Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03185 and BWI76_RS03190 overlap by 4 nucleotides BWI76_RS03190 and BWI76_RS03195 overlap by 4 nucleotides BWI76_RS03195 and BWI76_RS03200 are separated by 11 nucleotides BWI76_RS03200 and BWI76_RS03205 are separated by 860 nucleotides
BWI76_RS03185: BWI76_RS03185 - CI repressor, at 656,609 to 657,166
_RS03185
BWI76_RS03190: BWI76_RS03190 - hypothetical protein, at 657,163 to 657,390
_RS03190
BWI76_RS03195: BWI76_RS03195 - P4 phage protein, at 657,387 to 657,707
_RS03195
BWI76_RS03200: BWI76_RS03200 - nucleoside triphosphatase, D5 family protein, at 657,719 to 660,052
_RS03200
BWI76_RS03205: BWI76_RS03205 - hypothetical protein, at 660,913 to 662,153
_RS03205
Position (kb)
657
658
659
660
661 Strain fitness (log2 ratio)
-1
0
1
2
3 at 656.784 kb on - strand, within BWI76_RS03185 at 656.802 kb on + strand, within BWI76_RS03185 at 656.868 kb on - strand, within BWI76_RS03185 at 656.960 kb on - strand, within BWI76_RS03185 at 656.994 kb on - strand, within BWI76_RS03185 at 657.258 kb on + strand, within BWI76_RS03190 at 657.260 kb on + strand, within BWI76_RS03190 at 657.330 kb on + strand, within BWI76_RS03190 at 657.416 kb on - strand at 657.435 kb on + strand, within BWI76_RS03195 at 657.453 kb on - strand, within BWI76_RS03195 at 657.476 kb on - strand, within BWI76_RS03195 at 657.884 kb on - strand at 657.931 kb on + strand at 658.035 kb on - strand, within BWI76_RS03200 at 658.107 kb on + strand, within BWI76_RS03200 at 658.294 kb on + strand, within BWI76_RS03200 at 658.302 kb on - strand, within BWI76_RS03200 at 658.391 kb on - strand, within BWI76_RS03200 at 658.425 kb on + strand, within BWI76_RS03200 at 658.781 kb on + strand, within BWI76_RS03200 at 658.840 kb on + strand, within BWI76_RS03200 at 658.884 kb on - strand, within BWI76_RS03200 at 658.904 kb on - strand, within BWI76_RS03200 at 658.943 kb on + strand, within BWI76_RS03200 at 658.949 kb on - strand, within BWI76_RS03200 at 659.278 kb on + strand, within BWI76_RS03200 at 659.516 kb on - strand, within BWI76_RS03200 at 659.653 kb on - strand, within BWI76_RS03200 at 659.683 kb on + strand, within BWI76_RS03200 at 659.779 kb on + strand, within BWI76_RS03200 at 659.907 kb on + strand at 659.907 kb on + strand at 660.056 kb on + strand at 660.106 kb on - strand at 660.267 kb on + strand at 660.267 kb on + strand at 660.284 kb on + strand at 660.292 kb on - strand at 660.292 kb on - strand at 660.735 kb on + strand at 660.788 kb on - strand at 660.824 kb on + strand at 660.839 kb on - strand at 660.839 kb on - strand at 660.875 kb on - strand at 660.875 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 656,784 - BWI76_RS03185 0.31 -0.5 656,802 + BWI76_RS03185 0.35 +0.3 656,868 - BWI76_RS03185 0.46 +2.0 656,960 - BWI76_RS03185 0.63 +1.4 656,994 - BWI76_RS03185 0.69 +1.3 657,258 + BWI76_RS03190 0.42 -0.2 657,260 + BWI76_RS03190 0.43 -0.3 657,330 + BWI76_RS03190 0.73 +0.1 657,416 - -0.4 657,435 + BWI76_RS03195 0.15 -0.7 657,453 - BWI76_RS03195 0.21 -0.0 657,476 - BWI76_RS03195 0.28 +0.0 657,884 - -0.7 657,931 + +0.0 658,035 - BWI76_RS03200 0.14 +0.2 658,107 + BWI76_RS03200 0.17 +2.4 658,294 + BWI76_RS03200 0.25 +0.5 658,302 - BWI76_RS03200 0.25 -0.4 658,391 - BWI76_RS03200 0.29 +0.1 658,425 + BWI76_RS03200 0.30 -0.3 658,781 + BWI76_RS03200 0.46 -0.0 658,840 + BWI76_RS03200 0.48 -1.0 658,884 - BWI76_RS03200 0.50 +3.4 658,904 - BWI76_RS03200 0.51 +0.4 658,943 + BWI76_RS03200 0.52 +0.7 658,949 - BWI76_RS03200 0.53 -0.9 659,278 + BWI76_RS03200 0.67 +2.7 659,516 - BWI76_RS03200 0.77 +0.9 659,653 - BWI76_RS03200 0.83 -1.2 659,683 + BWI76_RS03200 0.84 +3.3 659,779 + BWI76_RS03200 0.88 +1.6 659,907 + -1.0 659,907 + -1.2 660,056 + +0.0 660,106 - -0.3 660,267 + -0.5 660,267 + +1.1 660,284 + -0.3 660,292 - -0.6 660,292 - +2.9 660,735 + -1.1 660,788 - -0.5 660,824 + +0.1 660,839 - +0.3 660,839 - +1.5 660,875 - +0.4 660,875 - +0.0
Or see this region's nucleotide sequence