Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16865

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16860 and BWI76_RS16865 are separated by 53 nucleotidesBWI76_RS16865 and BWI76_RS16870 are separated by 145 nucleotides BWI76_RS16860: BWI76_RS16860 - acyl-CoA dehydrogenase, at 3,496,352 to 3,497,440 _RS16860 BWI76_RS16865: BWI76_RS16865 - coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase-related flavin-dependent oxidoreductase, at 3,497,494 to 3,498,579 _RS16865 BWI76_RS16870: BWI76_RS16870 - LysR family transcriptional regulator, at 3,498,725 to 3,499,618 _RS16870 Position (kb) 3497 3498 3499Strain fitness (log2 ratio) -1 0 1at 3496.666 kb on + strand, within BWI76_RS16860at 3496.720 kb on - strand, within BWI76_RS16860at 3497.655 kb on + strand, within BWI76_RS16865at 3497.663 kb on + strand, within BWI76_RS16865at 3497.663 kb on - strand, within BWI76_RS16865at 3497.738 kb on + strand, within BWI76_RS16865at 3498.029 kb on + strand, within BWI76_RS16865at 3498.154 kb on + strand, within BWI76_RS16865at 3498.173 kb on + strandat 3498.181 kb on - strand, within BWI76_RS16865at 3498.647 kb on - strandat 3498.975 kb on + strand, within BWI76_RS16870at 3498.976 kb on - strand, within BWI76_RS16870at 3499.376 kb on - strand, within BWI76_RS16870

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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3,496,666 + BWI76_RS16860 0.29 +0.0
3,496,720 - BWI76_RS16860 0.34 +0.5
3,497,655 + BWI76_RS16865 0.15 -1.2
3,497,663 + BWI76_RS16865 0.16 -0.1
3,497,663 - BWI76_RS16865 0.16 -0.4
3,497,738 + BWI76_RS16865 0.22 -0.4
3,498,029 + BWI76_RS16865 0.49 -0.0
3,498,154 + BWI76_RS16865 0.61 -0.8
3,498,173 + +1.4
3,498,181 - BWI76_RS16865 0.63 -0.5
3,498,647 - -0.4
3,498,975 + BWI76_RS16870 0.28 +1.0
3,498,976 - BWI76_RS16870 0.28 +0.3
3,499,376 - BWI76_RS16870 0.73 +0.3

Or see this region's nucleotide sequence