Experiment: Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26810 and BWI76_RS26815 are separated by 83 nucleotides BWI76_RS26815 and BWI76_RS26820 are separated by 351 nucleotides BWI76_RS26820 and BWI76_RS26825 overlap by 20 nucleotides BWI76_RS26825 and BWI76_RS26830 are separated by 48 nucleotides BWI76_RS26830 and BWI76_RS26835 are separated by 11 nucleotides BWI76_RS26835 and BWI76_RS26840 are separated by 2 nucleotides
BWI76_RS26810: BWI76_RS26810 - cellulose biosynthesis protein BcsG, at 5,558,766 to 5,560,442
_RS26810
BWI76_RS26815: BWI76_RS26815 - small toxic polypeptide ldrD (Modular protein), at 5,560,526 to 5,560,651
_RS26815
BWI76_RS26820: BWI76_RS26820 - small toxic polypeptide ldrD (Modular protein), at 5,561,003 to 5,561,128
_RS26820
BWI76_RS26825: BWI76_RS26825 - hypothetical protein, at 5,561,109 to 5,561,327
_RS26825
BWI76_RS26830: BWI76_RS26830 - endoglucanase, at 5,561,376 to 5,562,380
_RS26830
BWI76_RS26835: BWI76_RS26835 - hypothetical protein, at 5,562,392 to 5,562,865
_RS26835
BWI76_RS26840: BWI76_RS26840 - cellulose synthase, at 5,562,868 to 5,566,920
_RS26840
Position (kb)
5561
5562
5563 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5560.399 kb on + strand at 5560.492 kb on - strand at 5560.729 kb on - strand at 5560.964 kb on - strand at 5561.144 kb on + strand, within BWI76_RS26825 at 5561.342 kb on + strand at 5561.398 kb on + strand at 5561.477 kb on + strand, within BWI76_RS26830 at 5561.477 kb on + strand, within BWI76_RS26830 at 5561.544 kb on - strand, within BWI76_RS26830 at 5561.772 kb on + strand, within BWI76_RS26830 at 5561.829 kb on + strand, within BWI76_RS26830 at 5561.860 kb on + strand, within BWI76_RS26830 at 5561.868 kb on - strand, within BWI76_RS26830 at 5561.876 kb on - strand, within BWI76_RS26830 at 5562.152 kb on + strand, within BWI76_RS26830 at 5562.160 kb on - strand, within BWI76_RS26830 at 5562.324 kb on - strand at 5562.454 kb on - strand, within BWI76_RS26835 at 5562.471 kb on + strand, within BWI76_RS26835 at 5562.478 kb on - strand, within BWI76_RS26835 at 5562.484 kb on - strand, within BWI76_RS26835 at 5562.523 kb on + strand, within BWI76_RS26835 at 5562.604 kb on + strand, within BWI76_RS26835 at 5562.612 kb on - strand, within BWI76_RS26835 at 5562.612 kb on - strand, within BWI76_RS26835 at 5562.622 kb on - strand, within BWI76_RS26835 at 5562.657 kb on + strand, within BWI76_RS26835 at 5562.665 kb on - strand, within BWI76_RS26835 at 5562.969 kb on - strand at 5562.975 kb on - strand at 5563.055 kb on + strand at 5563.057 kb on + strand at 5563.119 kb on + strand at 5563.123 kb on - strand at 5563.142 kb on - strand at 5563.219 kb on + strand at 5563.355 kb on - strand, within BWI76_RS26840
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.2X remove 5,560,399 + -1.4 5,560,492 - -0.3 5,560,729 - +0.7 5,560,964 - -2.8 5,561,144 + BWI76_RS26825 0.16 +0.1 5,561,342 + -0.4 5,561,398 + +0.2 5,561,477 + BWI76_RS26830 0.10 +0.2 5,561,477 + BWI76_RS26830 0.10 +2.2 5,561,544 - BWI76_RS26830 0.17 +0.3 5,561,772 + BWI76_RS26830 0.39 +1.9 5,561,829 + BWI76_RS26830 0.45 +0.3 5,561,860 + BWI76_RS26830 0.48 -1.3 5,561,868 - BWI76_RS26830 0.49 +0.6 5,561,876 - BWI76_RS26830 0.50 -1.1 5,562,152 + BWI76_RS26830 0.77 +1.0 5,562,160 - BWI76_RS26830 0.78 +1.1 5,562,324 - -0.1 5,562,454 - BWI76_RS26835 0.13 +0.8 5,562,471 + BWI76_RS26835 0.17 -0.4 5,562,478 - BWI76_RS26835 0.18 -0.1 5,562,484 - BWI76_RS26835 0.19 +0.3 5,562,523 + BWI76_RS26835 0.28 +0.3 5,562,604 + BWI76_RS26835 0.45 +0.9 5,562,612 - BWI76_RS26835 0.46 +0.7 5,562,612 - BWI76_RS26835 0.46 -0.9 5,562,622 - BWI76_RS26835 0.49 +0.9 5,562,657 + BWI76_RS26835 0.56 +0.2 5,562,665 - BWI76_RS26835 0.58 -0.4 5,562,969 - -0.0 5,562,975 - +0.2 5,563,055 + +0.5 5,563,057 + +0.1 5,563,119 + -0.3 5,563,123 - +0.9 5,563,142 - -0.9 5,563,219 + +0.4 5,563,355 - BWI76_RS26840 0.12 +0.5
Or see this region's nucleotide sequence