Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02045 and BWI76_RS02050 are separated by 103 nucleotides BWI76_RS02050 and BWI76_RS02055 overlap by 8 nucleotides BWI76_RS02055 and BWI76_RS02060 are separated by 607 nucleotides
BWI76_RS02045: BWI76_RS02045 - LysR family transcriptional regulator, at 421,398 to 422,294
_RS02045
BWI76_RS02050: BWI76_RS02050 - CidA/LrgA family protein, at 422,398 to 422,808
_RS02050
BWI76_RS02055: BWI76_RS02055 - murein hydrolase effector protein LrgB, at 422,801 to 423,490
_RS02055
BWI76_RS02060: BWI76_RS02060 - fimbrial chaperone protein, at 424,098 to 424,634
_RS02060
Position (kb) 422
423
424 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 421.950 kb on + strand, within BWI76_RS02045 at 422.000 kb on + strand, within BWI76_RS02045 at 422.148 kb on + strand, within BWI76_RS02045 at 422.161 kb on - strand, within BWI76_RS02045 at 422.308 kb on + strand at 422.316 kb on - strand at 422.316 kb on - strand at 422.355 kb on + strand at 422.355 kb on + strand at 422.355 kb on + strand at 422.355 kb on + strand at 422.355 kb on + strand at 422.355 kb on + strand at 422.355 kb on + strand at 422.356 kb on + strand at 422.356 kb on + strand at 422.363 kb on - strand at 422.363 kb on - strand at 422.363 kb on - strand at 422.363 kb on - strand at 422.363 kb on - strand at 422.363 kb on - strand at 422.363 kb on - strand at 422.363 kb on - strand at 422.368 kb on - strand at 422.415 kb on - strand at 422.418 kb on + strand at 422.429 kb on + strand at 422.429 kb on + strand at 422.437 kb on - strand at 422.461 kb on + strand, within BWI76_RS02050 at 422.496 kb on + strand, within BWI76_RS02050 at 422.782 kb on + strand at 422.792 kb on + strand at 422.792 kb on + strand at 422.792 kb on + strand at 422.816 kb on - strand at 422.978 kb on - strand, within BWI76_RS02055 at 423.081 kb on + strand, within BWI76_RS02055 at 423.182 kb on + strand, within BWI76_RS02055 at 423.271 kb on - strand, within BWI76_RS02055 at 423.339 kb on - strand, within BWI76_RS02055 at 423.352 kb on - strand, within BWI76_RS02055 at 423.384 kb on + strand, within BWI76_RS02055 at 423.384 kb on + strand, within BWI76_RS02055 at 423.392 kb on - strand, within BWI76_RS02055 at 423.396 kb on + strand, within BWI76_RS02055 at 423.467 kb on + strand at 423.467 kb on + strand at 423.474 kb on + strand at 423.474 kb on + strand at 423.484 kb on + strand at 423.484 kb on + strand at 423.484 kb on + strand at 423.484 kb on + strand at 423.484 kb on + strand at 423.484 kb on + strand at 423.487 kb on - strand at 423.492 kb on - strand at 423.492 kb on - strand at 423.492 kb on - strand at 423.492 kb on - strand at 423.492 kb on - strand at 423.492 kb on - strand at 423.492 kb on - strand at 423.492 kb on - strand at 423.492 kb on - strand