Experiment: R2A control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23805 and BWI76_RS23810 are separated by 5 nucleotides BWI76_RS23810 and BWI76_RS23815 are separated by 22 nucleotides
BWI76_RS23805: BWI76_RS23805 - ssDNA exonuclease RecJ, at 4,969,297 to 4,971,030
_RS23805
BWI76_RS23810: BWI76_RS23810 - bifunctional protein-disulfide isomerase/oxidoreductase DsbC, at 4,971,036 to 4,971,749
_RS23810
BWI76_RS23815: BWI76_RS23815 - site-specific tyrosine recombinase XerD, at 4,971,772 to 4,972,668
_RS23815
Position (kb)
4971
4972 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4970.039 kb on - strand, within BWI76_RS23805 at 4970.088 kb on - strand, within BWI76_RS23805 at 4970.259 kb on - strand, within BWI76_RS23805 at 4970.285 kb on - strand, within BWI76_RS23805 at 4970.504 kb on - strand, within BWI76_RS23805 at 4970.575 kb on - strand, within BWI76_RS23805 at 4970.635 kb on - strand, within BWI76_RS23805 at 4970.820 kb on - strand, within BWI76_RS23805 at 4970.866 kb on + strand at 4970.879 kb on - strand at 4970.879 kb on - strand at 4971.158 kb on + strand, within BWI76_RS23810 at 4971.385 kb on - strand, within BWI76_RS23810 at 4971.469 kb on + strand at 4971.702 kb on + strand at 4971.711 kb on - strand at 4971.838 kb on - strand at 4971.856 kb on + strand at 4971.972 kb on + strand, within BWI76_RS23815 at 4972.185 kb on + strand, within BWI76_RS23815 at 4972.742 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control remove 4,970,039 - BWI76_RS23805 0.43 -0.3 4,970,088 - BWI76_RS23805 0.46 -1.8 4,970,259 - BWI76_RS23805 0.55 -0.2 4,970,285 - BWI76_RS23805 0.57 -0.6 4,970,504 - BWI76_RS23805 0.70 -1.7 4,970,575 - BWI76_RS23805 0.74 -0.3 4,970,635 - BWI76_RS23805 0.77 -0.5 4,970,820 - BWI76_RS23805 0.88 -0.7 4,970,866 + -0.4 4,970,879 - -0.5 4,970,879 - +0.1 4,971,158 + BWI76_RS23810 0.17 -0.5 4,971,385 - BWI76_RS23810 0.49 -1.0 4,971,469 + +1.5 4,971,702 + +0.2 4,971,711 - -0.1 4,971,838 - -2.2 4,971,856 + -3.3 4,971,972 + BWI76_RS23815 0.22 -1.5 4,972,185 + BWI76_RS23815 0.46 -1.8 4,972,742 + -0.3
Or see this region's nucleotide sequence