Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12460 and BWI76_RS12465 are separated by 19 nucleotides BWI76_RS12465 and BWI76_RS12470 are separated by 33 nucleotides BWI76_RS12470 and BWI76_RS12475 are separated by 100 nucleotides BWI76_RS12475 and BWI76_RS12480 overlap by 4 nucleotides
BWI76_RS12460: BWI76_RS12460 - envelope stress response membrane protein PspC, at 2,603,605 to 2,603,964
_RS12460
BWI76_RS12465: BWI76_RS12465 - phage shock protein D, at 2,603,984 to 2,604,214
_RS12465
BWI76_RS12470: BWI76_RS12470 - thiosulfate sulfurtransferase PspE, at 2,604,248 to 2,604,562
_RS12470
BWI76_RS12475: BWI76_RS12475 - hypothetical protein, at 2,604,663 to 2,606,060
_RS12475
BWI76_RS12480: BWI76_RS12480 - TIGR01620 family protein, at 2,606,057 to 2,607,118
_RS12480
Position (kb)
2604
2605
2606
2607 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 2603.698 kb on - strand, within BWI76_RS12460 at 2603.731 kb on - strand, within BWI76_RS12460 at 2604.075 kb on - strand, within BWI76_RS12465 at 2604.208 kb on + strand at 2604.307 kb on + strand, within BWI76_RS12470 at 2604.762 kb on + strand at 2604.764 kb on + strand at 2604.787 kb on - strand at 2604.787 kb on - strand at 2604.931 kb on - strand, within BWI76_RS12475 at 2605.111 kb on - strand, within BWI76_RS12475 at 2605.161 kb on - strand, within BWI76_RS12475 at 2605.223 kb on - strand, within BWI76_RS12475 at 2605.375 kb on + strand, within BWI76_RS12475 at 2605.385 kb on + strand, within BWI76_RS12475 at 2605.605 kb on - strand, within BWI76_RS12475 at 2605.670 kb on + strand, within BWI76_RS12475 at 2605.791 kb on - strand, within BWI76_RS12475 at 2605.869 kb on - strand, within BWI76_RS12475 at 2605.934 kb on - strand at 2606.225 kb on - strand, within BWI76_RS12480 at 2606.447 kb on - strand, within BWI76_RS12480 at 2606.567 kb on + strand, within BWI76_RS12480 at 2606.585 kb on - strand, within BWI76_RS12480 at 2606.804 kb on - strand, within BWI76_RS12480 at 2606.925 kb on + strand, within BWI76_RS12480
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 2,603,698 - BWI76_RS12460 0.26 -0.3 2,603,731 - BWI76_RS12460 0.35 +0.8 2,604,075 - BWI76_RS12465 0.39 +0.6 2,604,208 + +0.3 2,604,307 + BWI76_RS12470 0.19 -0.3 2,604,762 + -0.3 2,604,764 + -0.7 2,604,787 - -1.2 2,604,787 - -0.5 2,604,931 - BWI76_RS12475 0.19 +0.5 2,605,111 - BWI76_RS12475 0.32 -0.5 2,605,161 - BWI76_RS12475 0.36 -0.4 2,605,223 - BWI76_RS12475 0.40 -0.3 2,605,375 + BWI76_RS12475 0.51 +0.1 2,605,385 + BWI76_RS12475 0.52 +6.2 2,605,605 - BWI76_RS12475 0.67 -0.0 2,605,670 + BWI76_RS12475 0.72 +0.3 2,605,791 - BWI76_RS12475 0.81 -1.1 2,605,869 - BWI76_RS12475 0.86 +0.3 2,605,934 - +0.7 2,606,225 - BWI76_RS12480 0.16 +0.4 2,606,447 - BWI76_RS12480 0.37 -1.0 2,606,567 + BWI76_RS12480 0.48 +0.1 2,606,585 - BWI76_RS12480 0.50 +0.2 2,606,804 - BWI76_RS12480 0.70 +0.1 2,606,925 + BWI76_RS12480 0.82 +0.8
Or see this region's nucleotide sequence