Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20090

Experiment: Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.2X

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20080 and BWI76_RS20085 are separated by 67 nucleotidesBWI76_RS20085 and BWI76_RS20090 are separated by 60 nucleotidesBWI76_RS20090 and BWI76_RS20095 are separated by 48 nucleotidesBWI76_RS20095 and BWI76_RS20100 are separated by 34 nucleotides BWI76_RS20080: BWI76_RS20080 - isochorismate synthase MenF, at 4,206,944 to 4,208,269 _RS20080 BWI76_RS20085: BWI76_RS20085 - GNAT family N-acetyltransferase, at 4,208,337 to 4,208,798 _RS20085 BWI76_RS20090: BWI76_RS20090 - ribonuclease Z, at 4,208,859 to 4,209,776 _RS20090 BWI76_RS20095: BWI76_RS20095 - tyrosine recombinase, at 4,209,825 to 4,210,355 _RS20095 BWI76_RS20100: BWI76_RS20100 - fimbrial protein, at 4,210,390 to 4,210,899 _RS20100 Position (kb) 4208 4209 4210Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4207.872 kb on - strand, within BWI76_RS20080at 4207.911 kb on - strand, within BWI76_RS20080at 4207.911 kb on - strand, within BWI76_RS20080at 4208.076 kb on + strand, within BWI76_RS20080at 4208.122 kb on + strand, within BWI76_RS20080at 4208.203 kb on + strandat 4208.408 kb on + strand, within BWI76_RS20085at 4208.525 kb on - strand, within BWI76_RS20085at 4208.743 kb on + strand, within BWI76_RS20085at 4208.799 kb on - strandat 4208.923 kb on - strandat 4209.076 kb on + strand, within BWI76_RS20090at 4209.110 kb on - strand, within BWI76_RS20090at 4209.144 kb on - strand, within BWI76_RS20090at 4209.223 kb on + strand, within BWI76_RS20090at 4209.396 kb on - strand, within BWI76_RS20090at 4209.564 kb on - strand, within BWI76_RS20090at 4209.682 kb on + strand, within BWI76_RS20090at 4209.758 kb on + strandat 4209.758 kb on + strandat 4209.758 kb on + strandat 4209.766 kb on - strandat 4209.766 kb on - strandat 4209.807 kb on - strandat 4209.830 kb on + strandat 4209.836 kb on - strandat 4209.838 kb on - strandat 4209.838 kb on - strandat 4209.843 kb on - strandat 4209.854 kb on + strandat 4209.880 kb on + strand, within BWI76_RS20095at 4209.885 kb on + strandat 4209.885 kb on + strand, within BWI76_RS20095at 4209.885 kb on + strand, within BWI76_RS20095at 4209.885 kb on + strand, within BWI76_RS20095at 4209.885 kb on + strand, within BWI76_RS20095at 4209.885 kb on + strand, within BWI76_RS20095at 4209.885 kb on + strand, within BWI76_RS20095at 4209.885 kb on + strand, within BWI76_RS20095at 4209.885 kb on + strandat 4209.885 kb on + strand, within BWI76_RS20095at 4209.890 kb on + strand, within BWI76_RS20095at 4209.893 kb on - strand, within BWI76_RS20095at 4209.893 kb on - strand, within BWI76_RS20095at 4209.893 kb on - strand, within BWI76_RS20095at 4209.893 kb on - strand, within BWI76_RS20095at 4209.893 kb on - strand, within BWI76_RS20095at 4209.893 kb on - strand, within BWI76_RS20095at 4209.893 kb on - strand, within BWI76_RS20095at 4209.893 kb on - strand, within BWI76_RS20095at 4209.893 kb on - strand, within BWI76_RS20095at 4209.897 kb on + strand, within BWI76_RS20095at 4209.923 kb on + strand, within BWI76_RS20095at 4209.923 kb on + strand, within BWI76_RS20095at 4209.923 kb on + strand, within BWI76_RS20095at 4209.923 kb on + strand, within BWI76_RS20095at 4209.923 kb on + strand, within BWI76_RS20095at 4209.923 kb on + strandat 4209.923 kb on + strand, within BWI76_RS20095at 4209.923 kb on + strand, within BWI76_RS20095at 4209.923 kb on + strand, within BWI76_RS20095at 4209.923 kb on + strand, within BWI76_RS20095at 4209.923 kb on + strand, within BWI76_RS20095at 4209.923 kb on + strand, within BWI76_RS20095at 4209.923 kb on + strand, within BWI76_RS20095at 4209.923 kb on + strand, within BWI76_RS20095at 4209.931 kb on - strand, within BWI76_RS20095at 4209.931 kb on - strand, within BWI76_RS20095at 4209.931 kb on - strand, within BWI76_RS20095at 4209.931 kb on - strand, within BWI76_RS20095at 4209.931 kb on - strand, within BWI76_RS20095at 4209.931 kb on - strand, within BWI76_RS20095at 4209.931 kb on - strand, within BWI76_RS20095at 4209.931 kb on - strand, within BWI76_RS20095at 4209.931 kb on - strand, within BWI76_RS20095at 4209.931 kb on - strand, within BWI76_RS20095at 4209.931 kb on - strand, within BWI76_RS20095at 4209.931 kb on - strand, within BWI76_RS20095at 4209.931 kb on - strand, within BWI76_RS20095at 4209.931 kb on - strand, within BWI76_RS20095at 4209.931 kb on - strandat 4209.931 kb on - strand, within BWI76_RS20095at 4209.931 kb on - strand, within BWI76_RS20095at 4209.931 kb on - strand, within BWI76_RS20095at 4209.931 kb on - strand, within BWI76_RS20095at 4209.931 kb on - strand, within BWI76_RS20095at 4209.931 kb on - strand, within BWI76_RS20095at 4209.938 kb on - strand, within BWI76_RS20095at 4209.944 kb on - strand, within BWI76_RS20095at 4209.955 kb on - strand, within BWI76_RS20095at 4209.956 kb on + strand, within BWI76_RS20095at 4209.964 kb on - strand, within BWI76_RS20095at 4210.072 kb on + strand, within BWI76_RS20095at 4210.072 kb on + strand, within BWI76_RS20095at 4210.072 kb on + strand, within BWI76_RS20095at 4210.072 kb on + strand, within BWI76_RS20095at 4210.072 kb on + strand, within BWI76_RS20095at 4210.072 kb on + strand, within BWI76_RS20095at 4210.072 kb on + strandat 4210.080 kb on - strand, within BWI76_RS20095at 