Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00455 and BWI76_RS00460 overlap by 4 nucleotides BWI76_RS00460 and BWI76_RS00465 are separated by 12 nucleotides BWI76_RS00465 and BWI76_RS00470 are separated by 48 nucleotides BWI76_RS00470 and BWI76_RS00475 are separated by 156 nucleotides
BWI76_RS00455: BWI76_RS00455 - formate dehydrogenase cytochrome b556 subunit, at 93,214 to 93,849
_RS00455
BWI76_RS00460: BWI76_RS00460 - formate dehydrogenase subunit beta, at 93,846 to 94,748
_RS00460
BWI76_RS00465: BWI76_RS00465 - formate dehydrogenase-N subunit alpha, at 94,761 to 97,175
_RS00465
BWI76_RS00470: BWI76_RS00470 - sulfate ABC transporter substrate-binding protein, at 97,224 to 97,811
_RS00470
BWI76_RS00475: BWI76_RS00475 - sulfurtransferase FdhD, at 97,968 to 98,804
_RS00475
Position (kb) 94
95
96
97
98 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 94.744 kb on + strand at 94.789 kb on + strand at 94.789 kb on + strand at 95.082 kb on - strand, within BWI76_RS00465 at 95.176 kb on - strand, within BWI76_RS00465 at 95.176 kb on - strand, within BWI76_RS00465 at 95.501 kb on + strand, within BWI76_RS00465 at 95.501 kb on + strand, within BWI76_RS00465 at 95.503 kb on - strand, within BWI76_RS00465 at 95.509 kb on - strand, within BWI76_RS00465 at 95.518 kb on + strand, within BWI76_RS00465 at 96.205 kb on + strand, within BWI76_RS00465 at 96.456 kb on - strand, within BWI76_RS00465 at 96.574 kb on + strand, within BWI76_RS00465 at 96.574 kb on + strand, within BWI76_RS00465 at 96.582 kb on - strand, within BWI76_RS00465 at 96.582 kb on - strand, within BWI76_RS00465 at 96.582 kb on - strand, within BWI76_RS00465 at 96.604 kb on + strand, within BWI76_RS00465 at 96.813 kb on - strand, within BWI76_RS00465 at 96.861 kb on + strand, within BWI76_RS00465 at 97.211 kb on + strand at 97.222 kb on + strand at 97.349 kb on + strand, within BWI76_RS00470 at 97.406 kb on + strand, within BWI76_RS00470 at 97.492 kb on - strand, within BWI76_RS00470 at 97.529 kb on + strand, within BWI76_RS00470 at 97.529 kb on + strand, within BWI76_RS00470 at 97.529 kb on + strand, within BWI76_RS00470 at 97.529 kb on + strand, within BWI76_RS00470 at 97.534 kb on + strand, within BWI76_RS00470 at 97.537 kb on - strand, within BWI76_RS00470 at 97.637 kb on + strand, within BWI76_RS00470 at 97.660 kb on + strand, within BWI76_RS00470 at 97.668 kb on - strand, within BWI76_RS00470 at 97.668 kb on - strand, within BWI76_RS00470 at 97.701 kb on - strand, within BWI76_RS00470 at 97.737 kb on + strand, within BWI76_RS00470 at 97.774 kb on + strand at 97.793 kb on + strand at 97.806 kb on - strand at 97.882 kb on - strand at 97.944 kb on - strand at 98.110 kb on - strand, within BWI76_RS00475
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 94,744 + +0.3 94,789 + +0.7 94,789 + +1.2 95,082 - BWI76_RS00465 0.13 -0.1 95,176 - BWI76_RS00465 0.17 -0.2 95,176 - BWI76_RS00465 0.17 +0.2 95,501 + BWI76_RS00465 0.31 -0.5 95,501 + BWI76_RS00465 0.31 -0.6 95,503 - BWI76_RS00465 0.31 -0.7 95,509 - BWI76_RS00465 0.31 -0.2 95,518 + BWI76_RS00465 0.31 -0.1 96,205 + BWI76_RS00465 0.60 -1.0 96,456 - BWI76_RS00465 0.70 +0.2 96,574 + BWI76_RS00465 0.75 -0.2 96,574 + BWI76_RS00465 0.75 +0.2 96,582 - BWI76_RS00465 0.75 +0.9 96,582 - BWI76_RS00465 0.75 +0.4 96,582 - BWI76_RS00465 0.75 +0.3 96,604 + BWI76_RS00465 0.76 -0.2 96,813 - BWI76_RS00465 0.85 +0.1 96,861 + BWI76_RS00465 0.87 -1.5 97,211 + -0.6 97,222 + +0.2 97,349 + BWI76_RS00470 0.21 -0.4 97,406 + BWI76_RS00470 0.31 -0.9 97,492 - BWI76_RS00470 0.46 -0.5 97,529 + BWI76_RS00470 0.52 +0.3 97,529 + BWI76_RS00470 0.52 -0.4 97,529 + BWI76_RS00470 0.52 +3.8 97,529 + BWI76_RS00470 0.52 -1.6 97,534 + BWI76_RS00470 0.53 +3.0 97,537 - BWI76_RS00470 0.53 -0.2 97,637 + BWI76_RS00470 0.70 +0.5 97,660 + BWI76_RS00470 0.74 -0.2 97,668 - BWI76_RS00470 0.76 +1.1 97,668 - BWI76_RS00470 0.76 +0.5 97,701 - BWI76_RS00470 0.81 +0.5 97,737 + BWI76_RS00470 0.87 -0.2 97,774 + +1.4 97,793 + -0.5 97,806 - +5.3 97,882 - -0.1 97,944 - +1.0 98,110 - BWI76_RS00475 0.17 +0.2
Or see this region's nucleotide sequence