Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13005

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13000 and BWI76_RS13005 are separated by 358 nucleotidesBWI76_RS13005 and BWI76_RS13010 overlap by 4 nucleotides BWI76_RS13000: BWI76_RS13000 - hypothetical protein, at 2,711,638 to 2,712,438 _RS13000 BWI76_RS13005: BWI76_RS13005 - thiamine pyrophosphate protein TPP-binding protein, at 2,712,797 to 2,714,491 _RS13005 BWI76_RS13010: BWI76_RS13010 - LysR family transcriptional regulator, at 2,714,488 to 2,715,408 _RS13010 Position (kb) 2712 2713 2714 2715Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2711.891 kb on - strand, within BWI76_RS13000at 2711.898 kb on - strand, within BWI76_RS13000at 2711.954 kb on + strand, within BWI76_RS13000at 2712.277 kb on - strand, within BWI76_RS13000at 2712.318 kb on - strand, within BWI76_RS13000at 2712.346 kb on + strand, within BWI76_RS13000at 2712.354 kb on - strand, within BWI76_RS13000at 2712.387 kb on + strandat 2712.467 kb on + strandat 2712.467 kb on + strandat 2712.475 kb on - strandat 2712.475 kb on - strandat 2712.484 kb on - strandat 2712.557 kb on + strandat 2712.559 kb on + strandat 2712.562 kb on + strandat 2712.562 kb on + strandat 2712.562 kb on + strandat 2712.562 kb on + strandat 2712.562 kb on + strandat 2712.565 kb on - strandat 2712.570 kb on - strandat 2712.570 kb on - strandat 2712.570 kb on - strandat 2712.627 kb on + strandat 2712.635 kb on - strandat 2712.640 kb on + strandat 2712.648 kb on - strandat 2712.674 kb on + strandat 2712.682 kb on - strandat 2712.687 kb on + strandat 2712.695 kb on - strandat 2712.695 kb on - strandat 2712.695 kb on - strandat 2712.695 kb on - strandat 2712.968 kb on + strand, within BWI76_RS13005at 2713.005 kb on + strand, within BWI76_RS13005at 2713.122 kb on + strand, within BWI76_RS13005at 2713.168 kb on + strand, within BWI76_RS13005at 2713.218 kb on + strand, within BWI76_RS13005at 2713.226 kb on - strand, within BWI76_RS13005at 2713.560 kb on - strand, within BWI76_RS13005at 2713.616 kb on + strand, within BWI76_RS13005at 2713.624 kb on - strand, within BWI76_RS13005at 2713.646 kb on - strand, within BWI76_RS13005at 2713.694 kb on + strand, within BWI76_RS13005at 2713.694 kb on + strand, within BWI76_RS13005at 2713.703 kb on - strandat 2713.712 kb on - strand, within BWI76_RS13005at 2713.712 kb on - strand, within BWI76_RS13005at 2713.713 kb on - strand, within BWI76_RS13005at 2713.713 kb on - strandat 2713.758 kb on - strand, within BWI76_RS13005at 2713.760 kb on + strand, within BWI76_RS13005at 2713.766 kb on - strand, within BWI76_RS13005at 2713.915 kb on + strand, within BWI76_RS13005at 2714.090 kb on + strand, within BWI76_RS13005at 2714.150 kb on + strand, within BWI76_RS13005at 2714.182 kb on + strandat 2714.187 kb on + strand, within BWI76_RS13005at 2714.187 kb on + strand, within BWI76_RS13005at 2714.189 kb on + strand, within BWI76_RS13005at 2714.190 kb on - strand, within BWI76_RS13005at 2714.501 kb on + strandat 2714.501 kb on + strandat 2714.815 kb on + strand, within BWI76_RS13010at 2714.843 kb on - strand, within BWI76_RS13010at 2715.004 kb on + strand, within BWI76_RS13010at 2715.092 kb on - strand, within BWI76_RS13010at 2715.184 kb on + strand, within BWI76_RS13010at 2715.237 kb on - strand, within BWI76_RS13010at 2715.401 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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2,711,891 - BWI76_RS13000 0.32 -1.1
2,711,898 - BWI76_RS13000 0.32 +0.2
2,711,954 + BWI76_RS13000 0.39 +0.7
2,712,277 - BWI76_RS13000 0.80 +4.4
2,712,318 - BWI76_RS13000 0.85 +1.6
2,712,346 + BWI76_RS13000 0.88 -0.1
2,712,354 - BWI76_RS13000 0.89 -1.8
2,712,387 + +0.0
2,712,467 + -0.7
2,712,467 + -0.2
2,712,475 - -1.7
2,712,475 - +0.9
2,712,484 - +1.0
2,712,557 + -0.3
2,712,559 + -1.5
2,712,562 + +0.0
2,712,562 + -0.8
2,712,562 + +0.2
2,712,562 + -0.1
2,712,562 + +0.5
2,712,565 - +0.4
2,712,570 - -1.0
2,712,570 - -0.0
2,712,570 - -0.6
2,712,627 + -0.2
2,712,635 - +0.4
2,712,640 + +0.1
2,712,648 - -0.1
2,712,674 + +0.3
2,712,682 - +0.2
2,712,687 + -0.4
2,712,695 - +0.4
2,712,695 - +0.3
2,712,695 - +0.4
2,712,695 - +0.3
2,712,968 + BWI76_RS13005 0.10 -0.1
2,713,005 + BWI76_RS13005 0.12 +0.4
2,713,122 + BWI76_RS13005 0.19 +0.7
2,713,168 + BWI76_RS13005 0.22 -0.8
2,713,218 + BWI76_RS13005 0.25 -1.1
2,713,226 - BWI76_RS13005 0.25 -0.8
2,713,560 - BWI76_RS13005 0.45 -0.5
2,713,616 + BWI76_RS13005 0.48 +0.4
2,713,624 - BWI76_RS13005 0.49 +0.3
2,713,646 - BWI76_RS13005 0.50 -0.3
2,713,694 + BWI76_RS13005 0.53 -2.4
2,713,694 + BWI76_RS13005 0.53 -0.9
2,713,703 - +0.9
2,713,712 - BWI76_RS13005 0.54 +1.1
2,713,712 - BWI76_RS13005 0.54 +0.3
2,713,713 - BWI76_RS13005 0.54 -0.1
2,713,713 - -0.6
2,713,758 - BWI76_RS13005 0.57 +0.2
2,713,760 + BWI76_RS13005 0.57 +0.0
2,713,766 - BWI76_RS13005 0.57 -1.2
2,713,915 + BWI76_RS13005 0.66 -0.6
2,714,090 + BWI76_RS13005 0.76 +0.5
2,714,150 + BWI76_RS13005 0.80 -0.1
2,714,182 + +0.0
2,714,187 + BWI76_RS13005 0.82 -1.6
2,714,187 + BWI76_RS13005 0.82 +0.5
2,714,189 + BWI76_RS13005 0.82 -0.6
2,714,190 - BWI76_RS13005 0.82 +0.1
2,714,501 + -0.2
2,714,501 + -0.0
2,714,815 + BWI76_RS13010 0.36 +1.2
2,714,843 - BWI76_RS13010 0.39 +0.5
2,715,004 + BWI76_RS13010 0.56 +0.5
2,715,092 - BWI76_RS13010 0.66 +0.5
2,715,184 + BWI76_RS13010 0.76 -0.2
2,715,237 - BWI76_RS13010 0.81 +2.5
2,715,401 + +0.4

Or see this region's nucleotide sequence