Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23025 and BWI76_RS23030 are separated by 1 nucleotides BWI76_RS23030 and BWI76_RS23035 overlap by 4 nucleotides BWI76_RS23035 and BWI76_RS23040 overlap by 4 nucleotides BWI76_RS23040 and BWI76_RS23045 are separated by 10 nucleotides
BWI76_RS23025: BWI76_RS23025 - cobalt ABC transporter substrate-binding protein CbiN, at 4,803,523 to 4,803,804
_RS23025
BWI76_RS23030: BWI76_RS23030 - cobalamin biosynthesis protein CbiM, at 4,803,806 to 4,804,543
_RS23030
BWI76_RS23035: BWI76_RS23035 - precorrin-2 C(20)-methyltransferase, at 4,804,540 to 4,805,253
_RS23035
BWI76_RS23040: BWI76_RS23040 - sirohydrochlorin cobaltochelatase, at 4,805,250 to 4,806,044
_RS23040
BWI76_RS23045: BWI76_RS23045 - cobalt-precorrin-6A/precorrin-6x reductase, at 4,806,055 to 4,806,834
_RS23045
Position (kb) 4804
4805
4806 Strain fitness (log2 ratio) -2
-1
0
1 at 4803.606 kb on - strand, within BWI76_RS23025 at 4803.768 kb on - strand, within BWI76_RS23025 at 4804.086 kb on - strand, within BWI76_RS23030 at 4804.305 kb on + strand, within BWI76_RS23030 at 4804.429 kb on + strand at 4804.681 kb on - strand, within BWI76_RS23035 at 4804.823 kb on + strand, within BWI76_RS23035 at 4804.904 kb on - strand, within BWI76_RS23035 at 4804.994 kb on + strand, within BWI76_RS23035 at 4805.015 kb on + strand, within BWI76_RS23035 at 4805.376 kb on - strand, within BWI76_RS23040 at 4805.517 kb on + strand, within BWI76_RS23040 at 4805.547 kb on + strand, within BWI76_RS23040 at 4805.728 kb on - strand, within BWI76_RS23040 at 4805.730 kb on + strand, within BWI76_RS23040 at 4805.756 kb on - strand, within BWI76_RS23040 at 4805.863 kb on + strand, within BWI76_RS23040 at 4805.888 kb on + strand, within BWI76_RS23040 at 4806.162 kb on + strand, within BWI76_RS23045
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 4,803,606 - BWI76_RS23025 0.29 +0.6 4,803,768 - BWI76_RS23025 0.87 -0.2 4,804,086 - BWI76_RS23030 0.38 -0.5 4,804,305 + BWI76_RS23030 0.68 +0.6 4,804,429 + +1.4 4,804,681 - BWI76_RS23035 0.20 -1.9 4,804,823 + BWI76_RS23035 0.40 +0.5 4,804,904 - BWI76_RS23035 0.51 -0.7 4,804,994 + BWI76_RS23035 0.64 -0.2 4,805,015 + BWI76_RS23035 0.67 -1.7 4,805,376 - BWI76_RS23040 0.16 -0.4 4,805,517 + BWI76_RS23040 0.34 -0.1 4,805,547 + BWI76_RS23040 0.37 -0.5 4,805,728 - BWI76_RS23040 0.60 -1.1 4,805,730 + BWI76_RS23040 0.60 -0.3 4,805,756 - BWI76_RS23040 0.64 -0.1 4,805,863 + BWI76_RS23040 0.77 -0.9 4,805,888 + BWI76_RS23040 0.80 -1.2 4,806,162 + BWI76_RS23045 0.14 -0.2
Or see this region's nucleotide sequence