Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20055 and BWI76_RS20060 overlap by 4 nucleotides BWI76_RS20060 and BWI76_RS20065 overlap by 1 nucleotides BWI76_RS20065 and BWI76_RS20070 are separated by 14 nucleotides BWI76_RS20070 and BWI76_RS20075 overlap by 4 nucleotides
BWI76_RS20055: BWI76_RS20055 - 2-succinylbenzoate-CoA ligase, at 4,201,216 to 4,202,607
_RS20055
BWI76_RS20060: BWI76_RS20060 - o-succinylbenzoate synthase, at 4,202,604 to 4,203,569
_RS20060
BWI76_RS20065: BWI76_RS20065 - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 4,203,569 to 4,204,426
_RS20065
BWI76_RS20070: BWI76_RS20070 - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase, at 4,204,441 to 4,205,199
_RS20070
BWI76_RS20075: BWI76_RS20075 - 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylic-acid synthase, at 4,205,196 to 4,206,866
_RS20075
Position (kb) 4203
4204
4205 Strain fitness (log2 ratio) -1
0
1
2 at 4202.602 kb on + strand at 4202.610 kb on - strand at 4202.632 kb on - strand at 4202.687 kb on + strand at 4202.728 kb on - strand, within BWI76_RS20060 at 4202.798 kb on + strand, within BWI76_RS20060 at 4202.935 kb on - strand, within BWI76_RS20060 at 4203.063 kb on - strand, within BWI76_RS20060 at 4203.151 kb on + strand, within BWI76_RS20060 at 4203.174 kb on - strand, within BWI76_RS20060 at 4203.306 kb on - strand, within BWI76_RS20060 at 4203.763 kb on - strand, within BWI76_RS20065 at 4203.827 kb on - strand, within BWI76_RS20065 at 4203.913 kb on + strand, within BWI76_RS20065 at 4204.105 kb on + strand, within BWI76_RS20065 at 4204.489 kb on + strand at 4204.777 kb on + strand, within BWI76_RS20070 at 4205.061 kb on + strand, within BWI76_RS20070 at 4205.182 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 4,202,602 + -0.3 4,202,610 - -0.1 4,202,632 - -1.0 4,202,687 + -0.2 4,202,728 - BWI76_RS20060 0.13 +0.3 4,202,798 + BWI76_RS20060 0.20 -0.4 4,202,935 - BWI76_RS20060 0.34 +0.1 4,203,063 - BWI76_RS20060 0.48 -0.3 4,203,151 + BWI76_RS20060 0.57 +1.9 4,203,174 - BWI76_RS20060 0.59 -0.2 4,203,306 - BWI76_RS20060 0.73 +0.0 4,203,763 - BWI76_RS20065 0.23 +0.2 4,203,827 - BWI76_RS20065 0.30 -1.0 4,203,913 + BWI76_RS20065 0.40 -1.0 4,204,105 + BWI76_RS20065 0.62 -1.4 4,204,489 + -0.0 4,204,777 + BWI76_RS20070 0.44 +2.3 4,205,061 + BWI76_RS20070 0.82 -1.4 4,205,182 - +0.6
Or see this region's nucleotide sequence