Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03915 and BWI76_RS03920 are separated by 70 nucleotides BWI76_RS03920 and BWI76_RS03925 are separated by 32 nucleotides BWI76_RS03925 and BWI76_RS03930 are separated by 3 nucleotides
BWI76_RS03915: BWI76_RS03915 - MurR/RpiR family transcriptional regulator, at 809,174 to 810,037
_RS03915
BWI76_RS03920: BWI76_RS03920 - N-acetyltransferase, at 810,108 to 810,641
_RS03920
BWI76_RS03925: BWI76_RS03925 - N-acetyltransferase GCN5, at 810,674 to 811,510
_RS03925
BWI76_RS03930: BWI76_RS03930 - MFS transporter, at 811,514 to 812,875
_RS03930
Position (kb)
810
811
812 Strain fitness (log2 ratio)
-1
0
1 at 809.718 kb on + strand, within BWI76_RS03915 at 809.768 kb on - strand, within BWI76_RS03915 at 809.998 kb on + strand at 810.120 kb on + strand at 810.158 kb on - strand at 810.197 kb on + strand, within BWI76_RS03920 at 810.349 kb on - strand, within BWI76_RS03920 at 810.421 kb on - strand, within BWI76_RS03920 at 810.649 kb on + strand at 810.669 kb on + strand at 810.687 kb on - strand at 810.723 kb on + strand at 810.810 kb on + strand, within BWI76_RS03925 at 811.013 kb on - strand, within BWI76_RS03925 at 811.067 kb on - strand, within BWI76_RS03925 at 811.141 kb on - strand, within BWI76_RS03925 at 811.142 kb on - strand, within BWI76_RS03925 at 811.274 kb on + strand, within BWI76_RS03925 at 811.557 kb on + strand at 811.654 kb on + strand, within BWI76_RS03930 at 811.693 kb on - strand, within BWI76_RS03930 at 811.693 kb on - strand, within BWI76_RS03930 at 811.695 kb on + strand, within BWI76_RS03930 at 811.714 kb on + strand, within BWI76_RS03930 at 811.770 kb on + strand, within BWI76_RS03930 at 811.775 kb on + strand, within BWI76_RS03930 at 811.775 kb on + strand, within BWI76_RS03930 at 811.845 kb on - strand at 811.851 kb on + strand, within BWI76_RS03930 at 811.851 kb on + strand, within BWI76_RS03930 at 812.017 kb on - strand, within BWI76_RS03930 at 812.054 kb on - strand at 812.059 kb on + strand, within BWI76_RS03930 at 812.212 kb on + strand, within BWI76_RS03930 at 812.351 kb on - strand, within BWI76_RS03930 at 812.358 kb on + strand, within BWI76_RS03930 at 812.366 kb on - strand, within BWI76_RS03930 at 812.366 kb on - strand, within BWI76_RS03930 at 812.432 kb on - strand, within BWI76_RS03930 at 812.509 kb on + strand, within BWI76_RS03930
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 809,718 + BWI76_RS03915 0.63 +0.6 809,768 - BWI76_RS03915 0.69 -0.2 809,998 + -0.1 810,120 + +0.5 810,158 - -0.0 810,197 + BWI76_RS03920 0.17 -1.0 810,349 - BWI76_RS03920 0.45 -0.5 810,421 - BWI76_RS03920 0.59 -0.3 810,649 + -1.1 810,669 + -1.0 810,687 - -0.5 810,723 + +0.1 810,810 + BWI76_RS03925 0.16 -0.0 811,013 - BWI76_RS03925 0.41 -1.7 811,067 - BWI76_RS03925 0.47 -0.2 811,141 - BWI76_RS03925 0.56 -1.1 811,142 - BWI76_RS03925 0.56 -0.5 811,274 + BWI76_RS03925 0.72 -1.5 811,557 + +1.2 811,654 + BWI76_RS03930 0.10 +0.4 811,693 - BWI76_RS03930 0.13 +0.1 811,693 - BWI76_RS03930 0.13 -0.4 811,695 + BWI76_RS03930 0.13 -0.2 811,714 + BWI76_RS03930 0.15 -0.0 811,770 + BWI76_RS03930 0.19 +0.8 811,775 + BWI76_RS03930 0.19 -0.7 811,775 + BWI76_RS03930 0.19 +0.3 811,845 - -1.5 811,851 + BWI76_RS03930 0.25 -1.2 811,851 + BWI76_RS03930 0.25 +0.6 812,017 - BWI76_RS03930 0.37 -0.1 812,054 - +0.1 812,059 + BWI76_RS03930 0.40 -0.8 812,212 + BWI76_RS03930 0.51 -0.6 812,351 - BWI76_RS03930 0.61 -1.0 812,358 + BWI76_RS03930 0.62 -0.4 812,366 - BWI76_RS03930 0.63 -0.9 812,366 - BWI76_RS03930 0.63 -0.1 812,432 - BWI76_RS03930 0.67 +0.1 812,509 + BWI76_RS03930 0.73 -0.8
Or see this region's nucleotide sequence