Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00865

Experiment: Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00855 and BWI76_RS00860 are separated by 31 nucleotidesBWI76_RS00860 and BWI76_RS00865 overlap by 1 nucleotidesBWI76_RS00865 and BWI76_RS00870 overlap by 18 nucleotides BWI76_RS00855: BWI76_RS00855 - argininosuccinate lyase, at 176,786 to 178,159 _RS00855 BWI76_RS00860: BWI76_RS00860 - hypothetical protein, at 178,191 to 178,430 _RS00860 BWI76_RS00865: BWI76_RS00865 - DNA-binding transcriptional regulator OxyR, at 178,430 to 179,347 _RS00865 BWI76_RS00870: BWI76_RS00870 - NAD(P)(+) transhydrogenase, at 179,330 to 180,730 _RS00870 Position (kb) 178 179 180Strain fitness (log2 ratio) -1 0 1 2at 177.458 kb on + strand, within BWI76_RS00855at 177.466 kb on - strand, within BWI76_RS00855at 177.598 kb on + strand, within BWI76_RS00855at 177.712 kb on + strand, within BWI76_RS00855at 177.719 kb on - strand, within BWI76_RS00855at 177.744 kb on - strand, within BWI76_RS00855at 178.145 kb on - strandat 178.161 kb on - strandat 178.161 kb on - strandat 178.175 kb on + strandat 178.551 kb on - strand, within BWI76_RS00865at 178.618 kb on - strand, within BWI76_RS00865at 178.761 kb on - strand, within BWI76_RS00865at 178.943 kb on + strand, within BWI76_RS00865at 179.153 kb on + strand, within BWI76_RS00865at 179.208 kb on - strand, within BWI76_RS00865at 179.279 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
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177,458 + BWI76_RS00855 0.49 +1.7
177,466 - BWI76_RS00855 0.49 -0.0
177,598 + BWI76_RS00855 0.59 +0.1
177,712 + BWI76_RS00855 0.67 +0.6
177,719 - BWI76_RS00855 0.68 +0.4
177,744 - BWI76_RS00855 0.70 -0.3
178,145 - -0.2
178,161 - -1.0
178,161 - -0.2
178,175 + +0.3
178,551 - BWI76_RS00865 0.13 +1.2
178,618 - BWI76_RS00865 0.20 +1.0
178,761 - BWI76_RS00865 0.36 +1.1
178,943 + BWI76_RS00865 0.56 -0.8
179,153 + BWI76_RS00865 0.79 +0.4
179,208 - BWI76_RS00865 0.85 +0.7
179,279 - +2.0

Or see this region's nucleotide sequence