at 423.492 kb on - strand at 423.492 kb on - strand at 423.492 kb on - strand at 423.493 kb on + strand at 423.500 kb on + strand at 423.503 kb on + strand at 423.511 kb on - strand at 423.522 kb on + strand at 423.525 kb on - strand at 423.525 kb on - strand at 423.525 kb on - strand at 423.525 kb on - strand at 423.530 kb on - strand at 423.559 kb on + strand at 423.559 kb on + strand at 423.559 kb on + strand at 423.559 kb on + strand at 423.559 kb on + strand at 423.559 kb on + strand at 423.559 kb on + strand at 423.567 kb on - strand at 423.567 kb on - strand at 423.567 kb on - strand at 423.567 kb on - strand at 423.621 kb on + strand at 423.861 kb on + strand at 423.861 kb on + strand at 423.878 kb on + strand at 423.899 kb on + strand at 424.006 kb on + strand at 424.076 kb on - strand at 424.082 kb on + strand at 424.122 kb on + strand at 424.128 kb on + strand at 424.133 kb on + strand at 424.148 kb on - strand at 424.150 kb on + strand at 424.157 kb on + strand, within BWI76_RS02060 at 424.158 kb on - strand, within BWI76_RS02060 at 424.158 kb on - strand, within BWI76_RS02060 at 424.165 kb on - strand, within BWI76_RS02060 at 424.176 kb on - strand, within BWI76_RS02060 at 424.181 kb on + strand, within BWI76_RS02060 at 424.181 kb on + strand, within BWI76_RS02060 at 424.183 kb on + strand, within BWI76_RS02060 at 424.183 kb on + strand, within BWI76_RS02060 at 424.183 kb on + strand, within BWI76_RS02060 at 424.183 kb on + strand at 424.183 kb on + strand, within BWI76_RS02060 at 424.183 kb on + strand, within BWI76_RS02060 at 424.183 kb on - strand, within BWI76_RS02060 at 424.191 kb on - strand at 424.191 kb on - strand, within BWI76_RS02060 at 424.191 kb on - strand, within BWI76_RS02060 at 424.191 kb on - strand, within BWI76_RS02060 at 424.191 kb on - strand, within BWI76_RS02060 at 424.191 kb on - strand, within BWI76_RS02060 at 424.191 kb on - strand, within BWI76_RS02060 at 424.191 kb on - strand, within BWI76_RS02060 at 424.209 kb on + strand, within BWI76_RS02060 at 424.209 kb on - strand, within BWI76_RS02060 at 424.214 kb on + strand, within BWI76_RS02060 at 424.214 kb on + strand, within BWI76_RS02060 at 424.214 kb on + strand, within BWI76_RS02060 at 424.214 kb on + strand, within BWI76_RS02060 at 424.214 kb on + strand, within BWI76_RS02060 at 424.214 kb on + strand, within BWI76_RS02060 at 424.214 kb on + strand, within BWI76_RS02060 at 424.214 kb on + strand, within BWI76_RS02060 at 424.214 kb on + strand, within BWI76_RS02060 at 424.214 kb on + strand, within BWI76_RS02060 at 424.214 kb on + strand, within BWI76_RS02060 at 424.214 kb on + strand, within BWI76_RS02060 at 424.219 kb on + strand, within BWI76_RS02060 at 424.222 kb on - strand, within BWI76_RS02060 at 424.222 kb on - strand, within