Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26615 and BWI76_RS26620 are separated by 331 nucleotides BWI76_RS26620 and BWI76_RS26625 are separated by 58 nucleotides BWI76_RS26625 and BWI76_RS26630 are separated by 37 nucleotides BWI76_RS26630 and BWI76_RS26635 are separated by 111 nucleotides
BWI76_RS26615: BWI76_RS26615 - gluconate:H+ symporter <small>(from data)</small>, at 5,510,053 to 5,511,369
_RS26615
BWI76_RS26620: BWI76_RS26620 - Fe-S biogenesis protein NfuA, at 5,511,701 to 5,512,276
_RS26620
BWI76_RS26625: BWI76_RS26625 - gluconate transporter periplasmic gluconate-binding protein, at 5,512,335 to 5,513,009
_RS26625
BWI76_RS26630: BWI76_RS26630 - pimeloyl-[acyl-carrier protein] methyl ester esterase, at 5,513,047 to 5,513,829
_RS26630
BWI76_RS26635: BWI76_RS26635 - hypothetical protein, at 5,513,941 to 5,514,210
_RS26635
Position (kb) 5512
5513
5514 Strain fitness (log2 ratio) -1
0
1
2 at 5511.370 kb on + strand at 5511.658 kb on - strand at 5511.659 kb on + strand at 5511.664 kb on - strand at 5512.114 kb on - strand, within BWI76_RS26620 at 5512.362 kb on - strand at 5512.511 kb on + strand, within BWI76_RS26625 at 5512.734 kb on + strand, within BWI76_RS26625 at 5512.743 kb on - strand, within BWI76_RS26625 at 5512.770 kb on - strand, within BWI76_RS26625 at 5512.860 kb on + strand, within BWI76_RS26625 at 5513.030 kb on + strand at 5513.108 kb on + strand at 5513.138 kb on + strand, within BWI76_RS26630 at 5513.141 kb on - strand, within BWI76_RS26630 at 5513.324 kb on - strand, within BWI76_RS26630 at 5513.375 kb on + strand, within BWI76_RS26630 at 5513.524 kb on + strand, within BWI76_RS26630 at 5513.669 kb on + strand, within BWI76_RS26630 at 5513.797 kb on + strand at 5513.930 kb on + strand at 5513.984 kb on + strand, within BWI76_RS26635 at 5513.988 kb on + strand, within BWI76_RS26635 at 5513.996 kb on - strand, within BWI76_RS26635
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 5,511,370 + -0.3 5,511,658 - +0.9 5,511,659 + +0.3 5,511,664 - -0.1 5,512,114 - BWI76_RS26620 0.72 -0.4 5,512,362 - +1.4 5,512,511 + BWI76_RS26625 0.26 +0.1 5,512,734 + BWI76_RS26625 0.59 -1.1 5,512,743 - BWI76_RS26625 0.60 -0.9 5,512,770 - BWI76_RS26625 0.64 -0.7 5,512,860 + BWI76_RS26625 0.78 -1.1 5,513,030 + +0.3 5,513,108 + -0.1 5,513,138 + BWI76_RS26630 0.12 -0.3 5,513,141 - BWI76_RS26630 0.12 -0.6 5,513,324 - BWI76_RS26630 0.35 +0.7 5,513,375 + BWI76_RS26630 0.42 +2.0 5,513,524 + BWI76_RS26630 0.61 +0.6 5,513,669 + BWI76_RS26630 0.79 -1.6 5,513,797 + +0.3 5,513,930 + -0.8 5,513,984 + BWI76_RS26635 0.16 +0.6 5,513,988 + BWI76_RS26635 0.17 -0.1 5,513,996 - BWI76_RS26635 0.20 +0.0
Or see this region's nucleotide sequence