Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12840

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12830 and BWI76_RS12835 are separated by 23 nucleotidesBWI76_RS12835 and BWI76_RS12840 are separated by 27 nucleotidesBWI76_RS12840 and BWI76_RS12845 are separated by 486 nucleotides BWI76_RS12830: BWI76_RS12830 - flagellar motor switch protein FliY, at 2,673,624 to 2,674,454 _RS12830 BWI76_RS12835: BWI76_RS12835 - restriction endonuclease subunit S, at 2,674,478 to 2,675,350 _RS12835 BWI76_RS12840: BWI76_RS12840 - amino acid ABC transporter permease/ATP-binding protein, at 2,675,378 to 2,676,898 _RS12840 BWI76_RS12845: BWI76_RS12845 - LysR family transcriptional regulator, at 2,677,385 to 2,678,302 _RS12845 Position (kb) 2675 2676 2677Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6 7at 2674.382 kb on - strandat 2674.421 kb on + strandat 2674.667 kb on - strand, within BWI76_RS12835at 2674.678 kb on - strand, within BWI76_RS12835at 2674.855 kb on - strand, within BWI76_RS12835at 2674.992 kb on - strand, within BWI76_RS12835at 2675.088 kb on - strand, within BWI76_RS12835at 2675.189 kb on + strand, within BWI76_RS12835at 2675.572 kb on - strand, within BWI76_RS12840at 2675.688 kb on + strand, within BWI76_RS12840at 2675.688 kb on + strand, within BWI76_RS12840at 2675.816 kb on + strand, within BWI76_RS12840at 2675.977 kb on + strand, within BWI76_RS12840at 2675.977 kb on + strand, within BWI76_RS12840at 2676.009 kb on + strand, within BWI76_RS12840at 2676.312 kb on + strand, within BWI76_RS12840at 2676.320 kb on - strand, within BWI76_RS12840at 2676.587 kb on + strand, within BWI76_RS12840at 2676.587 kb on + strand, within BWI76_RS12840at 2676.595 kb on - strand, within BWI76_RS12840at 2676.612 kb on + strandat 2676.620 kb on - strand, within BWI76_RS12840at 2676.665 kb on + strandat 2676.702 kb on - strand, within BWI76_RS12840at 2676.743 kb on - strandat 2676.787 kb on + strandat 2676.794 kb on + strandat 2676.801 kb on + strandat 2676.837 kb on - strandat 2676.839 kb on - strandat 2676.839 kb on - strandat 2676.866 kb on - strandat 2676.871 kb on - strandat 2676.876 kb on - strandat 2676.904 kb on + strandat 2676.946 kb on + strandat 2676.946 kb on + strandat 2676.968 kb on + strandat 2676.976 kb on - strandat 2676.976 kb on - strandat 2676.981 kb on - strandat 2676.982 kb on - strandat 2676.984 kb on + strandat 2676.985 kb on + strandat 2676.985 kb on + strandat 2676.985 kb on + strandat 2676.985 kb on + strandat 2676.985 kb on + strandat 2676.985 kb on + strandat 2676.987 kb on - strandat 2676.987 kb on - strandat 2676.988 kb on - strandat 2676.988 kb on - strandat 2676.993 kb on - strandat 2676.994 kb on - strandat 2676.999 kb on - strandat 2676.999 kb on - strandat 2677.001 kb on + strandat 2677.007 kb on - strandat 2677.169 kb on - strandat 2677.171 kb on + strandat 2677.198 kb on + strandat 2677.204 kb on + strandat 2677.211 kb on + strandat 2677.219 kb on - strandat 2677.293 kb on - strandat 2677.293 kb on - strandat 2677.470 kb on + strandat 2677.491 kb on + strand, within BWI76_RS12845at 2677.563 kb on - strand, within BWI76_RS12845at 2677.565 kb on - strand, within BWI76_RS12845at 2677.565 kb on - strand, within BWI76_RS12845at 2677.570 kb on - strand, within BWI76_RS12845at 2677.768 kb on - strand, within BWI76_RS12845at 2677.800 kb on - strand, within BWI76_RS12845at 2677.800 kb on - strand, within BWI76_RS12845at 2677.849 kb on + strandat 2677.873 kb on + strandat 2677.891 kb on + strand, within BWI76_RS12845at 2677.891 kb on + strand, within BWI76_RS12845at 2677.891 kb on + strandat 2677.891 kb on + strand, within BWI76_RS12845at 2677.891 kb on + strand, within BWI76_RS12845at 2677.891 kb on + strand, within BWI76_RS12845at 2677.891 kb on + strand, within BWI76_RS12845at 2677.891 kb on + strand, within BWI76_RS12845at 2677.891 kb on + strand, within BWI76_RS12845

