Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12830 and BWI76_RS12835 are separated by 23 nucleotides BWI76_RS12835 and BWI76_RS12840 are separated by 27 nucleotides BWI76_RS12840 and BWI76_RS12845 are separated by 486 nucleotides
BWI76_RS12830: BWI76_RS12830 - flagellar motor switch protein FliY, at 2,673,624 to 2,674,454
_RS12830
BWI76_RS12835: BWI76_RS12835 - restriction endonuclease subunit S, at 2,674,478 to 2,675,350
_RS12835
BWI76_RS12840: BWI76_RS12840 - amino acid ABC transporter permease/ATP-binding protein, at 2,675,378 to 2,676,898
_RS12840
BWI76_RS12845: BWI76_RS12845 - LysR family transcriptional regulator, at 2,677,385 to 2,678,302
_RS12845
Position (kb)
2675
2676
2677 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7 at 2674.382 kb on - strand at 2674.421 kb on + strand at 2674.667 kb on - strand, within BWI76_RS12835 at 2674.678 kb on - strand, within BWI76_RS12835 at 2674.855 kb on - strand, within BWI76_RS12835 at 2674.992 kb on - strand, within BWI76_RS12835 at 2675.088 kb on - strand, within BWI76_RS12835 at 2675.189 kb on + strand, within BWI76_RS12835 at 2675.572 kb on - strand, within BWI76_RS12840 at 2675.688 kb on + strand, within BWI76_RS12840 at 2675.688 kb on + strand, within BWI76_RS12840 at 2675.816 kb on + strand, within BWI76_RS12840 at 2675.977 kb on + strand, within BWI76_RS12840 at 2675.977 kb on + strand, within BWI76_RS12840 at 2676.009 kb on + strand, within BWI76_RS12840 at 2676.312 kb on + strand, within BWI76_RS12840 at 2676.320 kb on - strand, within BWI76_RS12840 at 2676.587 kb on + strand, within BWI76_RS12840 at 2676.587 kb on + strand, within BWI76_RS12840 at 2676.595 kb on - strand, within BWI76_RS12840 at 2676.612 kb on + strand at 2676.620 kb on - strand, within BWI76_RS12840 at 2676.665 kb on + strand at 2676.702 kb on - strand, within BWI76_RS12840 at 2676.743 kb on - strand at 2676.787 kb on + strand at 2676.794 kb on + strand at 2676.801 kb on + strand at 2676.837 kb on - strand at 2676.839 kb on - strand at 2676.839 kb on - strand at 2676.866 kb on - strand at 2676.871 kb on - strand at 2676.876 kb on - strand at 2676.904 kb on + strand at 2676.946 kb on + strand at 2676.946 kb on + strand at 2676.968 kb on + strand at 2676.976 kb on - strand at 2676.976 kb on - strand at 2676.981 kb on - strand at 2676.982 kb on - strand at 2676.984 kb on + strand at 2676.985 kb on + strand at 2676.985 kb on + strand at 2676.985 kb on + strand at 2676.985 kb on + strand at 2676.985 kb on + strand at 2676.985 kb on + strand at 2676.987 kb on - strand at 2676.987 kb on - strand at 2676.988 kb on - strand at 2676.988 kb on - strand at 2676.993 kb on - strand at 2676.994 kb on - strand at 2676.999 kb on - strand at 2676.999 kb on - strand at 2677.001 kb on + strand at 2677.007 kb on - strand at 2677.169 kb on - strand at 2677.171 kb on + strand at 2677.198 kb on + strand at 2677.204 kb on + strand at 2677.211 kb on + strand at 2677.219 kb on - strand at 2677.293 kb on - strand at 2677.293 kb on - strand at 2677.470 kb on + strand at 2677.491 kb on + strand, within BWI76_RS12845 