4210.080 kb on - strand, within BWI76_RS20095at 4210.080 kb on - strand, within BWI76_RS20095at 4210.080 kb on - strand, within BWI76_RS20095at 4210.080 kb on - strand, within BWI76_RS20095at 4210.080 kb on - strand, within BWI76_RS20095at 4210.080 kb on - strand, within BWI76_RS20095at 4210.080 kb on - strand, within BWI76_RS20095at 4210.085 kb on - strand, within BWI76_RS20095at 4210.096 kb on - strand, within BWI76_RS20095at 4210.098 kb on + strand, within BWI76_RS20095at 4210.098 kb on + strand, within BWI76_RS20095at 4210.098 kb on + strand, within BWI76_RS20095at 4210.098 kb on + strand, within BWI76_RS20095at 4210.098 kb on + strand, within BWI76_RS20095at 4210.106 kb on - strand, within BWI76_RS20095at 4210.111 kb on - strand, within BWI76_RS20095at 4210.111 kb on - strandat 4210.111 kb on - strand, within BWI76_RS20095at 4210.115 kb on + strand, within BWI76_RS20095at 4210.132 kb on - strand, within BWI76_RS20095at 4210.237 kb on - strand, within BWI76_RS20095at 4210.237 kb on - strandat 4210.259 kb on - strand, within BWI76_RS20095at 4210.259 kb on - strand, within BWI76_RS20095at 4210.285 kb on + strand, within BWI76_RS20095at 4210.285 kb on + strand, within BWI76_RS20095at 4210.285 kb on + strand, within BWI76_RS20095at 4210.285 kb on + strand, within BWI76_RS20095at 4210.291 kb on - strand, within BWI76_RS20095at 4210.293 kb on - strand, within BWI76_RS20095at 4210.293 kb on - strand, within BWI76_RS20095at 4210.293 kb on - strand, within BWI76_RS20095at 4210.293 kb on - strand, within BWI76_RS20095at 4210.293 kb on - strand, within BWI76_RS20095at 4210.311 kb on - strandat 4210.312 kb on - strandat 4210.322 kb on - strandat 4210.324 kb on - strandat 4210.381 kb on + strandat 4210.390 kb on - strandat 4210.391 kb on - strandat 4210.396 kb on - strandat 4210.399 kb on + strandat 4210.407 kb on - strandat 4210.412 kb on - strandat 4210.429 kb on - strandat 4210.437 kb on + strandat 4210.437 kb on + strandat 4210.442 kb on + strand, within BWI76_RS20100at 4210.442 kb on + strand, within BWI76_RS20100at 4210.447 kb on + strand, within BWI76_RS20100at 4210.447 kb on + strand, within BWI76_RS20100at 4210.450 kb on - strand, within BWI76_RS20100at 4210.450 kb on - strand, within BWI76_RS20100at 4210.450 kb on - strand, within BWI76_RS20100at 4210.455 kb on - strand, within BWI76_RS20100at 4210.460 kb on - strand, within BWI76_RS20100at 4210.501 kb on + strand, within BWI76_RS20100at 4210.501 kb on + strand, within BWI76_RS20100at 4210.509 kb on - strand, within BWI76_RS20100at 4210.509 kb on - strand, within BWI76_RS20100at 4210.522 kb on - strand, within BWI76_RS20100at 4210.541 kb on + strand, within