Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22645 and BWI76_RS22650 are separated by 158 nucleotides BWI76_RS22650 and BWI76_RS22655 are separated by 122 nucleotides BWI76_RS22655 and BWI76_RS22660 overlap by 7 nucleotides
BWI76_RS22645: BWI76_RS22645 - RNA polymerase sigma factor RpoS, at 4,720,103 to 4,721,095
_RS22645
BWI76_RS22650: BWI76_RS22650 - lipoprotein NlpD, at 4,721,254 to 4,722,372
_RS22650
BWI76_RS22655: BWI76_RS22655 - protein-L-isoaspartate O-methyltransferase, at 4,722,495 to 4,723,121
_RS22655
BWI76_RS22660: BWI76_RS22660 - 5'/3'-nucleotidase SurE, at 4,723,115 to 4,723,876
_RS22660
Position (kb)
4721
4722
4723 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4720.258 kb on + strand, within BWI76_RS22645 at 4720.279 kb on - strand, within BWI76_RS22645 at 4720.785 kb on - strand, within BWI76_RS22645 at 4720.919 kb on - strand, within BWI76_RS22645 at 4721.005 kb on - strand at 4721.125 kb on - strand at 4721.329 kb on + strand at 4721.337 kb on - strand at 4721.337 kb on - strand at 4721.344 kb on + strand at 4721.347 kb on - strand at 4721.475 kb on + strand, within BWI76_RS22650 at 4721.526 kb on + strand, within BWI76_RS22650 at 4721.746 kb on + strand, within BWI76_RS22650 at 4721.746 kb on + strand, within BWI76_RS22650 at 4722.010 kb on - strand, within BWI76_RS22650 at 4722.040 kb on - strand, within BWI76_RS22650 at 4722.093 kb on - strand, within BWI76_RS22650 at 4722.199 kb on + strand, within BWI76_RS22650 at 4722.242 kb on + strand, within BWI76_RS22650 at 4722.308 kb on - strand at 4722.378 kb on - strand at 4722.421 kb on + strand at 4722.775 kb on - strand, within BWI76_RS22655 at 4722.799 kb on + strand, within BWI76_RS22655 at 4722.807 kb on - strand, within BWI76_RS22655 at 4722.807 kb on - strand, within BWI76_RS22655 at 4722.901 kb on - strand, within BWI76_RS22655 at 4722.933 kb on + strand, within BWI76_RS22655 at 4722.941 kb on - strand at 4723.281 kb on + strand, within BWI76_RS22660 at 4723.289 kb on - strand, within BWI76_RS22660 at 4723.368 kb on + strand, within BWI76_RS22660
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X remove 4,720,258 + BWI76_RS22645 0.16 -1.9 4,720,279 - BWI76_RS22645 0.18 -2.4 4,720,785 - BWI76_RS22645 0.69 -0.4 4,720,919 - BWI76_RS22645 0.82 -1.6 4,721,005 - -0.9 4,721,125 - -0.3 4,721,329 + +1.1 4,721,337 - -1.3 4,721,337 - -1.3 4,721,344 + -2.5 4,721,347 - -2.8 4,721,475 + BWI76_RS22650 0.20 -3.9 4,721,526 + BWI76_RS22650 0.24 -1.9 4,721,746 + BWI76_RS22650 0.44 -1.4 4,721,746 + BWI76_RS22650 0.44 -2.8 4,722,010 - BWI76_RS22650 0.68 -2.0 4,722,040 - BWI76_RS22650 0.70 -2.0 4,722,093 - BWI76_RS22650 0.75 -1.6 4,722,199 + BWI76_RS22650 0.84 -4.6 4,722,242 + BWI76_RS22650 0.88 -1.1 4,722,308 - -1.2 4,722,378 - -0.2 4,722,421 + +0.8 4,722,775 - BWI76_RS22655 0.45 -0.0 4,722,799 + BWI76_RS22655 0.48 -0.6 4,722,807 - BWI76_RS22655 0.50 -0.3 4,722,807 - BWI76_RS22655 0.50 -1.1 4,722,901 - BWI76_RS22655 0.65 +0.1 4,722,933 + BWI76_RS22655 0.70 -0.2 4,722,941 - +1.6 4,723,281 + BWI76_RS22660 0.22 -0.3 4,723,289 - BWI76_RS22660 0.23 -0.5 4,723,368 + BWI76_RS22660 0.33 -0.7
Or see this region's nucleotide sequence