Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16740

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16735 and BWI76_RS16740 are separated by 48 nucleotidesBWI76_RS16740 and BWI76_RS16745 are separated by 106 nucleotidesBWI76_RS16745 and BWI76_RS16750 are separated by 10 nucleotides BWI76_RS16735: BWI76_RS16735 - cold-shock protein, at 3,467,232 to 3,467,444 _RS16735 BWI76_RS16740: BWI76_RS16740 - transcriptional regulator, at 3,467,493 to 3,468,365 _RS16740 BWI76_RS16745: BWI76_RS16745 - alpha-acetolactate decarboxylase, at 3,468,472 to 3,469,251 _RS16745 BWI76_RS16750: BWI76_RS16750 - acetolactate synthase, at 3,469,262 to 3,470,941 _RS16750 Position (kb) 3467 3468 3469Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3466.498 kb on - strandat 3466.569 kb on - strandat 3466.757 kb on + strandat 3466.763 kb on + strandat 3466.776 kb on - strandat 3466.791 kb on + strandat 3466.799 kb on - strandat 3466.819 kb on + strandat 3466.837 kb on - strandat 3466.853 kb on + strandat 3466.861 kb on + strandat 3466.861 kb on + strandat 3466.869 kb on + strandat 3466.945 kb on + strandat 3466.947 kb on + strandat 3466.963 kb on - strandat 3466.963 kb on - strandat 3466.963 kb on - strandat 3466.964 kb on + strandat 3466.964 kb on + strandat 3466.965 kb on - strandat 3466.969 kb on + strandat 3466.969 kb on + strandat 3466.969 kb on + strandat 3466.972 kb on - strandat 3466.977 kb on - strandat 3467.021 kb on + strandat 3467.029 kb on - strandat 3467.031 kb on - strandat 3467.073 kb on + strandat 3467.080 kb on - strandat 3467.184 kb on - strandat 3467.191 kb on + strandat 3467.199 kb on - strandat 3467.199 kb on - strandat 3467.199 kb on - strandat 3467.201 kb on - strandat 3467.201 kb on - strandat 3467.286 kb on + strand, within BWI76_RS16735at 3467.366 kb on - strand, within BWI76_RS16735at 3467.368 kb on + strand, within BWI76_RS16735at 3467.404 kb on - strand, within BWI76_RS16735at 3467.406 kb on + strand, within BWI76_RS16735at 3467.542 kb on - strandat 3467.583 kb on - strand, within BWI76_RS16740at 3467.764 kb on - strand, within BWI76_RS16740at 3468.038 kb on + strand, within BWI76_RS16740at 3468.038 kb on + strand, within BWI76_RS16740at 3468.205 kb on - strandat 3468.518 kb on - strandat 3468.524 kb on - strandat 3468.549 kb on - strandat 3468.559 kb on + strand, within BWI76_RS16745at 3468.576 kb on - strand, within BWI76_RS16745at 3468.576 kb on - strand, within BWI76_RS16745at 3468.585 kb on + strand, within BWI76_RS16745at 3468.585 kb on + strand, within BWI76_RS16745at 3468.800 kb on + strand, within BWI76_RS16745at 3469.030 kb on - strand, within BWI76_RS16745at 3469.079 kb on + strand, within BWI76_RS16745at 3469.307 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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3,466,498 - -0.2
3,466,569 - +1.3
3,466,757 + -0.7
3,466,763 + +1.2
3,466,776 - -0.1
3,466,791 + +0.1
3,466,799 - +1.4
3,466,819 + -1.7
3,466,837 - +1.3
3,466,853 + -3.4
3,466,861 + +0.6
3,466,861 + +0.9
3,466,869 + -0.2
3,466,945 + -1.1
3,466,947 + -2.9
3,466,963 - +0.9
3,466,963 - -0.6
3,466,963 - -0.1
3,466,964 + +0.5
3,466,964 + -1.2
3,466,965 - +2.4
3,466,969 + -3.9
3,466,969 + -0.9
3,466,969 + +0.6
3,466,972 - -0.3
3,466,977 - -0.5
3,467,021 + -0.2
3,467,029 - +0.2
3,467,031 - +0.6
3,467,073 + -1.2
3,467,080 - -0.7
3,467,184 - -1.9
3,467,191 + -3.3
3,467,199 - -1.0
3,467,199 - +0.4
3,467,199 - -1.0
3,467,201 - +0.5
3,467,201 - +0.2
3,467,286 + BWI76_RS16735 0.25 -0.3
3,467,366 - BWI76_RS16735 0.63 -1.5
3,467,368 + BWI76_RS16735 0.64 +1.4
3,467,404 - BWI76_RS16735 0.81 +0.2
3,467,406 + BWI76_RS16735 0.82 -1.2
3,467,542 - +0.1
3,467,583 - BWI76_RS16740 0.10 -1.2
3,467,764 - BWI76_RS16740 0.31 -0.7
3,468,038 + BWI76_RS16740 0.62 -0.2
3,468,038 + BWI76_RS16740 0.62 -0.9
3,468,205 - +0.1
3,468,518 - -0.8
3,468,524 - -0.8
3,468,549 - +0.9
3,468,559 + BWI76_RS16745 0.11 -2.6
3,468,576 - BWI76_RS16745 0.13 -1.6
3,468,576 - BWI76_RS16745 0.13 +0.0
3,468,585 + BWI76_RS16745 0.14 -1.3
3,468,585 + BWI76_RS16745 0.14 +0.1
3,468,800 + BWI76_RS16745 0.42 -1.0
3,469,030 - BWI76_RS16745 0.72 -2.7
3,469,079 + BWI76_RS16745 0.78 -0.3
3,469,307 - -0.1

Or see this region's nucleotide sequence