at 2677.563 kb on - strand, within BWI76_RS12845 at 2677.565 kb on - strand, within BWI76_RS12845 at 2677.565 kb on - strand, within BWI76_RS12845 at 2677.570 kb on - strand, within BWI76_RS12845 at 2677.768 kb on - strand, within BWI76_RS12845 at 2677.800 kb on - strand, within BWI76_RS12845 at 2677.800 kb on - strand, within BWI76_RS12845 at 2677.849 kb on + strand at 2677.873 kb on + strand at 2677.891 kb on + strand, within BWI76_RS12845 at 2677.891 kb on + strand, within BWI76_RS12845 at 2677.891 kb on + strand at 2677.891 kb on + strand, within BWI76_RS12845 at 2677.891 kb on + strand, within BWI76_RS12845 at 2677.891 kb on + strand, within BWI76_RS12845 at 2677.891 kb on + strand, within BWI76_RS12845 at 2677.891 kb on + strand, within BWI76_RS12845 at 2677.891 kb on + strand, within BWI76_RS12845
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 2,674,382 - +0.7 2,674,421 + +0.4 2,674,667 - BWI76_RS12835 0.22 -0.6 2,674,678 - BWI76_RS12835 0.23 -0.1 2,674,855 - BWI76_RS12835 0.43 -0.5 2,674,992 - BWI76_RS12835 0.59 -2.2 2,675,088 - BWI76_RS12835 0.70 +0.9 2,675,189 + BWI76_RS12835 0.81 -0.7 2,675,572 - BWI76_RS12840 0.13 +0.3 2,675,688 + BWI76_RS12840 0.20 -0.2 2,675,688 + BWI76_RS12840 0.20 +7.3 2,675,816 + BWI76_RS12840 0.29 -0.7 2,675,977 + BWI76_RS12840 0.39 -0.2 2,675,977 + BWI76_RS12840 0.39 -2.6 2,676,009 + BWI76_RS12840 0.41 +1.1 2,676,312 + BWI76_RS12840 0.61 +0.7 2,676,320 - BWI76_RS12840 0.62 -0.1 2,676,587 + BWI76_RS12840 0.79 +0.4 2,676,587 + BWI76_RS12840 0.79 +0.4 2,676,595 - BWI76_RS12840 0.80 -0.5 2,676,612 + +0.6 2,676,620 - BWI76_RS12840 0.82 +0.5 2,676,665 + +1.2 2,676,702 - BWI76_RS12840 0.87 +2.8 2,676,743 - +0.7 2,676,787 + -0.5 2,676,794 + -0.5 2,676,801 + +0.3 2,676,837 - -1.6 2,676,839 - -0.9 2,676,839 - -0.2 2,676,866 - +6.8 2,676,871 - +0.3 2,676,876 - +0.0 2,676,904 + -0.1 2,676,946 + -1.2 2,676,946 + -0.6 2,676,968 + +0.8 2,676,976 - -0.6 2,676,976 - -0.2 2,676,981 - +1.2 2,676,982 - +0.9 2,676,984 + -1.3 2,676,985 + +0.2 2,676,985 + -0.6 2,676,985 + -0.1 2,676,985 + +0.7 2,676,985 + +2.1 2,676,985 + -0.4 2,676,987 - +0.4 2,676,987 - -1.1 2,676,988 - -2.2 2,676,988 - +1.2 2,676,993 - -0.3 2,676,994 - +0.9 2,676,999 - -0.3 2,676,999 - +0.0 2,677,001 + -0.7 2,677,007 - +0.5 2,677,169 - -1.8 2,677,171 + +0.5 2,677,198 + -1.1 2,677,204 + +1.6 2,677,211 + +1.6 2,677,219 - -0.4 2,677,293 - +0.5 2,677,293 - +0.1 2,677,470 + +2.5 2,677,491 + BWI76_RS12845 0.12 +0.5 2,677,563 - BWI76_RS12845 0.19 -1.6 2,677,565 - BWI76_RS12845 0.20 +0.6 2,677,565 - BWI76_RS12845 0.20 +0.8 2,677,570 - BWI76_RS12845 0.20 +1.2 2,677,768 - BWI76_RS12845 0.42 +0.7 2,677,800 - BWI76_RS12845 0.45 -0.6 2,677,800 - BWI76_RS12845 0.45 -0.2 2,677,849 + +1.7 2,677,873 + +1.2 2,677,891 + BWI76_RS12845 0.55 -1.9 2,677,891 + BWI76_RS12845 0.55 -1.3 2,677,891 + -0.6 2,677,891 + BWI76_RS12845 0.55 +0.2 2,677,891 + BWI76_RS12845 0.55 +0.3 2,677,891 + BWI76_RS12845 0.55 -1.4 2,677,891 + BWI76_RS12845 0.55 -0.2 2,677,891 + BWI76_RS12845 0.55 +0.5 2,677,891 + BWI76_RS12845 0.55 -0.4
Or see this region's nucleotide sequence