Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02205 and BWI76_RS02210 overlap by 4 nucleotides BWI76_RS02210 and BWI76_RS02215 overlap by 8 nucleotides BWI76_RS02215 and BWI76_RS02220 overlap by 1 nucleotides BWI76_RS02220 and BWI76_RS02225 overlap by 4 nucleotides
BWI76_RS02205: BWI76_RS02205 - phosphonate C-P lyase system protein PhnK, at 456,761 to 457,519
_RS02205
BWI76_RS02210: BWI76_RS02210 - carbon-phosphorus lyase complex subunit PhnJ, at 457,516 to 458,364
_RS02210
BWI76_RS02215: BWI76_RS02215 - carbon-phosphorus lyase complex subunit PhnI, at 458,357 to 459,421
_RS02215
BWI76_RS02220: BWI76_RS02220 - carbon-phosphorus lyase subunit PhnH, at 459,421 to 460,005
_RS02220
BWI76_RS02225: BWI76_RS02225 - phosphonate C-P lyase system protein PhnG, at 460,002 to 460,454
_RS02225
Position (kb) 458
459
460 Strain fitness (log2 ratio) -1
0
1
2 at 457.436 kb on + strand, within BWI76_RS02205 at 457.473 kb on + strand at 457.637 kb on + strand, within BWI76_RS02210 at 457.654 kb on + strand, within BWI76_RS02210 at 457.701 kb on + strand at 457.718 kb on + strand, within BWI76_RS02210 at 457.752 kb on + strand, within BWI76_RS02210 at 457.760 kb on - strand, within BWI76_RS02210 at 457.826 kb on + strand, within BWI76_RS02210 at 457.854 kb on - strand, within BWI76_RS02210 at 457.854 kb on - strand, within BWI76_RS02210 at 457.854 kb on - strand, within BWI76_RS02210 at 457.925 kb on - strand, within BWI76_RS02210 at 457.974 kb on + strand, within BWI76_RS02210 at 458.223 kb on - strand, within BWI76_RS02210 at 458.385 kb on + strand at 458.385 kb on + strand at 458.393 kb on - strand at 458.393 kb on - strand at 458.393 kb on - strand at 458.523 kb on - strand, within BWI76_RS02215 at 458.601 kb on - strand, within BWI76_RS02215 at 458.670 kb on + strand, within BWI76_RS02215 at 458.775 kb on - strand, within BWI76_RS02215 at 458.788 kb on + strand, within BWI76_RS02215 at 458.793 kb on + strand, within BWI76_RS02215 at 458.796 kb on - strand, within BWI76_RS02215 at 458.796 kb on - strand, within BWI76_RS02215 at 458.801 kb on - strand, within BWI76_RS02215 at 458.801 kb on - strand, within BWI76_RS02215 at 458.864 kb on + strand, within BWI76_RS02215 at 458.872 kb on - strand, within BWI76_RS02215 at 459.224 kb on - strand, within BWI76_RS02215 at 459.427 kb on - strand at 459.794 kb on + strand, within BWI76_RS02220 at 459.804 kb on + strand, within BWI76_RS02220 at 459.906 kb on + strand, within BWI76_RS02220 at 460.008 kb on - strand at 460.181 kb on - strand, within BWI76_RS02225 at 460.182 kb on - strand, within BWI76_RS02225 at 460.319 kb on + strand, within BWI76_RS02225 at 460.342 kb on + strand, within BWI76_RS02225
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 457,436 + BWI76_RS02205 0.89 +0.4 457,473 + -0.7 457,637 + BWI76_RS02210 0.14 +0.2 457,654 + BWI76_RS02210 0.16 -0.1 457,701 + +0.7 457,718 + BWI76_RS02210 0.24 +0.3 457,752 + BWI76_RS02210 0.28 +1.5 457,760 - BWI76_RS02210 0.29 -0.7 457,826 + BWI76_RS02210 0.37 +0.1 457,854 - BWI76_RS02210 0.40 +0.1 457,854 - BWI76_RS02210 0.40 -0.1 457,854 - BWI76_RS02210 0.40 +2.3 457,925 - BWI76_RS02210 0.48 -0.1 457,974 + BWI76_RS02210 0.54 +0.2 458,223 - BWI76_RS02210 0.83 +0.5 458,385 + +2.0 458,385 + -0.9 458,393 - +0.1 458,393 - +2.7 458,393 - +0.8 458,523 - BWI76_RS02215 0.16 -0.4 458,601 - BWI76_RS02215 0.23 -1.3 458,670 + BWI76_RS02215 0.29 -0.5 458,775 - BWI76_RS02215 0.39 -0.3 458,788 + BWI76_RS02215 0.40 -0.1 458,793 + BWI76_RS02215 0.41 +0.1 458,796 - BWI76_RS02215 0.41 -0.3 458,796 - BWI76_RS02215 0.41 +0.2 458,801 - BWI76_RS02215 0.42 +0.7 458,801 - BWI76_RS02215 0.42 -1.4 458,864 + BWI76_RS02215 0.48 -0.6 458,872 - BWI76_RS02215 0.48 +0.5 459,224 - BWI76_RS02215 0.81 -1.3 459,427 - +0.4 459,794 + BWI76_RS02220 0.64 +0.1 459,804 + BWI76_RS02220 0.65 -0.9 459,906 + BWI76_RS02220 0.83 +0.7 460,008 - -0.2 460,181 - BWI76_RS02225 0.40 +0.2 460,182 - BWI76_RS02225 0.40 -1.1 460,319 + BWI76_RS02225 0.70 -1.5 460,342 + BWI76_RS02225 0.75 +1.9
Or see this region's nucleotide sequence