Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26915 and BWI76_RS26920 are separated by 159 nucleotides BWI76_RS26920 and BWI76_RS26925 overlap by 23 nucleotides BWI76_RS26925 and BWI76_RS26930 overlap by 38 nucleotides
BWI76_RS26915: BWI76_RS26915 - hypothetical protein, at 5,583,536 to 5,584,180
_RS26915
BWI76_RS26920: BWI76_RS26920 - DNA-3-methyladenine glycosylase I, at 5,584,340 to 5,584,921
_RS26920
BWI76_RS26925: BWI76_RS26925 - N-acetyltransferase, at 5,584,899 to 5,585,345
_RS26925
BWI76_RS26930: BWI76_RS26930 - biotin sulfoxide reductase, at 5,585,308 to 5,587,638
_RS26930
Position (kb) 5584
5585
5586 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 5584.018 kb on + strand, within BWI76_RS26915 at 5584.028 kb on + strand, within BWI76_RS26915 at 5584.031 kb on - strand, within BWI76_RS26915 at 5584.036 kb on - strand, within BWI76_RS26915 at 5584.038 kb on - strand, within BWI76_RS26915 at 5584.043 kb on - strand, within BWI76_RS26915 at 5584.050 kb on + strand, within BWI76_RS26915 at 5584.050 kb on + strand, within BWI76_RS26915 at 5584.082 kb on - strand, within BWI76_RS26915 at 5584.084 kb on + strand, within BWI76_RS26915 at 5584.116 kb on + strand at 5584.149 kb on + strand at 5584.208 kb on - strand at 5584.258 kb on + strand at 5584.329 kb on + strand at 5584.378 kb on - strand at 5584.378 kb on - strand at 5584.515 kb on + strand, within BWI76_RS26920 at 5584.533 kb on - strand, within BWI76_RS26920 at 5584.571 kb on + strand, within BWI76_RS26920 at 5584.830 kb on + strand, within BWI76_RS26920 at 5584.830 kb on + strand, within BWI76_RS26920 at 5584.840 kb on + strand, within BWI76_RS26920 at 5584.848 kb on - strand, within BWI76_RS26920 at 5584.852 kb on + strand, within BWI76_RS26920 at 5584.900 kb on - strand at 5585.003 kb on - strand, within BWI76_RS26925 at 5585.097 kb on - strand, within BWI76_RS26925 at 5585.165 kb on + strand, within BWI76_RS26925 at 5585.200 kb on + strand at 5585.200 kb on + strand, within BWI76_RS26925 at 5585.200 kb on + strand, within BWI76_RS26925 at 5585.200 kb on + strand, within BWI76_RS26925 at 5585.200 kb on + strand, within BWI76_RS26925 at 5585.200 kb on + strand, within BWI76_RS26925 at 5585.208 kb on - strand, within BWI76_RS26925 at 5585.208 kb on - strand, within BWI76_RS26925 at 5585.208 kb on - strand, within BWI76_RS26925 at 5585.208 kb on - strand, within BWI76_RS26925 at 5585.208 kb on - strand, within BWI76_RS26925 at 5585.208 kb on - strand, within BWI76_RS26925 at 5585.208 kb on - strand, within BWI76_RS26925 at 5585.244 kb on - strand, within BWI76_RS26925 at 5585.255 kb on - strand, within BWI76_RS26925 at 5585.312 kb on + strand at 5585.475 kb on + strand at 5585.625 kb on + strand, within BWI76_RS26930 at 5585.633 kb on - strand, within BWI76_RS26930 at 5585.726 kb on + strand, within BWI76_RS26930 at 5585.747 kb on - strand, within BWI76_RS26930
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 5,584,018 + BWI76_RS26915 0.75 +0.6 5,584,028 + BWI76_RS26915 0.76 -0.3 5,584,031 - BWI76_RS26915 0.77 +0.5 5,584,036 - BWI76_RS26915 0.78 -0.2 5,584,038 - BWI76_RS26915 0.78 +0.0 5,584,043 - BWI76_RS26915 0.79 +0.8 5,584,050 + BWI76_RS26915 0.80 -2.9 5,584,050 + BWI76_RS26915 0.80 -0.1 5,584,082 - BWI76_RS26915 0.85 +0.4 5,584,084 + BWI76_RS26915 0.85 +0.6 5,584,116 + -0.8 5,584,149 + -0.7 5,584,208 - +3.8 5,584,258 + -0.9 5,584,329 + -0.1 5,584,378 - -0.5 5,584,378 - -1.7 5,584,515 + BWI76_RS26920 0.30 -0.3 5,584,533 - BWI76_RS26920 0.33 -1.5 5,584,571 + BWI76_RS26920 0.40 +0.4 5,584,830 + BWI76_RS26920 0.84 -0.1 5,584,830 + BWI76_RS26920 0.84 -1.7 5,584,840 + BWI76_RS26920 0.86 -2.5 5,584,848 - BWI76_RS26920 0.87 -0.4 5,584,852 + BWI76_RS26920 0.88 -0.5 5,584,900 - -0.6 5,585,003 - BWI76_RS26925 0.23 +0.4 5,585,097 - BWI76_RS26925 0.44 -0.3 5,585,165 + BWI76_RS26925 0.60 -0.1 5,585,200 + +0.4 5,585,200 + BWI76_RS26925 0.67 +0.2 5,585,200 + BWI76_RS26925 0.67 -3.0 5,585,200 + BWI76_RS26925 0.67 +3.9 5,585,200 + BWI76_RS26925 0.67 -2.3 5,585,200 + BWI76_RS26925 0.67 +1.3 5,585,208 - BWI76_RS26925 0.69 +0.8 5,585,208 - BWI76_RS26925 0.69 -0.1 5,585,208 - BWI76_RS26925 0.69 -1.9 5,585,208 - BWI76_RS26925 0.69 -0.4 5,585,208 - BWI76_RS26925 0.69 +1.5 5,585,208 - BWI76_RS26925 0.69 -1.0 5,585,208 - BWI76_RS26925 0.69 +2.4 5,585,244 - BWI76_RS26925 0.77 -1.6 5,585,255 - BWI76_RS26925 0.80 -0.7 5,585,312 + -0.8 5,585,475 + -0.3 5,585,625 + BWI76_RS26930 0.14 +0.6 5,585,633 - BWI76_RS26930 0.14 -0.7 5,585,726 + BWI76_RS26930 0.18 +0.3 5,585,747 - BWI76_RS26930 0.19 -1.8
Or see this region's nucleotide sequence