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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2,674,382 - +0.7
2,674,421 + +0.4
2,674,667 - BWI76_RS12835 0.22 -0.6
2,674,678 - BWI76_RS12835 0.23 -0.1
2,674,855 - BWI76_RS12835 0.43 -0.5
2,674,992 - BWI76_RS12835 0.59 -2.2
2,675,088 - BWI76_RS12835 0.70 +0.9
2,675,189 + BWI76_RS12835 0.81 -0.7
2,675,572 - BWI76_RS12840 0.13 +0.3
2,675,688 + BWI76_RS12840 0.20 -0.2
2,675,688 + BWI76_RS12840 0.20 +7.3
2,675,816 + BWI76_RS12840 0.29 -0.7
2,675,977 + BWI76_RS12840 0.39 -0.2
2,675,977 + BWI76_RS12840 0.39 -2.6
2,676,009 + BWI76_RS12840 0.41 +1.1
2,676,312 + BWI76_RS12840 0.61 +0.7
2,676,320 - BWI76_RS12840 0.62 -0.1
2,676,587 + BWI76_RS12840 0.79 +0.4
2,676,587 + BWI76_RS12840 0.79 +0.4
2,676,595 - BWI76_RS12840 0.80 -0.5
2,676,612 + +0.6
2,676,620 - BWI76_RS12840 0.82 +0.5
2,676,665 + +1.2
2,676,702 - BWI76_RS12840 0.87 +2.8
2,676,743 - +0.7
2,676,787 + -0.5
2,676,794 + -0.5
2,676,801 + +0.3
2,676,837 - -1.6
2,676,839 - -0.9
2,676,839 - -0.2
2,676,866 - +6.8
2,676,871 - +0.3
2,676,876 - +0.0
2,676,904 + -0.1
2,676,946 + -1.2
2,676,946 + -0.6
2,676,968 + +0.8
2,676,976 - -0.6
2,676,976 - -0.2
2,676,981 - +1.2
2,676,982 - +0.9
2,676,984 + -1.3
2,676,985 + +0.2
2,676,985 + -0.6
2,676,985 + -0.1
2,676,985 + +0.7
2,676,985 + +2.1
2,676,985 + -0.4
2,676,987 - +0.4
2,676,987 - -1.1
2,676,988 - -2.2
2,676,988 - +1.2
2,676,993 - -0.3
2,676,994 - +0.9
2,676,999 - -0.3
2,676,999 - +0.0
2,677,001 + -0.7
2,677,007 - +0.5
2,677,169 - -1.8
2,677,171 + +0.5
2,677,198 + -1.1
2,677,204 + +1.6
2,677,211 + +1.6
2,677,219 - -0.4
2,677,293 - +0.5
2,677,293 - +0.1
2,677,470 + +2.5
2,677,491 + BWI76_RS12845 0.12 +0.5
2,677,563 - BWI76_RS12845 0.19 -1.6
2,677,565 - BWI76_RS12845 0.20 +0.6
2,677,565 - BWI76_RS12845 0.20 +0.8
2,677,570 - BWI76_RS12845 0.20 +1.2
2,677,768 - BWI76_RS12845 0.42 +0.7
2,677,800 - BWI76_RS12845 0.45 -0.6
2,677,800 - BWI76_RS12845 0.45 -0.2
2,677,849 + +1.7
2,677,873 + +1.2
2,677,891 + BWI76_RS12845 0.55 -1.9
2,677,891 + BWI76_RS12845 0.55 -1.3
2,677,891 + -0.6
2,677,891 + BWI76_RS12845 0.55 +0.2
2,677,891 + BWI76_RS12845 0.55 +0.3
2,677,891 + BWI76_RS12845 0.55 -1.4
2,677,891 + BWI76_RS12845 0.55 -0.2
2,677,891 + BWI76_RS12845 0.55 +0.5
2,677,891 + BWI76_RS12845 0.55 -0.4

Or see this region's nucleotide sequence