Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05725 and BWI76_RS05730 are separated by 453 nucleotides BWI76_RS05730 and BWI76_RS05735 are separated by 99 nucleotides BWI76_RS05735 and BWI76_RS05740 overlap by 14 nucleotides BWI76_RS05740 and BWI76_RS05745 are separated by 143 nucleotides
BWI76_RS05725: BWI76_RS05725 - beta-ketoacyl-[acyl-carrier-protein] synthase II, at 1,204,721 to 1,206,007
_RS05725
BWI76_RS05730: BWI76_RS05730 - hypothetical protein, at 1,206,461 to 1,207,207
_RS05730
BWI76_RS05735: BWI76_RS05735 - transcriptional regulator, at 1,207,307 to 1,207,675
_RS05735
BWI76_RS05740: BWI76_RS05740 - hypothetical protein, at 1,207,662 to 1,207,898
_RS05740
BWI76_RS05745: BWI76_RS05745 - hypothetical protein, at 1,208,042 to 1,209,280
_RS05745
Position (kb)
1206
1207
1208 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 1205.533 kb on + strand, within BWI76_RS05725 at 1205.625 kb on - strand, within BWI76_RS05725 at 1205.625 kb on - strand, within BWI76_RS05725 at 1205.643 kb on - strand, within BWI76_RS05725 at 1205.684 kb on - strand, within BWI76_RS05725 at 1205.721 kb on - strand, within BWI76_RS05725 at 1205.723 kb on + strand, within BWI76_RS05725 at 1205.742 kb on - strand, within BWI76_RS05725 at 1205.775 kb on + strand, within BWI76_RS05725 at 1205.782 kb on + strand, within BWI76_RS05725 at 1205.809 kb on + strand, within BWI76_RS05725 at 1205.825 kb on + strand, within BWI76_RS05725 at 1205.838 kb on + strand, within BWI76_RS05725 at 1205.876 kb on - strand, within BWI76_RS05725 at 1205.885 kb on - strand at 1205.888 kb on + strand at 1205.888 kb on + strand at 1205.889 kb on + strand at 1205.897 kb on - strand at 1205.912 kb on - strand at 1205.916 kb on + strand at 1205.924 kb on + strand at 1205.924 kb on + strand at 1205.932 kb on - strand at 1205.932 kb on - strand at 1205.932 kb on - strand at 1205.932 kb on - strand at 1205.932 kb on - strand at 1205.932 kb on - strand at 1205.932 kb on - strand at 1205.949 kb on + strand at 1205.957 kb on - strand at 1205.957 kb on - strand at 1205.978 kb on + strand at 1206.021 kb on - strand at 1206.039 kb on + strand at 1206.047 kb on + strand at 1206.047 kb on - strand at 1206.049 kb on + strand at 1206.049 kb on + strand at 1206.049 kb on + strand at 1206.057 kb on - strand at 1206.092 kb on + strand at 1206.134 kb on + strand at 1206.145 kb on + strand at 1206.150 kb on - strand at 1206.152 kb on + strand at 1206.153 kb on - strand at 1206.170 kb on - strand at 1206.173 kb on - strand at 1206.180 kb on - strand at 1206.198 kb on + strand at 1206.208 kb on + strand at 1206.208 kb on + strand at 1206.208 kb on + strand at 1206.208 kb on + strand at 1206.208 kb on + strand at 1206.214 kb on - strand at 1206.214 kb on - strand at 1206.214 kb on - strand at 1206.216 kb on - strand at 1206.216 kb on - strand at 1206.216 kb on - strand at 1206.216 kb on - strand at 1206.216 kb on - strand at 1206.221 kb on - strand at 1206.270 kb on - strand at 1206.299 kb on - strand at 1206.303 kb on + strand at 1206.303 kb on + strand at 1206.311 kb on - strand at 1206.311 kb on - strand at 1206.311 kb on - strand at 1206.313 kb on + strand at 1206.329 kb on - strand at 1206.375 kb on - strand at 1206.402 kb on - strand at 1206.426 kb on + strand at 1206.426 kb on + strand at 1206.431 kb on + strand