Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00290 and BWI76_RS00295 are separated by 12 nucleotides BWI76_RS00295 and BWI76_RS00300 overlap by 4 nucleotides BWI76_RS00300 and BWI76_RS00305 are separated by 3 nucleotides
BWI76_RS00290: BWI76_RS00290 - Ribokinase (EC 2.7.1.15) <small>(from data)</small>, at 60,235 to 61,164
_RS00290
BWI76_RS00295: BWI76_RS00295 - transcriptional regulator RbsR, at 61,177 to 62,160
_RS00295
BWI76_RS00300: BWI76_RS00300 - MFS transporter, at 62,157 to 63,554
_RS00300
BWI76_RS00305: BWI76_RS00305 - GntR family transcriptional regulator, at 63,558 to 64,268
_RS00305
Position (kb) 62
63
64 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 61.165 kb on + strand at 61.173 kb on - strand at 61.431 kb on + strand, within BWI76_RS00295 at 61.433 kb on + strand, within BWI76_RS00295 at 61.439 kb on + strand, within BWI76_RS00295 at 61.441 kb on - strand, within BWI76_RS00295 at 61.441 kb on - strand, within BWI76_RS00295 at 61.447 kb on - strand, within BWI76_RS00295 at 61.457 kb on - strand, within BWI76_RS00295 at 61.580 kb on - strand, within BWI76_RS00295 at 61.960 kb on + strand, within BWI76_RS00295 at 61.960 kb on + strand, within BWI76_RS00295 at 61.968 kb on - strand, within BWI76_RS00295 at 62.007 kb on + strand, within BWI76_RS00295 at 62.102 kb on + strand at 62.115 kb on - strand at 62.137 kb on - strand at 62.143 kb on - strand at 62.163 kb on - strand at 62.169 kb on - strand at 62.273 kb on + strand at 62.429 kb on + strand, within BWI76_RS00300 at 62.470 kb on + strand, within BWI76_RS00300 at 62.584 kb on - strand, within BWI76_RS00300 at 62.599 kb on - strand, within BWI76_RS00300 at 62.633 kb on + strand, within BWI76_RS00300 at 62.673 kb on + strand, within BWI76_RS00300 at 62.730 kb on - strand, within BWI76_RS00300 at 62.814 kb on - strand, within BWI76_RS00300 at 63.029 kb on - strand, within BWI76_RS00300 at 63.075 kb on + strand, within BWI76_RS00300 at 63.088 kb on - strand, within BWI76_RS00300 at 63.098 kb on - strand, within BWI76_RS00300 at 63.110 kb on - strand, within BWI76_RS00300 at 63.115 kb on - strand, within BWI76_RS00300 at 63.129 kb on - strand, within BWI76_RS00300 at 63.129 kb on - strand, within BWI76_RS00300 at 63.130 kb on - strand, within BWI76_RS00300 at 63.404 kb on - strand, within BWI76_RS00300 at 63.429 kb on + strand at 63.442 kb on - strand at 63.460 kb on + strand at 63.519 kb on - strand at 63.526 kb on + strand at 63.636 kb on + strand, within BWI76_RS00305 at 63.668 kb on + strand, within BWI76_RS00305 at 63.690 kb on - strand, within BWI76_RS00305 at 63.706 kb on + strand, within BWI76_RS00305 at 63.722 kb on - strand, within BWI76_RS00305 at 63.811 kb on - strand, within BWI76_RS00305 at 63.862 kb on + strand, within BWI76_RS00305 