Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04905 and BWI76_RS04910 are separated by 334 nucleotides BWI76_RS04910 and BWI76_RS04915 are separated by 175 nucleotides BWI76_RS04915 and BWI76_RS04920 are separated by 31 nucleotides
BWI76_RS04905: BWI76_RS04905 - DUF3300 domain-containing protein, at 1,027,508 to 1,029,094
_RS04905
BWI76_RS04910: BWI76_RS04910 - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase, at 1,029,429 to 1,032,047
_RS04910
BWI76_RS04915: BWI76_RS04915 - hypothetical protein, at 1,032,223 to 1,032,585
_RS04915
BWI76_RS04920: BWI76_RS04920 - S-adenosylmethionine decarboxylase, at 1,032,617 to 1,033,411
_RS04920
Position (kb)
1029
1030
1031
1032
1033 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 1028.712 kb on - strand, within BWI76_RS04905 at 1028.831 kb on - strand, within BWI76_RS04905 at 1028.846 kb on + strand, within BWI76_RS04905 at 1028.926 kb on - strand, within BWI76_RS04905 at 1028.926 kb on - strand, within BWI76_RS04905 at 1028.926 kb on - strand, within BWI76_RS04905 at 1028.962 kb on - strand at 1029.131 kb on + strand at 1029.241 kb on + strand at 1029.249 kb on - strand at 1029.276 kb on - strand at 1032.184 kb on + strand at 1032.320 kb on + strand, within BWI76_RS04915 at 1032.326 kb on + strand, within BWI76_RS04915 at 1032.367 kb on + strand, within BWI76_RS04915 at 1032.367 kb on + strand, within BWI76_RS04915 at 1032.436 kb on + strand, within BWI76_RS04915 at 1032.587 kb on - strand at 1032.614 kb on + strand at 1032.614 kb on + strand at 1032.686 kb on - strand at 1032.736 kb on - strand, within BWI76_RS04920 at 1032.744 kb on - strand, within BWI76_RS04920 at 1032.809 kb on + strand, within BWI76_RS04920 at 1032.842 kb on + strand, within BWI76_RS04920 at 1032.927 kb on - strand, within BWI76_RS04920 at 1032.995 kb on + strand, within BWI76_RS04920
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,028,712 - BWI76_RS04905 0.76 +0.0 1,028,831 - BWI76_RS04905 0.83 +1.2 1,028,846 + BWI76_RS04905 0.84 -0.9 1,028,926 - BWI76_RS04905 0.89 +0.7 1,028,926 - BWI76_RS04905 0.89 +0.2 1,028,926 - BWI76_RS04905 0.89 -1.6 1,028,962 - +0.1 1,029,131 + +1.5 1,029,241 + -0.7 1,029,249 - +2.0 1,029,276 - +0.1 1,032,184 + -0.0 1,032,320 + BWI76_RS04915 0.27 +0.0 1,032,326 + BWI76_RS04915 0.28 +0.4 1,032,367 + BWI76_RS04915 0.40 +0.2 1,032,367 + BWI76_RS04915 0.40 -1.6 1,032,436 + BWI76_RS04915 0.59 -0.4 1,032,587 - +0.4 1,032,614 + +1.6 1,032,614 + -0.5 1,032,686 - +1.0 1,032,736 - BWI76_RS04920 0.15 +1.2 1,032,744 - BWI76_RS04920 0.16 -0.4 1,032,809 + BWI76_RS04920 0.24 +0.3 1,032,842 + BWI76_RS04920 0.28 +0.4 1,032,927 - BWI76_RS04920 0.39 -0.9 1,032,995 + BWI76_RS04920 0.48 +5.6
Or see this region's nucleotide sequence