Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18020 and BWI76_RS18025 are separated by 832 nucleotides BWI76_RS18025 and BWI76_RS18030 are separated by 22 nucleotides BWI76_RS18030 and BWI76_RS18035 are separated by 18 nucleotides
BWI76_RS18020: BWI76_RS18020 - virulence factor VirK, at 3,744,377 to 3,745,321
_RS18020
BWI76_RS18025: BWI76_RS18025 - spore coat U domain-containing protein, at 3,746,154 to 3,746,705
_RS18025
BWI76_RS18030: BWI76_RS18030 - spore coat protein U, at 3,746,728 to 3,747,246
_RS18030
BWI76_RS18035: BWI76_RS18035 - fimbrial chaperone, at 3,747,265 to 3,748,029
_RS18035
Position (kb)
3746
3747 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3745.277 kb on - strand at 3745.295 kb on + strand at 3745.324 kb on - strand at 3745.367 kb on - strand at 3745.369 kb on - strand at 3745.487 kb on + strand at 3745.523 kb on + strand at 3745.549 kb on - strand at 3745.554 kb on - strand at 3745.634 kb on + strand at 3745.642 kb on - strand at 3745.711 kb on + strand at 3745.722 kb on + strand at 3745.727 kb on + strand at 3745.727 kb on + strand at 3745.727 kb on + strand at 3745.727 kb on + strand at 3745.735 kb on - strand at 3745.735 kb on - strand at 3745.735 kb on - strand at 3745.735 kb on - strand at 3745.735 kb on - strand at 3745.738 kb on + strand at 3745.741 kb on - strand at 3745.746 kb on + strand at 3745.746 kb on + strand at 3745.746 kb on + strand at 3745.746 kb on + strand at 3745.746 kb on + strand at 3745.746 kb on - strand at 3745.754 kb on - strand at 3745.754 kb on - strand at 3745.767 kb on - strand at 3745.767 kb on - strand at 3745.777 kb on - strand at 3745.786 kb on - strand at 3745.791 kb on - strand at 3745.791 kb on - strand at 3745.791 kb on - strand at 3745.793 kb on + strand at 3745.801 kb on - strand at 3745.811 kb on - strand at 3745.811 kb on - strand at 3745.815 kb on + strand at 3745.815 kb on + strand at 3745.815 kb on + strand at 3745.815 kb on + strand at 3745.823 kb on - strand at 3745.823 kb on - strand at 3745.823 kb on - strand at 3745.823 kb on - strand at 3745.903 kb on + strand at 3745.984 kb on - strand at 3745.989 kb on - strand at 3746.062 kb on - strand at 3746.062 kb on - strand at 3746.093 kb on + strand at 3746.101 kb on - strand at 3746.147 kb on - strand at 3746.159 kb on + strand at 3746.159 kb on + strand at 3746.159 kb on + strand at 3746.179 kb on - strand at 3746.179 kb on - strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.181 kb on + strand at 3746.189 kb on - strand at 3746.189 kb on - strand at 3746.189 kb on - strand at 3746.189 kb on - strand at 3746.189 kb on - strand at 3746.189 kb on - strand at 3746.189 kb on - strand at 3746.240 kb on - strand, within BWI76_RS18025 at 3746.247 kb on - strand, within BWI76_RS18025 at 3746.268 kb on + strand, within BWI76_RS18025 at 3746.268 kb on + strand, within BWI76_RS18025 at 3746.356 kb on - strand, within BWI76_RS18025 at 3746.404 kb on + strand, within BWI76_RS18025 at 3746.475 kb on + strand, within BWI76_RS18025 at 3746.598 kb on + strand, within BWI76_RS18025 at 3746.691 kb on + strand at 3746.916 kb on + strand, within BWI76_RS18030 at 3746.928 kb on + strand, within BWI76_RS18030 at 3746.938 kb on + strand, within BWI76_RS18030 at 3746.994 kb on + strand, within BWI76_RS18030 at 3747.017 kb on + strand, within BWI76_RS18030 at 3747.048 kb on + strand, within BWI76_RS18030 at 3747.161 kb on - strand, within BWI76_RS18030 at 3747.163 kb on - strand, within BWI76_RS18030 at 3747.240 kb on - strand at 3747.383 kb on + strand, within BWI76_RS18035 at 3747.643 kb on + strand, within BWI76_RS18035 at 3747.677 kb on + strand, within BWI76_RS18035
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 3,745,277 - +0.0 3,745,295 + -0.2 3,745,324 - -0.5 3,745,367 - +1.2 3,745,369 - -1.6 3,745,487 + -1.9 3,745,523 + -2.1 3,745,549 - -2.2 3,745,554 - +0.0 3,745,634 + -0.4 3,745,642 - +1.0 3,745,711 + -3.5 3,745,722 + +0.8 3,745,727 + +0.3 3,745,727 + -0.6 3,745,727 + +0.4 3,745,727 + +1.1 3,745,735 - +0.6 3,745,735 - -1.2 3,745,735 - -0.4 3,745,735 - -1.7 3,745,735 - -0.4 3,745,738 + +0.1 3,745,741 - -0.1 3,745,746 + +0.1 3,745,746 + +0.7 3,745,746 + -0.2 3,745,746 + +0.2 3,745,746 + -0.6 3,745,746 - +1.1 3,745,754 - -1.1 3,745,754 - -0.5 3,745,767 - +0.2 3,745,767 - +0.6 3,745,777 - -1.8 3,745,786 - -1.6 3,745,791 - -0.5 3,745,791 - -0.5 3,745,791 - -2.6 3,745,793 + +0.2 3,745,801 - +0.1 3,745,811 - +0.5 3,745,811 - -0.7 3,745,815 + +0.7 3,745,815 + -3.6 3,745,815 + -0.2 3,745,815 + +0.1 3,745,823 - +0.1 3,745,823 - +0.4 3,745,823 - -0.9 3,745,823 - +0.6 3,745,903 + -0.0 3,745,984 - +0.6 3,745,989 - +0.4 3,746,062 - -0.6 3,746,062 - +0.9 3,746,093 + +0.0 3,746,101 - -1.1 3,746,147 - +0.5 3,746,159 + -0.5 3,746,159 + -0.6 3,746,159 + -0.5 3,746,179 - +0.0 3,746,179 - -0.8 3,746,181 + +0.2 3,746,181 + -0.5 3,746,181 + +0.9 3,746,181 + +1.1 3,746,181 + -3.5 3,746,181 + +0.2 3,746,181 + -0.3 3,746,181 + +0.1 3,746,181 + -1.6 3,746,181 + +0.1 3,746,181 + -0.5 3,746,181 + +1.2 3,746,189 - -0.2 3,746,189 - -0.5 3,746,189 - -1.4 3,746,189 - -0.2 3,746,189 - -0.6 3,746,189 - +0.3 3,746,189 - +1.4 3,746,240 - BWI76_RS18025 0.16 +1.3 3,746,247 - BWI76_RS18025 0.17 -1.2 3,746,268 + BWI76_RS18025 0.21 -0.5 3,746,268 + BWI76_RS18025 0.21 -0.3 3,746,356 - BWI76_RS18025 0.37 +2.8 3,746,404 + BWI76_RS18025 0.45 -1.6 3,746,475 + BWI76_RS18025 0.58 +0.1 3,746,598 + BWI76_RS18025 0.80 +0.6 3,746,691 + -0.6 3,746,916 + BWI76_RS18030 0.36 -0.5 3,746,928 + BWI76_RS18030 0.39 -0.0 3,746,938 + BWI76_RS18030 0.40 +1.2 3,746,994 + BWI76_RS18030 0.51 -0.8 3,747,017 + BWI76_RS18030 0.56 +2.6 3,747,048 + BWI76_RS18030 0.62 +0.7 3,747,161 - BWI76_RS18030 0.83 +0.4 3,747,163 - BWI76_RS18030 0.84 -0.1 3,747,240 - +0.1 3,747,383 + BWI76_RS18035 0.15 -0.8 3,747,643 + BWI76_RS18035 0.49 -0.4 3,747,677 + BWI76_RS18035 0.54 +0.7
Or see this region's nucleotide sequence