BWI76_RS02060 at 424.222 kb on - strand, within BWI76_RS02060 at 424.222 kb on - strand, within BWI76_RS02060 at 424.222 kb on - strand, within BWI76_RS02060 at 424.222 kb on - strand, within BWI76_RS02060 at 424.240 kb on + strand, within BWI76_RS02060 at 424.240 kb on + strand, within BWI76_RS02060 at 424.259 kb on - strand, within BWI76_RS02060 at 424.292 kb on + strand, within BWI76_RS02060 at 424.295 kb on + strand, within BWI76_RS02060 at 424.300 kb on - strand, within BWI76_RS02060 at 424.301 kb on - strand, within BWI76_RS02060 at 424.303 kb on - strand, within BWI76_RS02060 at 424.313 kb on - strand, within BWI76_RS02060 at 424.318 kb on + strand, within BWI76_RS02060 at 424.318 kb on + strand, within BWI76_RS02060 at 424.323 kb on + strand, within BWI76_RS02060 at 424.336 kb on + strand, within BWI76_RS02060 at 424.336 kb on + strand, within BWI76_RS02060 at 424.336 kb on + strand, within BWI76_RS02060 at 424.336 kb on + strand, within BWI76_RS02060 at 424.336 kb on + strand, within BWI76_RS02060 at 424.336 kb on + strand, within BWI76_RS02060 at 424.336 kb on + strand, within BWI76_RS02060 at 424.336 kb on + strand, within BWI76_RS02060 at 424.336 kb on + strand, within BWI76_RS02060 at 424.336 kb on + strand, within BWI76_RS02060 at 424.337 kb on + strand, within BWI76_RS02060 at 424.337 kb on + strand, within BWI76_RS02060 at 424.344 kb on - strand, within BWI76_RS02060 at 424.345 kb on - strand, within BWI76_RS02060 at 424.348 kb on + strand, within BWI76_RS02060 at 424.348 kb on + strand, within BWI76_RS02060 at 424.348 kb on + strand, within BWI76_RS02060 at 424.348 kb on + strand, within BWI76_RS02060 at 424.348 kb on + strand, within BWI76_RS02060 at 424.348 kb on + strand, within BWI76_RS02060 at 424.348 kb on + strand, within BWI76_RS02060 at 424.348 kb on + strand, within BWI76_RS02060 at 424.348 kb on + strand, within BWI76_RS02060 at 424.348 kb on + strand, within BWI76_RS02060 at 424.348 kb on + strand, within BWI76_RS02060 at 424.348 kb on + strand, within BWI76_RS02060 at 424.348 kb on + strand, within BWI76_RS02060 at 424.348 kb on + strand, within BWI76_RS02060 at 424.348 kb on + strand, within BWI76_RS02060 at 424.349 kb on - strand, within BWI76_RS02060 at 424.356 kb on - strand, within BWI76_RS02060 at 424.356 kb on - strand, within BWI76_RS02060 at 424.356 kb on - strand, within BWI76_RS02060 at 424.358 kb on + strand, within BWI76_RS02060 at 424.358 kb on + strand, within BWI76_RS02060 at 424.358 kb on + strand, within BWI76_RS02060 at 424.361 kb on - strand, within BWI76_RS02060 at 424.425 kb on + strand, within BWI76_RS02060 at 424.425 kb on + strand, within BWI76_RS02060 at 424.425 kb on + strand, within BWI76_RS02060 at 424.433 kb on + strand, within BWI76_RS02060 at 424.433 kb on + strand, within BWI76_RS02060 at 424.433 kb on + strand, within BWI76_RS02060 at 424.433 kb on + strand, within BWI76_RS02060 at 424.433 kb on + strand, within BWI76_RS02060 at 424.441 kb on - strand, within BWI76_RS02060
