Experiment: Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12340 and BWI76_RS12345 are separated by 123 nucleotides BWI76_RS12345 and BWI76_RS12350 overlap by 4 nucleotides BWI76_RS12350 and BWI76_RS12355 are separated by 79 nucleotides
BWI76_RS12340: BWI76_RS12340 - molecular chaperone Tir, at 2,575,395 to 2,576,276
_RS12340
BWI76_RS12345: BWI76_RS12345 - nitrite transporter, at 2,576,400 to 2,576,774
_RS12345
BWI76_RS12350: BWI76_RS12350 - kinase, at 2,576,771 to 2,579,849
_RS12350
BWI76_RS12355: BWI76_RS12355 - flagellar biosynthesis protein, at 2,579,929 to 2,580,297
_RS12355
Position (kb)
2576
2577
2578
2579
2580 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2575.860 kb on + strand, within BWI76_RS12340 at 2575.861 kb on - strand, within BWI76_RS12340 at 2576.142 kb on + strand, within BWI76_RS12340 at 2576.166 kb on - strand, within BWI76_RS12340 at 2576.168 kb on + strand, within BWI76_RS12340 at 2576.244 kb on + strand at 2576.244 kb on + strand at 2576.249 kb on + strand at 2576.259 kb on + strand at 2576.274 kb on + strand at 2576.274 kb on + strand at 2576.287 kb on + strand at 2576.300 kb on + strand at 2576.300 kb on + strand at 2576.301 kb on - strand at 2576.305 kb on + strand at 2576.305 kb on + strand at 2576.305 kb on + strand at 2576.305 kb on + strand at 2576.318 kb on - strand at 2576.322 kb on + strand at 2576.322 kb on + strand at 2576.359 kb on + strand at 2576.616 kb on - strand, within BWI76_RS12345 at 2576.656 kb on - strand, within BWI76_RS12345 at 2576.826 kb on + strand at 2576.867 kb on + strand at 2577.120 kb on + strand, within BWI76_RS12350 at 2577.120 kb on + strand, within BWI76_RS12350 at 2577.206 kb on + strand, within BWI76_RS12350 at 2577.206 kb on + strand, within BWI76_RS12350 at 2577.385 kb on - strand, within BWI76_RS12350 at 2577.429 kb on + strand, within BWI76_RS12350 at 2577.473 kb on + strand, within BWI76_RS12350 at 2577.792 kb on - strand, within BWI76_RS12350 at 2577.842 kb on + strand, within BWI76_RS12350 at 2577.850 kb on - strand, within BWI76_RS12350 at 2578.134 kb on - strand, within BWI76_RS12350 at 2578.180 kb on - strand, within BWI76_RS12350 at 2578.303 kb on + strand, within BWI76_RS12350 at 2578.381 kb on - strand, within BWI76_RS12350 at 2578.553 kb on + strand, within BWI76_RS12350 at 2578.610 kb on + strand, within BWI76_RS12350 at 2578.621 kb on - strand, within BWI76_RS12350 at 2578.897 kb on - strand, within BWI76_RS12350 at 2579.113 kb on + strand, within BWI76_RS12350 at 2579.941 kb on - strand at 2579.944 kb on + strand at 2580.049 kb on - strand, within BWI76_RS12355 at 2580.120 kb on + strand, within BWI76_RS12355 at 2580.169 kb on - strand, within BWI76_RS12355 at 2580.181 kb on + strand, within BWI76_RS12355 at 2580.183 kb on - strand, within BWI76_RS12355 at 2580.194 kb on - strand, within BWI76_RS12355 at 2580.199 kb on - strand, within BWI76_RS12355 at 2580.199 kb on - strand, within BWI76_RS12355 at 2580.199 kb on - strand, within BWI76_RS12355 at 2580.199 kb on - strand at 2580.201 kb on - strand, within BWI76_RS12355 at 2580.201 kb on - strand, within BWI76_RS12355 at 2580.212 kb on + strand, within BWI76_RS12355 at 2580.225 kb on + strand, within BWI76_RS12355 at 2580.261 kb on - strand at 2580.376 kb on - strand at 2580.457 kb on - strand at 2580.463 kb on - strand at 2580.465 kb on + strand at 2580.472 kb on + strand at 2580.473 kb on - strand at 2580.522 kb on + strand at 2580.532 kb on - strand at 2580.578 kb on - strand at 2580.610 kb on - strand at 2580.612 kb on + strand at 2580.615 kb on - strand at 2580.644 kb on - strand at 2580.729 kb on + strand at 2580.743 kb on + strand at 2580.801 kb on + strand at 2580.804 kb on - strand at 2580.816 kb on + strand at 2580.817 kb on + strand at 2580.827 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.2X remove 2,575,860 + BWI76_RS12340 0.53 +0.2 2,575,861 - BWI76_RS12340 0.53 -0.5 2,576,142 + BWI76_RS12340 0.85 -1.1 2,576,166 - BWI76_RS12340 0.87 -0.2 2,576,168 + BWI76_RS12340 0.88 -0.4 2,576,244 + -1.5 2,576,244 + -1.3 2,576,249 + -1.5 2,576,259 + -0.0 2,576,274 + -1.5 2,576,274 + -0.3 2,576,287 + +0.1 2,576,300 + +0.8 2,576,300 + -0.5 2,576,301 - -1.1 2,576,305 + +0.2 2,576,305 + -0.5 2,576,305 + -0.6 2,576,305 + -0.4 2,576,318 - +0.9 2,576,322 + -0.5 2,576,322 + -0.6 2,576,359 + -0.9 2,576,616 - BWI76_RS12345 0.58 -0.8 2,576,656 - BWI76_RS12345 0.68 -0.7 2,576,826 + -0.8 2,576,867 + -1.5 2,577,120 + BWI76_RS12350 0.11 -0.2 2,577,120 + BWI76_RS12350 0.11 -1.1 2,577,206 + BWI76_RS12350 0.14 -0.5 2,577,206 + BWI76_RS12350 0.14 -1.2 2,577,385 - BWI76_RS12350 0.20 -1.2 2,577,429 + BWI76_RS12350 0.21 -1.3 2,577,473 + BWI76_RS12350 0.23 -0.6 2,577,792 - BWI76_RS12350 0.33 -2.9 2,577,842 + BWI76_RS12350 0.35 -0.0 2,577,850 - BWI76_RS12350 0.35 -1.1 2,578,134 - BWI76_RS12350 0.44 -1.0 2,578,180 - BWI76_RS12350 0.46 -0.1 2,578,303 + BWI76_RS12350 0.50 -0.5 2,578,381 - BWI76_RS12350 0.52 -0.7 2,578,553 + BWI76_RS12350 0.58 -1.2 2,578,610 + BWI76_RS12350 0.60 -1.3 2,578,621 - BWI76_RS12350 0.60 +0.7 2,578,897 - BWI76_RS12350 0.69 -0.8 2,579,113 + BWI76_RS12350 0.76 -2.3 2,579,941 - -0.4 2,579,944 + -0.9 2,580,049 - BWI76_RS12355 0.33 -0.2 2,580,120 + BWI76_RS12355 0.52 -1.0 2,580,169 - BWI76_RS12355 0.65 -0.7 2,580,181 + BWI76_RS12355 0.68 -0.7 2,580,183 - BWI76_RS12355 0.69 -1.4 2,580,194 - BWI76_RS12355 0.72 -0.7 2,580,199 - BWI76_RS12355 0.73 -1.1 2,580,199 - BWI76_RS12355 0.73 -0.8 2,580,199 - BWI76_RS12355 0.73 -0.3 2,580,199 - -0.7 2,580,201 - BWI76_RS12355 0.74 -0.2 2,580,201 - BWI76_RS12355 0.74 +1.1 2,580,212 + BWI76_RS12355 0.77 -0.6 2,580,225 + BWI76_RS12355 0.80 +1.6 2,580,261 - -1.5 2,580,376 - -1.1 2,580,457 - -2.1 2,580,463 - -1.0 2,580,465 + -1.1 2,580,472 + +1.4 2,580,473 - -1.8 2,580,522 + -0.4 2,580,532 - -0.3 2,580,578 - -0.8 2,580,610 - -0.5 2,580,612 + -2.0 2,580,615 - -0.3 2,580,644 - +0.8 2,580,729 + -2.0 2,580,743 + -1.5 2,580,801 + -0.9 2,580,804 - -1.8 2,580,816 + -1.2 2,580,817 + +0.4 2,580,827 + -1.8
Or see this region's nucleotide sequence