on + strand, within BWI76_RS05315 at 1122.193 kb on - strand at 1122.206 kb on - strand at 1122.219 kb on + strand at 1122.463 kb on - strand, within BWI76_RS05325 at 1122.595 kb on - strand, within BWI76_RS05325 at 1122.743 kb on - strand, within BWI76_RS05325 at 1122.754 kb on - strand, within BWI76_RS05325 at 1122.762 kb on + strand, within BWI76_RS05325 at 1122.813 kb on - strand, within BWI76_RS05325 at 1122.813 kb on - strand, within BWI76_RS05325 at 1123.014 kb on + strand, within BWI76_RS05330 at 1123.167 kb on - strand, within BWI76_RS05330 at 1123.214 kb on - strand, within BWI76_RS05330 at 1123.296 kb on + strand at 1123.569 kb on + strand, within BWI76_RS05335 at 1123.875 kb on - strand, within BWI76_RS05335
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 1,121,826 - BWI76_RS05315 0.50 +1.9 1,121,876 + BWI76_RS05315 0.69 -0.6 1,121,876 + BWI76_RS05315 0.69 -0.7 1,122,193 - +1.4 1,122,206 - -0.1 1,122,219 + -0.1 1,122,463 - BWI76_RS05325 0.23 +3.2 1,122,595 - BWI76_RS05325 0.47 +0.3 1,122,743 - BWI76_RS05325 0.74 +0.8 1,122,754 - BWI76_RS05325 0.76 +0.1 1,122,762 + BWI76_RS05325 0.77 +1.9 1,122,813 - BWI76_RS05325 0.87 +0.4 1,122,813 - BWI76_RS05325 0.87 -0.2 1,123,014 + BWI76_RS05330 0.32 -0.0 1,123,167 - BWI76_RS05330 0.69 -0.3 1,123,214 - BWI76_RS05330 0.80 -0.4 1,123,296 + +3.1 1,123,569 + BWI76_RS05335 0.35 -0.0 1,123,875 - BWI76_RS05335 0.78 -0.8
Or see this region's nucleotide sequence