Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01280 and BWI76_RS01285 are separated by 165 nucleotides BWI76_RS01285 and BWI76_RS01290 are separated by 3 nucleotides BWI76_RS01290 and BWI76_RS01295 are separated by 267 nucleotides
BWI76_RS01280: BWI76_RS01280 - 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase, at 261,981 to 264,242
_RS01280
BWI76_RS01285: BWI76_RS01285 - amino acid ABC transporter substrate-binding protein, at 264,408 to 265,097
_RS01285
BWI76_RS01290: BWI76_RS01290 - putative dienelactone hydrolase, at 265,101 to 265,913
_RS01290
BWI76_RS01295: BWI76_RS01295 - Uridine phosphorylase (EC 2.4.2.3) <small>(from data)</small>, at 266,181 to 266,942
_RS01295
Position (kb) 265
266 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 264.104 kb on + strand at 264.142 kb on + strand at 264.217 kb on - strand at 264.217 kb on - strand at 264.217 kb on - strand at 264.319 kb on - strand at 264.367 kb on - strand at 264.448 kb on + strand at 264.505 kb on + strand, within BWI76_RS01285 at 264.568 kb on - strand, within BWI76_RS01285 at 264.662 kb on - strand, within BWI76_RS01285 at 264.761 kb on - strand, within BWI76_RS01285 at 264.958 kb on + strand, within BWI76_RS01285 at 264.992 kb on + strand, within BWI76_RS01285 at 265.007 kb on - strand, within BWI76_RS01285 at 265.060 kb on - strand at 265.099 kb on - strand at 265.132 kb on - strand at 265.279 kb on + strand, within BWI76_RS01290 at 265.420 kb on + strand, within BWI76_RS01290 at 265.420 kb on + strand, within BWI76_RS01290 at 265.444 kb on + strand, within BWI76_RS01290 at 265.468 kb on + strand, within BWI76_RS01290 at 265.545 kb on - strand, within BWI76_RS01290 at 265.846 kb on - strand at 265.961 kb on + strand at 265.961 kb on + strand at 266.033 kb on - strand at 266.233 kb on + strand at 266.251 kb on + strand at 266.354 kb on - strand, within BWI76_RS01295 at 266.362 kb on - strand at 266.423 kb on + strand, within BWI76_RS01295 at 266.533 kb on + strand, within BWI76_RS01295 at 266.600 kb on + strand, within BWI76_RS01295 at 266.600 kb on + strand, within BWI76_RS01295 at 266.670 kb on - strand, within BWI76_RS01295 at 266.678 kb on + strand, within BWI76_RS01295
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 264,104 + +0.4 264,142 + +0.5 264,217 - +0.2 264,217 - -0.3 264,217 - +0.3 264,319 - +0.3 264,367 - -0.3 264,448 + -1.1 264,505 + BWI76_RS01285 0.14 -0.3 264,568 - BWI76_RS01285 0.23 +1.3 264,662 - BWI76_RS01285 0.37 -0.6 264,761 - BWI76_RS01285 0.51 +0.4 264,958 + BWI76_RS01285 0.80 +0.7 264,992 + BWI76_RS01285 0.85 +0.5 265,007 - BWI76_RS01285 0.87 -0.4 265,060 - -0.9 265,099 - +0.4 265,132 - -0.4 265,279 + BWI76_RS01290 0.22 -0.5 265,420 + BWI76_RS01290 0.39 -0.2 265,420 + BWI76_RS01290 0.39 -0.9 265,444 + BWI76_RS01290 0.42 -2.0 265,468 + BWI76_RS01290 0.45 -0.3 265,545 - BWI76_RS01290 0.55 -2.9 265,846 - -0.7 265,961 + -0.7 265,961 + +0.3 266,033 - -0.1 266,233 + +1.4 266,251 + -0.8 266,354 - BWI76_RS01295 0.23 +0.6 266,362 - -1.2 266,423 + BWI76_RS01295 0.32 +0.2 266,533 + BWI76_RS01295 0.46 +1.5 266,600 + BWI76_RS01295 0.55 -0.2 266,600 + BWI76_RS01295 0.55 -0.4 266,670 - BWI76_RS01295 0.64 +0.2 266,678 + BWI76_RS01295 0.65 +0.0
Or see this region's nucleotide sequence