Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19210 and BWI76_RS19215 are separated by 84 nucleotides BWI76_RS19215 and BWI76_RS19220 overlap by 29 nucleotides BWI76_RS19220 and BWI76_RS19225 are separated by 14 nucleotides BWI76_RS19225 and BWI76_RS19230 are separated by 0 nucleotides
BWI76_RS19210: BWI76_RS19210 - LuxR family transcriptional regulator, at 3,998,342 to 3,999,094
_RS19210
BWI76_RS19215: BWI76_RS19215 - proline-specific peptidase, at 3,999,179 to 4,000,111
_RS19215
BWI76_RS19220: BWI76_RS19220 - hypothetical protein, at 4,000,083 to 4,001,201
_RS19220
BWI76_RS19225: BWI76_RS19225 - AsnC family transcriptional regulator, at 4,001,216 to 4,001,671
_RS19225
BWI76_RS19230: BWI76_RS19230 - polar amino acid ABC transporter inner membrane subunit, at 4,001,672 to 4,002,397
_RS19230
Position (kb) 4000
4001
4002 Strain fitness (log2 ratio) -1
0
1
2 at 3999.134 kb on + strand at 3999.570 kb on - strand, within BWI76_RS19215 at 4000.261 kb on + strand, within BWI76_RS19220 at 4000.475 kb on + strand, within BWI76_RS19220 at 4000.475 kb on + strand, within BWI76_RS19220 at 4000.760 kb on - strand, within BWI76_RS19220 at 4000.969 kb on + strand, within BWI76_RS19220 at 4001.044 kb on + strand, within BWI76_RS19220 at 4001.319 kb on - strand, within BWI76_RS19225 at 4001.345 kb on - strand, within BWI76_RS19225 at 4001.401 kb on + strand, within BWI76_RS19225 at 4001.570 kb on + strand, within BWI76_RS19225 at 4001.596 kb on - strand, within BWI76_RS19225 at 4001.845 kb on - strand at 4001.930 kb on - strand, within BWI76_RS19230
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 3,999,134 + +1.1 3,999,570 - BWI76_RS19215 0.42 +1.8 4,000,261 + BWI76_RS19220 0.16 -1.0 4,000,475 + BWI76_RS19220 0.35 -1.4 4,000,475 + BWI76_RS19220 0.35 -1.1 4,000,760 - BWI76_RS19220 0.61 -1.4 4,000,969 + BWI76_RS19220 0.79 -0.2 4,001,044 + BWI76_RS19220 0.86 +0.2 4,001,319 - BWI76_RS19225 0.23 +0.4 4,001,345 - BWI76_RS19225 0.28 -0.5 4,001,401 + BWI76_RS19225 0.41 -1.6 4,001,570 + BWI76_RS19225 0.78 -0.8 4,001,596 - BWI76_RS19225 0.83 +0.3 4,001,845 - -0.1 4,001,930 - BWI76_RS19230 0.36 +0.1
Or see this region's nucleotide sequence