BWI76_RS20100at 4210.564 kb on - strand, within BWI76_RS20100at 4210.579 kb on + strand, within BWI76_RS20100at 4210.579 kb on + strand, within BWI76_RS20100at 4210.580 kb on - strand, within BWI76_RS20100at 4210.580 kb on - strand, within BWI76_RS20100at 4210.580 kb on - strand, within BWI76_RS20100at 4210.582 kb on - strand, within BWI76_RS20100at 4210.582 kb on - strand, within BWI76_RS20100at 4210.582 kb on - strand, within BWI76_RS20100at 4210.587 kb on + strand, within BWI76_RS20100at 4210.587 kb on + strand, within BWI76_RS20100at 4210.587 kb on - strand, within BWI76_RS20100at 4210.587 kb on - strand, within BWI76_RS20100at 4210.587 kb on - strand, within BWI76_RS20100at 4210.595 kb on - strand, within BWI76_RS20100at 4210.599 kb on + strand, within BWI76_RS20100at 4210.606 kb on - strand, within BWI76_RS20100at 4210.609 kb on + strand, within BWI76_RS20100at 4210.617 kb on - strand, within BWI76_RS20100at 4210.635 kb on + strand, within BWI76_RS20100at 4210.643 kb on + strandat 4210.655 kb on + strandat 4210.656 kb on - strand, within BWI76_RS20100at 4210.669 kb on + strand, within BWI76_RS20100at 4210.669 kb on + strand, within BWI76_RS20100at 4210.669 kb on + strand, within BWI76_RS20100at 4210.670 kb on - strandat 4210.674 kb on - strand, within BWI76_RS20100at 4210.692 kb on - strand, within BWI76_RS20100at 4210.706 kb on - strand, within BWI76_RS20100at 4210.716 kb on + strand, within BWI76_RS20100at 4210.722 kb on - strand, within BWI76_RS20100

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Per-strain Table

Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.2X
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4,207,872 - BWI76_RS20080 0.70 -0.5
4,207,911 - BWI76_RS20080 0.73 +0.1
4,207,911 - BWI76_RS20080 0.73 -0.4
4,208,076 + BWI76_RS20080 0.85 +0.8
4,208,122 + BWI76_RS20080 0.89 +0.8
4,208,203 + +0.1
4,208,408 + BWI76_RS20085 0.15 +0.1
4,208,525 - BWI76_RS20085 0.41 -0.1
4,208,743 + BWI76_RS20085 0.88 +0.5
4,208,799 - +0.8
4,208,923 - -2.8
4,209,076 + BWI76_RS20090 0.24 -1.7
4,209,110 - BWI76_RS20090 0.27 -0.5
4,209,144 - BWI76_RS20090 0.31 +0.1
4,209,223 + BWI76_RS20090 0.40 -0.9
4,209,396 - BWI76_RS20090 0.58 -0.4
4,209,564 - BWI76_RS20090 0.77 -0.8
4,209,682 + BWI76_RS20090 0.90 -1.9
4,209,758 + +0.9
4,209,758 + -0.4
4,209,758 + +0.1
4,209,766 - +0.2
4,209,766 - +0.1
4,209,807 - +0.2
4,209,830 + +0.5
4,209,836 - -0.1
4,209,838 - +0.2
4,209,838 - -0.4
4,209,843 - -0.2
4,209,854 + -0.4
4,209,880 + BWI76_RS20095 0.10 +0.4
4,209,885 + -0.5
4,209,885 + BWI76_RS20095 0.11 -0.5
4,209,885 + BWI76_RS20095 0.11 -0.4
4,209,885 + BWI76_RS20095 0.11 -0.1
4,209,885 + BWI76_RS20095 0.11 +0.1
