Experiment: Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07080 and BWI76_RS07085 are separated by 3 nucleotides BWI76_RS07085 and BWI76_RS07090 are separated by 175 nucleotides BWI76_RS07090 and BWI76_RS07095 are separated by 137 nucleotides BWI76_RS07095 and BWI76_RS07100 overlap by 1 nucleotides
BWI76_RS07080: BWI76_RS07080 - UDP-2,3-diacylglucosamine diphosphatase, at 1,488,656 to 1,489,378
_RS07080
BWI76_RS07085: BWI76_RS07085 - peptidyl-prolyl cis-trans isomerase, at 1,489,382 to 1,489,876
_RS07085
BWI76_RS07090: BWI76_RS07090 - cysteine--tRNA ligase, at 1,490,052 to 1,491,437
_RS07090
BWI76_RS07095: BWI76_RS07095 - ribosome-associated protein, at 1,491,575 to 1,491,787
_RS07095
BWI76_RS07100: BWI76_RS07100 - bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, at 1,491,787 to 1,492,653
_RS07100
Position (kb) 1490
1491
1492 Strain fitness (log2 ratio) -2
-1
0
1 at 1489.529 kb on - strand, within BWI76_RS07085 at 1489.987 kb on - strand at 1491.487 kb on + strand at 1491.487 kb on + strand at 1491.586 kb on + strand at 1491.609 kb on - strand, within BWI76_RS07095 at 1491.674 kb on - strand, within BWI76_RS07095 at 1491.730 kb on - strand, within BWI76_RS07095 at 1491.731 kb on - strand, within BWI76_RS07095 at 1491.803 kb on - strand at 1491.813 kb on - strand at 1491.822 kb on + strand at 1491.822 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.2X remove 1,489,529 - BWI76_RS07085 0.30 -0.1 1,489,987 - +0.6 1,491,487 + +0.3 1,491,487 + -0.2 1,491,586 + -0.0 1,491,609 - BWI76_RS07095 0.16 -1.9 1,491,674 - BWI76_RS07095 0.46 -1.6 1,491,730 - BWI76_RS07095 0.73 -0.4 1,491,731 - BWI76_RS07095 0.73 -1.1 1,491,803 - +0.0 1,491,813 - -2.1 1,491,822 + -2.4 1,491,822 + -1.5
Or see this region's nucleotide sequence