Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01245 and BWI76_RS01250 are separated by 61 nucleotides BWI76_RS01250 and BWI76_RS01255 are separated by 246 nucleotides BWI76_RS01255 and BWI76_RS01260 are separated by 109 nucleotides
BWI76_RS01245: BWI76_RS01245 - DNA helicase RecQ, at 254,595 to 256,421
_RS01245
BWI76_RS01250: BWI76_RS01250 - threonine export protein RhtC, at 256,483 to 257,106
_RS01250
BWI76_RS01255: BWI76_RS01255 - homoserine/homoserine lactone efflux protein, at 257,353 to 257,973
_RS01255
BWI76_RS01260: BWI76_RS01260 - lysophospholipase L2, at 258,083 to 259,075
_RS01260
Position (kb) 257
258 Strain fitness (log2 ratio) -2
-1
0
1
2 at 256.357 kb on - strand at 256.376 kb on - strand at 256.408 kb on + strand at 256.535 kb on + strand at 256.543 kb on - strand at 256.633 kb on - strand, within BWI76_RS01250 at 256.643 kb on + strand, within BWI76_RS01250 at 256.643 kb on + strand, within BWI76_RS01250 at 256.651 kb on - strand, within BWI76_RS01250 at 256.656 kb on - strand, within BWI76_RS01250 at 256.718 kb on + strand, within BWI76_RS01250 at 256.807 kb on - strand, within BWI76_RS01250 at 256.875 kb on - strand, within BWI76_RS01250 at 257.129 kb on + strand at 257.137 kb on - strand at 257.179 kb on + strand at 257.379 kb on - strand at 257.379 kb on - strand at 257.464 kb on - strand, within BWI76_RS01255 at 257.533 kb on + strand, within BWI76_RS01255 at 257.543 kb on + strand, within BWI76_RS01255 at 257.551 kb on - strand, within BWI76_RS01255 at 257.687 kb on - strand, within BWI76_RS01255 at 257.689 kb on + strand, within BWI76_RS01255 at 257.696 kb on + strand, within BWI76_RS01255 at 257.761 kb on + strand, within BWI76_RS01255 at 257.769 kb on - strand, within BWI76_RS01255 at 257.811 kb on + strand, within BWI76_RS01255 at 257.853 kb on - strand at 258.014 kb on - strand at 258.080 kb on - strand at 258.332 kb on - strand, within BWI76_RS01260 at 258.485 kb on + strand, within BWI76_RS01260 at 258.620 kb on + strand, within BWI76_RS01260 at 258.620 kb on + strand, within BWI76_RS01260 at 258.628 kb on - strand, within BWI76_RS01260 at 258.689 kb on + strand, within BWI76_RS01260 at 258.907 kb on - strand, within BWI76_RS01260 at 258.955 kb on - strand, within BWI76_RS01260 at 258.963 kb on + strand, within BWI76_RS01260
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 256,357 - -0.6 256,376 - +0.2 256,408 + -0.2 256,535 + -0.4 256,543 - +0.9 256,633 - BWI76_RS01250 0.24 +1.0 256,643 + BWI76_RS01250 0.26 -0.1 256,643 + BWI76_RS01250 0.26 +0.0 256,651 - BWI76_RS01250 0.27 -1.5 256,656 - BWI76_RS01250 0.28 -1.2 256,718 + BWI76_RS01250 0.38 +0.7 256,807 - BWI76_RS01250 0.52 -0.8 256,875 - BWI76_RS01250 0.63 -1.0 257,129 + +0.8 257,137 - -1.5 257,179 + -0.0 257,379 - +2.1 257,379 - -0.2 257,464 - BWI76_RS01255 0.18 -0.2 257,533 + BWI76_RS01255 0.29 -0.2 257,543 + BWI76_RS01255 0.31 -0.1 257,551 - BWI76_RS01255 0.32 -0.2 257,687 - BWI76_RS01255 0.54 +0.3 257,689 + BWI76_RS01255 0.54 +0.6 257,696 + BWI76_RS01255 0.55 +0.1 257,761 + BWI76_RS01255 0.66 -0.5 257,769 - BWI76_RS01255 0.67 -0.6 257,811 + BWI76_RS01255 0.74 -0.1 257,853 - +0.2 258,014 - +0.7 258,080 - -2.5 258,332 - BWI76_RS01260 0.25 -0.7 258,485 + BWI76_RS01260 0.40 -0.4 258,620 + BWI76_RS01260 0.54 -0.0 258,620 + BWI76_RS01260 0.54 +0.3 258,628 - BWI76_RS01260 0.55 -1.3 258,689 + BWI76_RS01260 0.61 -0.1 258,907 - BWI76_RS01260 0.83 +0.5 258,955 - BWI76_RS01260 0.88 -0.8 258,963 + BWI76_RS01260 0.89 -1.2
Or see this region's nucleotide sequence