Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21700 and BWI76_RS21705 are separated by 87 nucleotides BWI76_RS21705 and BWI76_RS21710 are separated by 171 nucleotides BWI76_RS21710 and BWI76_RS21715 overlap by 10 nucleotides BWI76_RS21715 and BWI76_RS21720 are separated by 1 nucleotides BWI76_RS21720 and BWI76_RS21725 overlap by 52 nucleotides
BWI76_RS21700: BWI76_RS21700 - AraC family transcriptional regulator, at 4,538,613 to 4,539,407
_RS21700
BWI76_RS21705: BWI76_RS21705 - MFS transporter, at 4,539,495 to 4,540,685
_RS21705
BWI76_RS21710: BWI76_RS21710 - nitroreductase, at 4,540,857 to 4,541,231
_RS21710
BWI76_RS21715: BWI76_RS21715 - transcriptional regulator, at 4,541,222 to 4,541,401
_RS21715
BWI76_RS21720: BWI76_RS21720 - hypothetical protein, at 4,541,403 to 4,541,645
_RS21720
BWI76_RS21725: BWI76_RS21725 - flavin reductase, at 4,541,594 to 4,542,166
_RS21725
Position (kb)
4539
4540
4541 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4538.611 kb on + strand at 4538.652 kb on - strand at 4538.662 kb on - strand at 4538.667 kb on - strand at 4538.703 kb on + strand, within BWI76_RS21700 at 4538.710 kb on + strand, within BWI76_RS21700 at 4538.715 kb on + strand, within BWI76_RS21700 at 4538.718 kb on + strand, within BWI76_RS21700 at 4538.723 kb on - strand, within BWI76_RS21700 at 4538.820 kb on + strand, within BWI76_RS21700 at 4538.849 kb on - strand, within BWI76_RS21700 at 4538.857 kb on - strand, within BWI76_RS21700 at 4538.895 kb on - strand, within BWI76_RS21700 at 4539.045 kb on + strand, within BWI76_RS21700 at 4539.168 kb on + strand, within BWI76_RS21700 at 4539.250 kb on - strand, within BWI76_RS21700 at 4539.369 kb on - strand at 4539.369 kb on - strand at 4539.374 kb on + strand at 4539.510 kb on + strand at 4539.626 kb on + strand, within BWI76_RS21705 at 4539.636 kb on - strand, within BWI76_RS21705 at 4539.682 kb on + strand, within BWI76_RS21705 at 4539.725 kb on - strand, within BWI76_RS21705 at 4539.725 kb on - strand, within BWI76_RS21705 at 4539.775 kb on + strand, within BWI76_RS21705 at 4539.788 kb on + strand, within BWI76_RS21705 at 4539.834 kb on + strand, within BWI76_RS21705 at 4539.842 kb on - strand, within BWI76_RS21705 at 4539.853 kb on + strand, within BWI76_RS21705 at 4539.943 kb on + strand, within BWI76_RS21705 at 4539.979 kb on - strand, within BWI76_RS21705 at 4540.116 kb on + strand, within BWI76_RS21705 at 4540.139 kb on + strand, within BWI76_RS21705 at 4540.212 kb on - strand, within BWI76_RS21705 at 4540.239 kb on - strand, within BWI76_RS21705 at 4540.404 kb on - strand, within BWI76_RS21705 at 4540.503 kb on + strand, within BWI76_RS21705 at 4540.689 kb on + strand at 4540.697 kb on - strand at 4540.697 kb on - strand at 4540.707 kb on - strand at 4540.724 kb on - strand at 4540.751 kb on - strand at 4540.772 kb on + strand at 4540.772 kb on + strand at 4540.772 kb on + strand at 4540.777 kb on + strand at 4540.782 kb on + strand at 4540.815 kb on + strand at 4540.855 kb on + strand at 4540.863 kb on - strand at 4540.863 kb on - strand at 4540.863 kb on - strand at 4540.868 kb on - strand at 4540.904 kb on - strand, within BWI76_RS21710 at 4540.925 kb on + strand, within BWI76_RS21710 at 4540.926 kb on - strand, within BWI76_RS21710 at 4541.051 kb on + strand, within BWI76_RS21710 at 4541.062 kb on + strand, within BWI76_RS21710 at 4541.083 kb on + strand, within BWI76_RS21710 at 4541.159 kb on + strand, within BWI76_RS21710 at 4541.249 kb on - strand, within BWI76_RS21715 at 4541.658 kb on + strand, within BWI76_RS21725 at 4541.673 kb on - strand, within BWI76_RS21725
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 4,538,611 + +1.3 4,538,652 - +2.1 4,538,662 - -1.9 4,538,667 - -1.2 4,538,703 + BWI76_RS21700 0.11 -0.5 4,538,710 + BWI76_RS21700 0.12 -0.1 4,538,715 + BWI76_RS21700 0.13 -0.6 4,538,718 + BWI76_RS21700 0.13 +0.8 4,538,723 - BWI76_RS21700 0.14 +0.9 4,538,820 + BWI76_RS21700 0.26 -0.9 4,538,849 - BWI76_RS21700 0.30 +0.5 4,538,857 - BWI76_RS21700 0.31 +0.8 4,538,895 - BWI76_RS21700 0.35 +0.6 4,539,045 + BWI76_RS21700 0.54 -1.1 4,539,168 + BWI76_RS21700 0.70 -0.5 4,539,250 - BWI76_RS21700 0.80 -2.1 4,539,369 - +0.4 4,539,369 - -0.2 4,539,374 + -2.5 4,539,510 + +0.7 4,539,626 + BWI76_RS21705 0.11 -2.1 4,539,636 - BWI76_RS21705 0.12 +1.7 4,539,682 + BWI76_RS21705 0.16 -3.3 4,539,725 - BWI76_RS21705 0.19 +0.9 4,539,725 - BWI76_RS21705 0.19 +1.8 4,539,775 + BWI76_RS21705 0.24 -0.3 4,539,788 + BWI76_RS21705 0.25 -2.0 4,539,834 + BWI76_RS21705 0.28 +2.3 4,539,842 - BWI76_RS21705 0.29 +1.2 4,539,853 + BWI76_RS21705 0.30 +0.0 4,539,943 + BWI76_RS21705 0.38 -0.6 4,539,979 - BWI76_RS21705 0.41 -2.0 4,540,116 + BWI76_RS21705 0.52 +0.6 4,540,139 + BWI76_RS21705 0.54 +2.8 4,540,212 - BWI76_RS21705 0.60 -1.0 4,540,239 - BWI76_RS21705 0.62 +1.8 4,540,404 - BWI76_RS21705 0.76 +0.1 4,540,503 + BWI76_RS21705 0.85 +0.5 4,540,689 + +1.2 4,540,697 - +0.2 4,540,697 - -2.1 4,540,707 - -0.6 4,540,724 - +0.7 4,540,751 - -0.7 4,540,772 + +0.3 4,540,772 + -1.9 4,540,772 + +0.5 4,540,777 + -0.2 4,540,782 + +0.4 4,540,815 + -0.4 4,540,855 + -3.1 4,540,863 - -1.0 4,540,863 - -0.3 4,540,863 - +2.6 4,540,868 - -0.5 4,540,904 - BWI76_RS21710 0.13 +1.2 4,540,925 + BWI76_RS21710 0.18 +0.7 4,540,926 - BWI76_RS21710 0.18 -2.2 4,541,051 + BWI76_RS21710 0.52 -1.6 4,541,062 + BWI76_RS21710 0.55 -2.0 4,541,083 + BWI76_RS21710 0.60 +0.9 4,541,159 + BWI76_RS21710 0.81 -0.1 4,541,249 - BWI76_RS21715 0.15 -0.8 4,541,658 + BWI76_RS21725 0.11 +0.7 4,541,673 - BWI76_RS21725 0.14 +4.6
Or see this region's nucleotide sequence