Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS03025

Experiment: Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS03015 and BWI76_RS03020 are separated by 52 nucleotidesBWI76_RS03020 and BWI76_RS03025 are separated by 99 nucleotidesBWI76_RS03025 and BWI76_RS03030 are separated by 123 nucleotides BWI76_RS03015: BWI76_RS03015 - RNase E inhibitor protein, at 617,067 to 617,492 _RS03015 BWI76_RS03020: BWI76_RS03020 - tRNA hydroxylase, at 617,545 to 618,303 _RS03020 BWI76_RS03025: BWI76_RS03025 - N-acetyltransferase, at 618,403 to 618,906 _RS03025 BWI76_RS03030: BWI76_RS03030 - valine--tRNA ligase, at 619,030 to 621,885 _RS03030 Position (kb) 618 619Strain fitness (log2 ratio) -1 0 1at 617.607 kb on - strandat 617.654 kb on - strand, within BWI76_RS03020at 617.665 kb on - strand, within BWI76_RS03020at 617.665 kb on - strand, within BWI76_RS03020at 617.761 kb on - strand, within BWI76_RS03020at 617.995 kb on - strand, within BWI76_RS03020at 617.995 kb on - strand, within BWI76_RS03020at 618.331 kb on - strandat 618.410 kb on + strandat 618.565 kb on + strand, within BWI76_RS03025at 618.573 kb on - strand, within BWI76_RS03025at 618.605 kb on - strand, within BWI76_RS03025at 618.884 kb on - strandat 618.885 kb on - strandat 618.966 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
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617,607 - -0.7
617,654 - BWI76_RS03020 0.14 -0.1
617,665 - BWI76_RS03020 0.16 -0.8
617,665 - BWI76_RS03020 0.16 +0.4
617,761 - BWI76_RS03020 0.28 +0.0
617,995 - BWI76_RS03020 0.59 +0.6
617,995 - BWI76_RS03020 0.59 -0.9
618,331 - -0.5
618,410 + -0.0
618,565 + BWI76_RS03025 0.32 +0.2
618,573 - BWI76_RS03025 0.34 +1.4
618,605 - BWI76_RS03025 0.40 +1.1
618,884 - -0.5
618,885 - +0.2
618,966 + +0.5

Or see this region's nucleotide sequence