4,209,885 + BWI76_RS20095 0.11 +0.9
4,209,885 + BWI76_RS20095 0.11 -0.3
4,209,885 + BWI76_RS20095 0.11 -0.4
4,209,885 + +0.6
4,209,885 + BWI76_RS20095 0.11 -0.0
4,209,890 + BWI76_RS20095 0.12 +0.4
4,209,893 - BWI76_RS20095 0.13 +0.1
4,209,893 - BWI76_RS20095 0.13 +0.9
4,209,893 - BWI76_RS20095 0.13 -0.2
4,209,893 - BWI76_RS20095 0.13 +0.8
4,209,893 - BWI76_RS20095 0.13 -0.1
4,209,893 - BWI76_RS20095 0.13 -0.1
4,209,893 - BWI76_RS20095 0.13 +0.1
4,209,893 - BWI76_RS20095 0.13 -0.1
4,209,893 - BWI76_RS20095 0.13 +0.2
4,209,897 + BWI76_RS20095 0.14 -0.1
4,209,923 + BWI76_RS20095 0.18 +0.1
4,209,923 + BWI76_RS20095 0.18 +0.9
4,209,923 + BWI76_RS20095 0.18 -0.2
4,209,923 + BWI76_RS20095 0.18 +0.2
4,209,923 + BWI76_RS20095 0.18 -0.2
4,209,923 + +1.4
4,209,923 + BWI76_RS20095 0.18 +0.6
4,209,923 + BWI76_RS20095 0.18 +0.3
4,209,923 + BWI76_RS20095 0.18 +0.9
4,209,923 + BWI76_RS20095 0.18 -0.5
4,209,923 + BWI76_RS20095 0.18 -0.4
4,209,923 + BWI76_RS20095 0.18 +0.4
4,209,923 + BWI76_RS20095 0.18 -0.1
4,209,923 + BWI76_RS20095 0.18 -0.1
4,209,931 - BWI76_RS20095 0.20 -0.4
4,209,931 - BWI76_RS20095 0.20 +2.2
4,209,931 - BWI76_RS20095 0.20 +1.2
4,209,931 - BWI76_RS20095 0.20 +0.4
4,209,931 - BWI76_RS20095 0.20 +0.3
4,209,931 - BWI76_RS20095 0.20 +0.1
4,209,931 - BWI76_RS20095 0.20 -0.1
4,209,931 - BWI76_RS20095 0.20 +0.2
4,209,931 - BWI76_RS20095 0.20 +0.1
4,209,931 - BWI76_RS20095 0.20 +0.5
4,209,931 - BWI76_RS20095 0.20 -0.1
4,209,931 - BWI76_RS20095 0.20 -0.0
4,209,931 - BWI76_RS20095 0.20 -0.4
4,209,931 - BWI76_RS20095 0.20 -0.5
4,209,931 - +2.0
4,209,931 - BWI76_RS20095 0.20 +0.8
4,209,931 - BWI76_RS20095 0.20 -0.1
4,209,931 - BWI76_RS20095 0.20 -0.0
4,209,931 - BWI76_RS20095 0.20 -0.1
4,209,931 - BWI76_RS20095 0.20 +0.2
4,209,931 - BWI76_RS20095 0.20 -0.6
4,209,938 - BWI76_RS20095 0.21 +0.3
4,209,944 - BWI76_RS20095 0.22 -0.2
4,209,955 - BWI76_RS20095 0.24 -0.1
4,209,956 + BWI76_RS20095 0.25 -0.1
4,209,964 - BWI76_RS20095 0.26 +0.9
4,210,072 + BWI76_RS20095 0.47 +1.4
4,210,072 + BWI76_RS20095 0.47 +0.0
4,210,072 + BWI76_RS20095 0.47 -0.1
4,210,072 + BWI76_RS20095 0.47 -0.8
4,210,072 + BWI76_RS20095 0.47 +0.4
4,210,072 + BWI76_RS20095 0.47 -0.6
4,210,072 + -1.3
4,210,080 - BWI76_RS20095 0.48 +0.0
4,210,080 - BWI76_RS20095 0.48 +1.2
4,210,080 - BWI76_RS20095 0.48 +0.2
4,210,080 - BWI76_RS20095 0.48 -0.3
4,210,080 - BWI76_RS20095 0.48 +0.2
4,210,080 - BWI76_RS20095 0.48 +0.0
4,210,080 - BWI76_RS20095 0.48 +0.5
4,210,080 - BWI76_RS20095 0.48 -0.7
4,210,085 - BWI76_RS20095 0.49 -0.6
4,210,096 - BWI76_RS20095 0.51 -0.1
4,210,098 + BWI76_RS20095 0.51 -0.6
4,210,098 + BWI76_RS20095 0.51 +0.3
4,210,098 + BWI76_RS20095 0.51 +0.6
