Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02185

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02175 and BWI76_RS02180 are separated by 69 nucleotidesBWI76_RS02180 and BWI76_RS02185 are separated by 33 nucleotidesBWI76_RS02185 and BWI76_RS02190 are separated by 14 nucleotidesBWI76_RS02190 and BWI76_RS02195 overlap by 1 nucleotides BWI76_RS02175: BWI76_RS02175 - ribose 5-phosphate isomerase B, at 452,524 to 452,973 _RS02175 BWI76_RS02180: BWI76_RS02180 - hypothetical protein, at 453,043 to 453,375 _RS02180 BWI76_RS02185: BWI76_RS02185 - phosphonate metabolism protein PhnP, at 453,409 to 454,167 _RS02185 BWI76_RS02190: BWI76_RS02190 - ribose 1,5-bisphosphokinase, at 454,182 to 454,745 _RS02190 BWI76_RS02195: BWI76_RS02195 - alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase, at 454,745 to 455,881 _RS02195 Position (kb) 453 454 455Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6 7 8at 452.413 kb on + strandat 452.566 kb on - strandat 452.611 kb on - strandat 452.664 kb on - strand, within BWI76_RS02175at 452.786 kb on - strand, within BWI76_RS02175at 452.813 kb on - strand, within BWI76_RS02175at 452.841 kb on - strand, within BWI76_RS02175at 452.869 kb on - strand, within BWI76_RS02175at 453.005 kb on + strandat 453.018 kb on - strandat 453.023 kb on + strandat 453.051 kb on + strandat 453.224 kb on - strand, within BWI76_RS02180at 453.304 kb on + strand, within BWI76_RS02180at 453.504 kb on + strandat 453.537 kb on + strand, within BWI76_RS02185at 453.705 kb on - strand, within BWI76_RS02185at 453.803 kb on - strand, within BWI76_RS02185at 453.803 kb on - strand, within BWI76_RS02185at 453.830 kb on + strand, within BWI76_RS02185at 453.848 kb on - strand, within BWI76_RS02185at 453.943 kb on + strand, within BWI76_RS02185at 454.169 kb on - strandat 454.274 kb on + strand, within BWI76_RS02190at 454.343 kb on + strand, within BWI76_RS02190at 454.559 kb on + strand, within BWI76_RS02190at 454.695 kb on + strandat 454.695 kb on + strandat 454.695 kb on + strandat 454.695 kb on + strandat 454.763 kb on - strandat 454.797 kb on + strandat 454.920 kb on - strandat 454.931 kb on + strand, within BWI76_RS02195at 455.083 kb on - strand, within BWI76_RS02195at 455.091 kb on - strand, within BWI76_RS02195

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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452,413 + -0.2
452,566 - -1.1
452,611 - -0.2
452,664 - BWI76_RS02175 0.31 -0.6
452,786 - BWI76_RS02175 0.58 -0.4
452,813 - BWI76_RS02175 0.64 -0.8
452,841 - BWI76_RS02175 0.70 +5.2
452,869 - BWI76_RS02175 0.77 -0.2
453,005 + -0.1
453,018 - -0.7
453,023 + +0.5
453,051 + +0.0
453,224 - BWI76_RS02180 0.54 -0.4
453,304 + BWI76_RS02180 0.78 +0.2
453,504 + +3.0
453,537 + BWI76_RS02185 0.17 -0.5
453,705 - BWI76_RS02185 0.39 +0.3
453,803 - BWI76_RS02185 0.52 -0.1
453,803 - BWI76_RS02185 0.52 -0.9
453,830 + BWI76_RS02185 0.55 -0.0
453,848 - BWI76_RS02185 0.58 +7.9
453,943 + BWI76_RS02185 0.70 -0.6
454,169 - +0.2
454,274 + BWI76_RS02190 0.16 -0.2
454,343 + BWI76_RS02190 0.29 -0.0
454,559 + BWI76_RS02190 0.67 -0.4
454,695 + +0.2
454,695 + +0.7
454,695 + +0.1
454,695 + +0.3
454,763 - +0.2
454,797 + -0.2
454,920 - -1.2
454,931 + BWI76_RS02195 0.16 -0.2
455,083 - BWI76_RS02195 0.30 +0.7
455,091 - BWI76_RS02195 0.30 -0.8

Or see this region's nucleotide sequence