Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13000 and BWI76_RS13005 are separated by 358 nucleotides BWI76_RS13005 and BWI76_RS13010 overlap by 4 nucleotides
BWI76_RS13000: BWI76_RS13000 - hypothetical protein, at 2,711,638 to 2,712,438
_RS13000
BWI76_RS13005: BWI76_RS13005 - thiamine pyrophosphate protein TPP-binding protein, at 2,712,797 to 2,714,491
_RS13005
BWI76_RS13010: BWI76_RS13010 - LysR family transcriptional regulator, at 2,714,488 to 2,715,408
_RS13010
Position (kb)
2712
2713
2714
2715 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2711.891 kb on - strand, within BWI76_RS13000 at 2711.898 kb on - strand, within BWI76_RS13000 at 2711.954 kb on + strand, within BWI76_RS13000 at 2712.277 kb on - strand, within BWI76_RS13000 at 2712.318 kb on - strand, within BWI76_RS13000 at 2712.346 kb on + strand, within BWI76_RS13000 at 2712.354 kb on - strand, within BWI76_RS13000 at 2712.387 kb on + strand at 2712.467 kb on + strand at 2712.467 kb on + strand at 2712.475 kb on - strand at 2712.475 kb on - strand at 2712.484 kb on - strand at 2712.557 kb on + strand at 2712.559 kb on + strand at 2712.562 kb on + strand at 2712.562 kb on + strand at 2712.562 kb on + strand at 2712.562 kb on + strand at 2712.562 kb on + strand at 2712.565 kb on - strand at 2712.570 kb on - strand at 2712.570 kb on - strand at 2712.570 kb on - strand at 2712.627 kb on + strand at 2712.635 kb on - strand at 2712.640 kb on + strand at 2712.648 kb on - strand at 2712.674 kb on + strand at 2712.682 kb on - strand at 2712.687 kb on + strand at 2712.695 kb on - strand at 2712.695 kb on - strand at 2712.695 kb on - strand at 2712.695 kb on - strand at 2712.968 kb on + strand, within BWI76_RS13005 at 2713.005 kb on + strand, within BWI76_RS13005 at 2713.122 kb on + strand, within BWI76_RS13005 at 2713.168 kb on + strand, within BWI76_RS13005 at 2713.218 kb on + strand, within BWI76_RS13005 at 2713.226 kb on - strand, within BWI76_RS13005 at 2713.560 kb on - strand, within BWI76_RS13005 at 2713.616 kb on + strand, within BWI76_RS13005 at 2713.624 kb on - strand, within BWI76_RS13005 at 2713.646 kb on - strand, within BWI76_RS13005 at 2713.694 kb on + strand, within BWI76_RS13005 at 2713.694 kb on + strand, within BWI76_RS13005 at 2713.703 kb on - strand at 2713.712 kb on - strand, within BWI76_RS13005 at 2713.712 kb on - strand, within BWI76_RS13005 at 2713.713 kb on - strand, within BWI76_RS13005 at 2713.713 kb on - strand at 2713.758 kb on - strand, within BWI76_RS13005 at 2713.760 kb on + strand, within BWI76_RS13005 at 2713.766 kb on - strand, within BWI76_RS13005 at 2713.915 kb on + strand, within BWI76_RS13005 at 2714.090 kb on + strand, within BWI76_RS13005 at 2714.150 kb on + strand, within BWI76_RS13005 at 2714.182 kb on + strand at 2714.187 kb on + strand, within BWI76_RS13005 at 2714.187 kb on + strand, within BWI76_RS13005 at 2714.189 kb on + strand, within BWI76_RS13005 at 2714.190 kb on - strand, within BWI76_RS13005 at 2714.501 kb on + strand at 2714.501 kb on + strand at 2714.815 kb on + strand, within BWI76_RS13010 at 2714.843 kb on - strand, within BWI76_RS13010 at 2715.004 kb on + strand, within BWI76_RS13010 at 2715.092 kb on - strand, within BWI76_RS13010 at 2715.184 kb on + strand, within BWI76_RS13010 at 2715.237 kb on - strand, within BWI76_RS13010 at 2715.401 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 2,711,891 - BWI76_RS13000 0.32 -1.1 2,711,898 - BWI76_RS13000 0.32 +0.2 2,711,954 + BWI76_RS13000 0.39 +0.7 2,712,277 - BWI76_RS13000 0.80 +4.4 2,712,318 - BWI76_RS13000 0.85 +1.6 2,712,346 + BWI76_RS13000 0.88 -0.1 2,712,354 - BWI76_RS13000 0.89 -1.8 2,712,387 + +0.0 2,712,467 + -0.7 2,712,467 + -0.2 2,712,475 - -1.7 2,712,475 - +0.9 2,712,484 - +1.0 2,712,557 + -0.3 2,712,559 + -1.5 2,712,562 + +0.0 2,712,562 + -0.8 2,712,562 + +0.2 2,712,562 + -0.1 2,712,562 + +0.5 2,712,565 - +0.4 2,712,570 - -1.0 2,712,570 - -0.0 2,712,570 - -0.6 2,712,627 + -0.2 2,712,635 - +0.4 2,712,640 + +0.1 2,712,648 - -0.1 2,712,674 + +0.3 2,712,682 - +0.2 2,712,687 + -0.4 2,712,695 - +0.4 2,712,695 - +0.3 2,712,695 - +0.4 2,712,695 - +0.3 2,712,968 + BWI76_RS13005 0.10 -0.1 2,713,005 + BWI76_RS13005 0.12 +0.4 2,713,122 + BWI76_RS13005 0.19 +0.7 2,713,168 + BWI76_RS13005 0.22 -0.8 2,713,218 + BWI76_RS13005 0.25 -1.1 2,713,226 - BWI76_RS13005 0.25 -0.8 2,713,560 - BWI76_RS13005 0.45 -0.5 2,713,616 + BWI76_RS13005 0.48 +0.4 2,713,624 - BWI76_RS13005 0.49 +0.3 2,713,646 - BWI76_RS13005 0.50 -0.3 2,713,694 + BWI76_RS13005 0.53 -2.4 2,713,694 + BWI76_RS13005 0.53 -0.9 2,713,703 - +0.9 2,713,712 - BWI76_RS13005 0.54 +1.1 2,713,712 - BWI76_RS13005 0.54 +0.3 2,713,713 - BWI76_RS13005 0.54 -0.1 2,713,713 - -0.6 2,713,758 - BWI76_RS13005 0.57 +0.2 2,713,760 + BWI76_RS13005 0.57 +0.0 2,713,766 - BWI76_RS13005 0.57 -1.2 2,713,915 + BWI76_RS13005 0.66 -0.6 2,714,090 + BWI76_RS13005 0.76 +0.5 2,714,150 + BWI76_RS13005 0.80 -0.1 2,714,182 + +0.0 2,714,187 + BWI76_RS13005 0.82 -1.6 2,714,187 + BWI76_RS13005 0.82 +0.5 2,714,189 + BWI76_RS13005 0.82 -0.6 2,714,190 - BWI76_RS13005 0.82 +0.1 2,714,501 + -0.2 2,714,501 + -0.0 2,714,815 + BWI76_RS13010 0.36 +1.2 2,714,843 - BWI76_RS13010 0.39 +0.5 2,715,004 + BWI76_RS13010 0.56 +0.5 2,715,092 - BWI76_RS13010 0.66 +0.5 2,715,184 + BWI76_RS13010 0.76 -0.2 2,715,237 - BWI76_RS13010 0.81 +2.5 2,715,401 + +0.4
Or see this region's nucleotide sequence