4,210,098 + BWI76_RS20095 0.51 -1.1
4,210,098 + BWI76_RS20095 0.51 +0.7
4,210,106 - BWI76_RS20095 0.53 +1.8
4,210,111 - BWI76_RS20095 0.54 +1.2
4,210,111 - +0.2
4,210,111 - BWI76_RS20095 0.54 +0.5
4,210,115 + BWI76_RS20095 0.55 -1.2
4,210,132 - BWI76_RS20095 0.58 +0.1
4,210,237 - BWI76_RS20095 0.78 +1.2
4,210,237 - -0.4
4,210,259 - BWI76_RS20095 0.82 -0.1
4,210,259 - BWI76_RS20095 0.82 -0.0
4,210,285 + BWI76_RS20095 0.87 +0.2
4,210,285 + BWI76_RS20095 0.87 -1.2
4,210,285 + BWI76_RS20095 0.87 -0.3
4,210,285 + BWI76_RS20095 0.87 +0.6
4,210,291 - BWI76_RS20095 0.88 +0.3
4,210,293 - BWI76_RS20095 0.88 -0.5
4,210,293 - BWI76_RS20095 0.88 -0.7
4,210,293 - BWI76_RS20095 0.88 +0.8
4,210,293 - BWI76_RS20095 0.88 -0.2
4,210,293 - BWI76_RS20095 0.88 -0.4
4,210,311 - -0.9
4,210,312 - +0.5
4,210,322 - +0.6
4,210,324 - +0.7
4,210,381 + +0.4
4,210,390 - +0.6
4,210,391 - -1.2
4,210,396 - -0.0
4,210,399 + -0.1
4,210,407 - +0.1
4,210,412 - -0.7
4,210,429 - +0.5
4,210,437 + +0.9
4,210,437 + -0.3
4,210,442 + BWI76_RS20100 0.10 -0.5
4,210,442 + BWI76_RS20100 0.10 -0.0
4,210,447 + BWI76_RS20100 0.11 +0.0
4,210,447 + BWI76_RS20100 0.11 +0.2
4,210,450 - BWI76_RS20100 0.12 +0.2
4,210,450 - BWI76_RS20100 0.12 +0.1
4,210,450 - BWI76_RS20100 0.12 -0.6
4,210,455 - BWI76_RS20100 0.13 +0.5
4,210,460 - BWI76_RS20100 0.14 +1.0
4,210,501 + BWI76_RS20100 0.22 -0.4
4,210,501 + BWI76_RS20100 0.22 -0.3
4,210,509 - BWI76_RS20100 0.23 -0.0
4,210,509 - BWI76_RS20100 0.23 +0.0
4,210,522 - BWI76_RS20100 0.26 +0.8
4,210,541 + BWI76_RS20100 0.30 +0.4
4,210,564 - BWI76_RS20100 0.34 -0.4
4,210,579 + BWI76_RS20100 0.37 -0.1
4,210,579 + BWI76_RS20100 0.37 +0.3
4,210,580 - BWI76_RS20100 0.37 +0.1
4,210,580 - BWI76_RS20100 0.37 +0.2
4,210,580 - BWI76_RS20100 0.37 +0.4
4,210,582 - BWI76_RS20100 0.38 +0.1
4,210,582 - BWI76_RS20100 0.38 +0.2
4,210,582 - BWI76_RS20100 0.38 +1.7
4,210,587 + BWI76_RS20100 0.39 -0.2
4,210,587 + BWI76_RS20100 0.39 +0.8
4,210,587 - BWI76_RS20100 0.39 -0.2
4,210,587 - BWI76_RS20100 0.39 +0.2
4,210,587 - BWI76_RS20100 0.39 -0.4
4,210,595 - BWI76_RS20100 0.40 -0.7
4,210,599 + BWI76_RS20100 0.41 +1.5
4,210,606 - BWI76_RS20100 0.42 -0.4
4,210,609 + BWI76_RS20100 0.43 -0.5
4,210,617 - BWI76_RS20100 0.45 -1.6
4,210,635 + BWI76_RS20100 0.48 +0.3
4,210,643 + +0.8
4,210,655 + +1.0
4,210,656 - BWI76_RS20100 0.52 -0.4
4,210,669 + BWI76_RS20100 0.55 -1.1
4,210,669 + BWI76_RS20100 0.55 +0.1
4,210,669 + BWI76_RS20100 0.55 +0.0
4,210,670 - -0.2
4,210,674 - BWI76_RS20100 0.56 -0.5
4,210,692 - BWI76_RS20100 0.59 +0.4
4,210,706 - BWI76_RS20100 0.62 -0.3
4,210,716 + BWI76_RS20100 0.64 +0.5
4,210,722 - BWI76_RS20100 0.65 -0.2

Or see this region's nucleotide sequence