at 1206.434 kb on - strand at 1206.458 kb on + strand at 1206.458 kb on + strand at 1206.458 kb on + strand at 1206.458 kb on + strand at 1206.463 kb on - strand at 1206.466 kb on - strand at 1206.468 kb on + strand at 1206.471 kb on - strand at 1206.471 kb on - strand at 1206.518 kb on - strand at 1206.601 kb on + strand, within BWI76_RS05730 at 1206.601 kb on + strand, within BWI76_RS05730 at 1206.780 kb on + strand, within BWI76_RS05730 at 1206.780 kb on + strand, within BWI76_RS05730 at 1206.788 kb on - strand, within BWI76_RS05730 at 1206.893 kb on - strand, within BWI76_RS05730 at 1206.919 kb on + strand, within BWI76_RS05730 at 1206.919 kb on + strand, within BWI76_RS05730 at 1206.924 kb on + strand, within BWI76_RS05730 at 1206.995 kb on + strand, within BWI76_RS05730 at 1207.021 kb on + strand, within BWI76_RS05730 at 1207.021 kb on + strand, within BWI76_RS05730 at 1207.021 kb on + strand, within BWI76_RS05730 at 1207.029 kb on - strand, within BWI76_RS05730 at 1207.050 kb on + strand, within BWI76_RS05730 at 1207.050 kb on + strand, within BWI76_RS05730 at 1207.060 kb on - strand, within BWI76_RS05730 at 1207.101 kb on - strand, within BWI76_RS05730 at 1207.102 kb on + strand, within BWI76_RS05730 at 1207.110 kb on - strand, within BWI76_RS05730 at 1207.235 kb on + strand at 1207.241 kb on + strand at 1207.248 kb on + strand at 1207.256 kb on - strand at 1207.373 kb on + strand, within BWI76_RS05735 at 1207.449 kb on + strand, within BWI76_RS05735 at 1207.503 kb on + strand, within BWI76_RS05735 at 1207.503 kb on + strand, within BWI76_RS05735 at 1207.511 kb on - strand, within BWI76_RS05735 at 1207.551 kb on - strand, within BWI76_RS05735 at 1207.556 kb on + strand, within BWI76_RS05735 at 1207.566 kb on + strand, within BWI76_RS05735 at 1207.586 kb on + strand, within BWI76_RS05735 at 1207.588 kb on + strand, within BWI76_RS05735 at 1207.593 kb on + strand, within BWI76_RS05735 at 1207.598 kb on + strand, within BWI76_RS05735 at 1207.598 kb on + strand, within BWI76_RS05735 at 1207.598 kb on + strand, within BWI76_RS05735 at 1207.601 kb on - strand, within BWI76_RS05735 at 1207.603 kb on + strand, within BWI76_RS05735 at 1207.606 kb on - strand, within BWI76_RS05735 at 1207.618 kb on - strand, within BWI76_RS05735 at 1207.654 kb on + strand at 1207.659 kb on + strand at 1207.659 kb on + strand at 1207.667 kb on - strand at 1207.667 kb on - strand at 1207.667 kb on - strand at 1207.678 kb on - strand at 1207.701 kb on - strand, within BWI76_RS05740 at 1207.744 kb on + strand, within BWI76_RS05740 at 1207.768 kb on + strand, within BWI76_RS05740 at 1207.775 kb on + strand, within BWI76_RS05740 at 1207.775 kb on + strand, within BWI76_RS05740 at 1207.811 kb on - strand, within BWI76_RS05740 at 1207.839 kb on - strand, within BWI76_RS05740 at 1207.839 kb on - strand, within BWI76_RS05740 at 1208.008 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,205,533 + BWI76_RS05725 0.63 -1.9 1,205,625 - BWI76_RS05725 0.70 -0.1 1,205,625 - BWI76_RS05725 0.70 -0.7 1,205,643 - BWI76_RS05725 0.72 +0.1 1,205,684 - BWI76_RS05725 0.75 +1.1 1,205,721 - BWI76_RS05725 0.78 -0.5 1,205,723 + BWI76_RS05725 0.78 -0.2 1,205,742 - BWI76_RS05725 0.79 +1.8 1,205,775 + BWI76_RS05725 0.82 +0.7 1,205,782 + BWI76_RS05725 0.82 +1.8 1,205,809 + BWI76_RS05725 0.85 -0.3 