Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15225

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15220 and BWI76_RS15225 are separated by 12 nucleotidesBWI76_RS15225 and BWI76_RS15230 are separated by 1 nucleotidesBWI76_RS15230 and BWI76_RS15235 overlap by 8 nucleotides BWI76_RS15220: BWI76_RS15220 - L-lactate dehydrogenase, at 3,190,094 to 3,191,038 _RS15220 BWI76_RS15225: BWI76_RS15225 - DeoR family transcriptional regulator, at 3,191,051 to 3,191,812 _RS15225 BWI76_RS15230: BWI76_RS15230 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 3,191,814 to 3,192,800 _RS15230 BWI76_RS15235: BWI76_RS15235 - hypothetical protein, at 3,192,793 to 3,194,031 _RS15235 Position (kb) 3191 3192Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3190.233 kb on + strand, within BWI76_RS15220at 3190.611 kb on + strand, within BWI76_RS15220at 3190.619 kb on - strand, within BWI76_RS15220at 3191.144 kb on + strand, within BWI76_RS15225at 3191.152 kb on - strand, within BWI76_RS15225at 3191.276 kb on - strand, within BWI76_RS15225at 3191.685 kb on + strand, within BWI76_RS15225at 3191.693 kb on - strand, within BWI76_RS15225at 3191.848 kb on - strandat 3191.851 kb on - strandat 3192.218 kb on + strand, within BWI76_RS15230at 3192.243 kb on - strand, within BWI76_RS15230at 3192.280 kb on + strand, within BWI76_RS15230at 3192.316 kb on + strand, within BWI76_RS15230at 3192.592 kb on + strand, within BWI76_RS15230at 3192.728 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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3,190,233 + BWI76_RS15220 0.15 +0.2
3,190,611 + BWI76_RS15220 0.55 -1.8
3,190,619 - BWI76_RS15220 0.56 -0.4
3,191,144 + BWI76_RS15225 0.12 -1.5
3,191,152 - BWI76_RS15225 0.13 -3.8
3,191,276 - BWI76_RS15225 0.30 -1.4
3,191,685 + BWI76_RS15225 0.83 -3.3
3,191,693 - BWI76_RS15225 0.84 -4.8
3,191,848 - -0.7
3,191,851 - -0.0
3,192,218 + BWI76_RS15230 0.41 -1.8
3,192,243 - BWI76_RS15230 0.43 -0.5
3,192,280 + BWI76_RS15230 0.47 +0.2
3,192,316 + BWI76_RS15230 0.51 +0.9
3,192,592 + BWI76_RS15230 0.79 +1.0
3,192,728 - -1.1

Or see this region's nucleotide sequence