Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25240 and BWI76_RS25245 are separated by 14 nucleotides BWI76_RS25245 and BWI76_RS25250 overlap by 4 nucleotides BWI76_RS25250 and BWI76_RS25255 overlap by 32 nucleotides BWI76_RS25255 and BWI76_RS25260 are separated by 6 nucleotides
BWI76_RS25240: BWI76_RS25240 - D-arabinose 5-phosphate isomerase, at 5,248,414 to 5,249,400
_RS25240
BWI76_RS25245: BWI76_RS25245 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 5,249,415 to 5,249,981
_RS25245
BWI76_RS25250: BWI76_RS25250 - LPS export ABC transporter periplasmic protein LptC, at 5,249,978 to 5,250,553
_RS25250
BWI76_RS25255: BWI76_RS25255 - lipopolysaccharide ABC transporter substrate-binding protein LptA, at 5,250,522 to 5,251,067
_RS25255
BWI76_RS25260: BWI76_RS25260 - ABC transporter ATP-binding protein, at 5,251,074 to 5,251,799
_RS25260
Position (kb) 5249
5250
5251 Strain fitness (log2 ratio) -1
0
1 at 5249.215 kb on - strand, within BWI76_RS25240 at 5249.395 kb on + strand at 5249.446 kb on + strand at 5249.595 kb on + strand, within BWI76_RS25245 at 5249.720 kb on + strand, within BWI76_RS25245 at 5249.823 kb on + strand, within BWI76_RS25245 at 5249.945 kb on + strand at 5250.508 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 5,249,215 - BWI76_RS25240 0.81 +0.1 5,249,395 + +0.1 5,249,446 + -1.0 5,249,595 + BWI76_RS25245 0.32 -1.0 5,249,720 + BWI76_RS25245 0.54 -1.7 5,249,823 + BWI76_RS25245 0.72 -0.2 5,249,945 + -0.4 5,250,508 + -1.1
Or see this region's nucleotide sequence