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 421,950 + BWI76_RS02045 0.62 +0.9 422,000 + BWI76_RS02045 0.67 -1.0 422,148 + BWI76_RS02045 0.84 +1.1 422,161 - BWI76_RS02045 0.85 -0.0 422,308 + -0.1 422,316 - +0.0 422,316 - -1.1 422,355 + +0.3 422,355 + +0.3 422,355 + +0.2 422,355 + +0.3 422,355 + -1.3 422,355 + +0.2 422,355 + +0.8 422,356 + -1.0 422,356 + -0.5 422,363 - +0.7 422,363 - -0.1 422,363 - -0.6 422,363 - +0.3 422,363 - +0.0 422,363 - +0.0 422,363 - +0.1 422,363 - -1.0 422,368 - +0.6 422,415 - +0.6 422,418 + +1.6 422,429 + -0.0 422,429 + -0.1 422,437 - -2.9 422,461 + BWI76_RS02050 0.15 +0.2 422,496 + BWI76_RS02050 0.24 +0.8 422,782 + -0.3 422,792 + +2.0 422,792 + -0.6 422,792 + +0.9 422,816 - -0.6 422,978 - BWI76_RS02055 0.26 +0.3 423,081 + BWI76_RS02055 0.41 +0.5 423,182 + BWI76_RS02055 0.55 +2.3 423,271 - BWI76_RS02055 0.68 -0.2 423,339 - BWI76_RS02055 0.78 -0.3 423,352 - BWI76_RS02055 0.80 +0.0 423,384 + BWI76_RS02055 0.84 -0.1 423,384 + BWI76_RS02055 0.84 +1.0 423,392 - BWI76_RS02055 0.86 +0.1 423,396 + BWI76_RS02055 0.86 +0.4 423,467 + -0.3 423,467 + -0.0 423,474 + +0.1 423,474 + +0.7 423,484 + +0.8 423,484 + -0.2 423,484 + +0.8 423,484 + -1.2 423,484 + -0.4 423,484 + -0.2 423,487 - +0.3 423,492 - +1.7 423,492 - -0.2 423,492 - -0.6 423,492 - +0.1 423,492 - -0.9 423,492 - -0.6 423,492 - -0.1 423,492 - -0.1 423,492 - -0.4 423,492 - +0.8 423,492 - -0.3 423,492 - +2.9 423,493 + -2.6 423,500 + -0.1 423,503 + +0.1 423,511 - -1.6 423,522 + -0.9 423,525 - +0.7 423,525 - +0.4 423,525 - +0.1 423,525 - -0.3 423,530 - +0.1 423,559 + +0.3 423,559 + -0.2 423,559 + -0.1 423,559 + +0.4 423,559 + +0.9 423,559 + +0.4 423,559 + -0.7 423,567 - -0.6 423,567 - +0.2 423,567 - -1.4 423,567 - -0.3 423,621 + +1.0 423,861 + +0.6 423,861 + +0.6 423,878 + -0.1 423,899 + +0.6 424,006 + -0.2 424,076 - +1.5 424,082 + -0.7 424,122 + +1.2 424,128 + -0.2 424,133 + +5.6 424,148 - -0.1 424,150 + +0.6 424,157 + BWI76_RS02060 0.11 +0.5 424,158 - BWI76_RS02060 0.11 +0.3 424,158 - BWI76_RS02060 0.11 +0.1 424,165 - BWI76_RS02060 0.12 -0.5 424,176 - BWI76_RS02060 0.15 +0.5 424,181 + BWI76_RS02060 0.15 +1.1 424,181 + BWI76_RS02060 0.15 +1.0 424,183 + BWI76_RS02060 0.16 +1.0 424,183 + BWI76_RS02060 0.16 +0.2 424,183 + BWI76_RS02060 0.16 -0.2 424,183 + +1.5 424,183 + BWI76_RS02060 0.16 -0.1 424,183 + BWI76_RS02060 0.16 +0.1 424,183 - BWI76_RS02060 0.16 -1.5 424,191 - -0.7 424,191 - BWI76_RS02060 0.17 -0.2 424,191 - BWI76_RS02060 0.17 +1.3 424,191 - BWI76_RS02060 0.17 +0.5 424,191 - BWI76_RS02060 0.17 +0.1 424,191 - BWI76_RS02060 0.17 -0.5 424,191 - BWI76_RS02060 0.17 +0.5 424,191 - BWI76_RS02060 0.17 -0.9 424,209 + BWI76_RS02060 0.21 -1.7 424,209 - BWI76_RS02060 0.21 -0.3 424,214 + BWI76_RS02060 0.22 -0.2 424,214 + BWI76_RS02060 0.22 -0.2 424,214 + BWI76_RS02060 0.22 -0.4 424,214 + BWI76_RS02060 0.22 -0.0 424,214 + BWI76_RS02060 0.22 +0.5 424,214 + BWI76_RS02060 0.22 -0.5 424,214 + BWI76_RS02060 0.22 -0.3 424,214 + BWI76_RS02060 0.22 -0.5 424,214 + BWI76_RS02060 0.22 +0.1 424,214 + BWI76_RS02060 0.22 +0.5 424,214 + BWI76_RS02060 0.22 +0.4 424,214 + BWI76_RS02060 0.22 +1.1 424,219 + BWI76_RS02060 0.23 +1.0 424,222 - BWI76_RS02060 0.23 +0.2 424,222 - BWI76_RS02060 0.23 +0.6 424,222 - BWI76_RS02060 0.23 -0.6 424,222 - BWI76_RS02060 0.23 +0.0 424,222 - BWI76_RS02060 0.23 +0.1 424,222 - BWI76_RS02060 0.23 -0.7 424,240 + BWI76_RS02060 0.26 +0.9 424,240 + BWI76_RS02060 0.26 +0.5 424,259 - BWI76_RS02060 0.30 +0.3 424,292 + BWI76_RS02060 0.36 -0.2 424,295 + BWI76_RS02060 0.37 -0.8 424,300 - BWI76_RS02060 0.38 -0.5 424,301 - BWI76_RS02060 0.38 -0.1 424,303 - BWI76_RS02060 0.38 -0.2 424,313 - BWI76_RS02060 0.40 -0.3 424,318 + BWI76_RS02060 0.41 +0.5 424,318 + BWI76_RS02060 0.41 +0.5 424,323 + BWI76_RS02060 0.42 +0.4 424,336 + BWI76_RS02060 0.44 +0.2 424,336 + BWI76_RS02060 0.44 -0.1 424,336 + BWI76_RS02060 0.44 -0.2 424,336 + BWI76_RS02060 0.44 +0.2 424,336 + BWI76_RS02060 0.44 -0.4 424,336 + BWI76_RS02060 0.44 +0.0 424,336 + BWI76_RS02060 0.44 +0.4 424,336 + BWI76_RS02060 0.44 -0.3 424,336 + BWI76_RS02060 0.44 -0.2 424,336 + BWI76_RS02060 0.44 +0.2 424,337 + BWI76_RS02060 0.45 +0.5 424,337 + BWI76_RS02060 0.45 -1.5 424,344 - BWI76_RS02060 0.46 -0.5 424,345 - BWI76_RS02060 0.46 +0.3 424,348 + BWI76_RS02060 0.47 -0.1 424,348 + BWI76_RS02060 0.47 -0.5 424,348 + BWI76_RS02060 0.47 -0.3 424,348 + BWI76_RS02060 0.47 -0.5 424,348 + BWI76_RS02060 0.47 +4.0 424,348 + BWI76_RS02060 0.47 +0.2 424,348 + BWI76_RS02060 0.47 +0.5 424,348 + BWI76_RS02060 0.47 +0.2 424,348 + BWI76_RS02060 0.47 -0.0 424,348 + BWI76_RS02060 0.47 -0.1 424,348 + BWI76_RS02060 0.47 +0.4 424,348 + BWI76_RS02060 0.47 -0.1 424,348 + BWI76_RS02060 0.47 +0.5 424,348 + BWI76_RS02060 0.47 +0.1 424,348 + BWI76_RS02060 0.47 -0.4 424,349 - BWI76_RS02060 0.47 +0.5 424,356 - BWI76_RS02060 0.48 -0.8 424,356 - BWI76_RS02060 0.48 -1.2 424,356 - BWI76_RS02060 0.48 +0.7 424,358 + BWI76_RS02060 0.48 -1.4 424,358 + BWI76_RS02060 0.48 +0.5 424,358 + BWI76_RS02060 0.48 +0.2 424,361 - BWI76_RS02060 0.49 -2.7 424,425 + BWI76_RS02060 0.61 -1.3 424,425 + BWI76_RS02060 0.61 -1.0 424,425 + BWI76_RS02060 0.61 +0.5 424,433 + BWI76_RS02060 0.62 +0.1 424,433 + BWI76_RS02060 0.62 +0.2 424,433 + BWI76_RS02060 0.62 -1.8 424,433 + BWI76_RS02060 0.62 +0.5 424,433 + BWI76_RS02060 0.62 +1.5 424,441 - BWI76_RS02060 0.64 +0.1
Or see this region's nucleotide sequence