at 63.862 kb on - strand, within BWI76_RS00305 at 63.870 kb on - strand, within BWI76_RS00305 at 63.923 kb on + strand, within BWI76_RS00305 at 63.928 kb on + strand, within BWI76_RS00305 at 63.931 kb on - strand, within BWI76_RS00305 at 63.941 kb on - strand, within BWI76_RS00305 at 63.962 kb on + strand, within BWI76_RS00305 at 63.962 kb on - strand, within BWI76_RS00305 at 63.980 kb on - strand, within BWI76_RS00305 at 64.063 kb on + strand, within BWI76_RS00305 at 64.076 kb on + strand, within BWI76_RS00305 at 64.117 kb on - strand, within BWI76_RS00305 at 64.150 kb on - strand, within BWI76_RS00305 at 64.212 kb on - strand at 64.233 kb on - strand at 64.251 kb on - strand at 64.274 kb on - strand at 64.293 kb on + strand at 64.433 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 61,165 + -0.1 61,173 - -0.1 61,431 + BWI76_RS00295 0.26 -0.3 61,433 + BWI76_RS00295 0.26 -1.0 61,439 + BWI76_RS00295 0.27 -0.5 61,441 - BWI76_RS00295 0.27 -2.6 61,441 - BWI76_RS00295 0.27 -1.5 61,447 - BWI76_RS00295 0.27 -1.2 61,457 - BWI76_RS00295 0.28 -1.3 61,580 - BWI76_RS00295 0.41 -2.1 61,960 + BWI76_RS00295 0.80 +3.5 61,960 + BWI76_RS00295 0.80 +0.5 61,968 - BWI76_RS00295 0.80 -0.4 62,007 + BWI76_RS00295 0.84 -0.0 62,102 + -1.1 62,115 - -1.4 62,137 - -1.1 62,143 - -1.4 62,163 - +0.1 62,169 - +0.2 62,273 + -1.1 62,429 + BWI76_RS00300 0.19 +5.9 62,470 + BWI76_RS00300 0.22 +0.3 62,584 - BWI76_RS00300 0.31 -0.8 62,599 - BWI76_RS00300 0.32 +0.4 62,633 + BWI76_RS00300 0.34 +0.0 62,673 + BWI76_RS00300 0.37 -1.7 62,730 - BWI76_RS00300 0.41 +2.0 62,814 - BWI76_RS00300 0.47 +0.5 63,029 - BWI76_RS00300 0.62 +1.1 63,075 + BWI76_RS00300 0.66 +0.3 63,088 - BWI76_RS00300 0.67 +0.4 63,098 - BWI76_RS00300 0.67 +0.1 63,110 - BWI76_RS00300 0.68 +0.8 63,115 - BWI76_RS00300 0.69 -0.3 63,129 - BWI76_RS00300 0.70 -0.1 63,129 - BWI76_RS00300 0.70 +0.8 63,130 - BWI76_RS00300 0.70 -1.9 63,404 - BWI76_RS00300 0.89 -0.2 63,429 + -0.9 63,442 - +0.1 63,460 + +0.9 63,519 - -0.6 63,526 + -1.8 63,636 + BWI76_RS00305 0.11 -0.7 63,668 + BWI76_RS00305 0.15 +0.5 63,690 - BWI76_RS00305 0.19 -0.1 63,706 + BWI76_RS00305 0.21 -0.7 63,722 - BWI76_RS00305 0.23 -0.3 63,811 - BWI76_RS00305 0.36 +0.6 63,862 + BWI76_RS00305 0.43 -0.5 63,862 - BWI76_RS00305 0.43 -0.3 63,870 - BWI76_RS00305 0.44 -1.0 63,923 + BWI76_RS00305 0.51 +0.0 63,928 + BWI76_RS00305 0.52 -1.4 63,931 - BWI76_RS00305 0.52 -2.0 63,941 - BWI76_RS00305 0.54 -0.4 63,962 + BWI76_RS00305 0.57 -0.2 63,962 - BWI76_RS00305 0.57 -0.7 63,980 - BWI76_RS00305 0.59 -0.5 64,063 + BWI76_RS00305 0.71 -0.7 64,076 + BWI76_RS00305 0.73 -0.2 64,117 - BWI76_RS00305 0.79 +0.7 64,150 - BWI76_RS00305 0.83 -0.2 64,212 - +0.1 64,233 - +2.7 64,251 - -0.3 64,274 - +3.3 64,293 + -0.6 64,433 - -0.2
Or see this region's nucleotide sequence