1,205,825 + BWI76_RS05725 0.86 +2.3 1,205,838 + BWI76_RS05725 0.87 -0.5 1,205,876 - BWI76_RS05725 0.90 -1.9 1,205,885 - +0.8 1,205,888 + -0.7 1,205,888 + +0.0 1,205,889 + +0.7 1,205,897 - -0.1 1,205,912 - -2.0 1,205,916 + +2.0 1,205,924 + +0.0 1,205,924 + -1.2 1,205,932 - -0.9 1,205,932 - +1.0 1,205,932 - +3.0 1,205,932 - -0.4 1,205,932 - -1.2 1,205,932 - -0.6 1,205,932 - +0.1 1,205,949 + -1.8 1,205,957 - +0.2 1,205,957 - +0.2 1,205,978 + -0.8 1,206,021 - -1.0 1,206,039 + +0.0 1,206,047 + +0.3 1,206,047 - -0.5 1,206,049 + -0.3 1,206,049 + -1.0 1,206,049 + -0.6 1,206,057 - +1.1 1,206,092 + -0.6 1,206,134 + -0.6 1,206,145 + +0.1 1,206,150 - +0.6 1,206,152 + -0.8 1,206,153 - -1.3 1,206,170 - +0.7 1,206,173 - +0.9 1,206,180 - +0.2 1,206,198 + +0.0 1,206,208 + +0.3 1,206,208 + +1.8 1,206,208 + -0.2 1,206,208 + +1.8 1,206,208 + +0.3 1,206,214 - -0.8 1,206,214 - +0.9 1,206,214 - -2.2 1,206,216 - +0.2 1,206,216 - -0.5 1,206,216 - +0.8 1,206,216 - +0.0 1,206,216 - +0.7 1,206,221 - -0.6 1,206,270 - +0.6 1,206,299 - -1.0 1,206,303 + +6.5 1,206,303 + -1.0 1,206,311 - -0.4 1,206,311 - +0.5 1,206,311 - -0.7 1,206,313 + -0.0 1,206,329 - -0.4 1,206,375 - +0.2 1,206,402 - -0.6 1,206,426 + +0.3 1,206,426 + -0.5 1,206,431 + -0.5 1,206,434 - +0.3 1,206,458 + -0.5 1,206,458 + -0.2 1,206,458 + +0.4 1,206,458 + +0.2 1,206,463 - -0.0 1,206,466 - -1.3 1,206,468 + +0.0 1,206,471 - -0.1 1,206,471 - +0.1 1,206,518 - +0.2 1,206,601 + BWI76_RS05730 0.19 -0.0 1,206,601 + BWI76_RS05730 0.19 -0.4 1,206,780 + BWI76_RS05730 0.43 -1.5 1,206,780 + BWI76_RS05730 0.43 -1.2 1,206,788 - BWI76_RS05730 0.44 -0.2 1,206,893 - BWI76_RS05730 0.58 -1.4 1,206,919 + BWI76_RS05730 0.61 -1.0 1,206,919 + BWI76_RS05730 0.61 -0.1 1,206,924 + BWI76_RS05730 0.62 -1.2 1,206,995 + BWI76_RS05730 0.71 -0.4 1,207,021 + BWI76_RS05730 0.75 -0.5 1,207,021 + BWI76_RS05730 0.75 +0.7 1,207,021 + BWI76_RS05730 0.75 -0.2 1,207,029 - BWI76_RS05730 0.76 +0.6 1,207,050 + BWI76_RS05730 0.79 -0.5 1,207,050 + BWI76_RS05730 0.79 -0.4 1,207,060 - BWI76_RS05730 0.80 -0.8 1,207,101 - BWI76_RS05730 0.86 -1.3 1,207,102 + BWI76_RS05730 0.86 +0.2 1,207,110 - BWI76_RS05730 0.87 -1.4 1,207,235 + +0.0 1,207,241 + +1.8 1,207,248 + -0.6 1,207,256 - +0.1 1,207,373 + BWI76_RS05735 0.18 +0.3 1,207,449 + BWI76_RS05735 0.38 +0.6 1,207,503 + BWI76_RS05735 0.53 +1.2 1,207,503 + BWI76_RS05735 0.53 +1.1 1,207,511 - BWI76_RS05735 0.55 -0.8 1,207,551 - BWI76_RS05735 0.66 -1.4 1,207,556 + BWI76_RS05735 0.67 -1.0 1,207,566 + BWI76_RS05735 0.70 +1.0 1,207,586 + BWI76_RS05735 0.76 -1.0 1,207,588 + BWI76_RS05735 0.76 -1.0 1,207,593 + BWI76_RS05735 0.78 +0.8 1,207,598 + BWI76_RS05735 0.79 +0.5 1,207,598 + BWI76_RS05735 0.79 +1.8 1,207,598 + BWI76_RS05735 0.79 +0.4 1,207,601 - BWI76_RS05735 0.80 -1.4 1,207,603 + BWI76_RS05735 0.80 -0.7 1,207,606 - BWI76_RS05735 0.81 -0.1 1,207,618 - BWI76_RS05735 0.84 -0.9 1,207,654 + +0.6 1,207,659 + -0.7 1,207,659 + +0.5 1,207,667 - +1.0 1,207,667 - +0.2 1,207,667 - -1.4 1,207,678 - -0.2 1,207,701 - BWI76_RS05740 0.16 +0.1 1,207,744 + BWI76_RS05740 0.35 -0.3 1,207,768 + BWI76_RS05740 0.45 -0.6 1,207,775 + BWI76_RS05740 0.48 -0.4 1,207,775 + BWI76_RS05740 0.48 +0.7 1,207,811 - BWI76_RS05740 0.63 -1.2 1,207,839 - BWI76_RS05740 0.75 +0.7 1,207,839 - BWI76_RS05740 0.75 +0.0 1,208,008 + -0.3
Or see this region's nucleotide sequence