Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04505 and BWI76_RS04510 are separated by 40 nucleotides BWI76_RS04510 and BWI76_RS04515 are separated by 4 nucleotides BWI76_RS04515 and BWI76_RS04520 are separated by 2 nucleotides BWI76_RS04520 and BWI76_RS04525 overlap by 4 nucleotides
BWI76_RS04505: BWI76_RS04505 - dihydrofolate reductase, at 932,871 to 933,350
_RS04505
BWI76_RS04510: BWI76_RS04510 - bis(5'-nucleosyl)-tetraphosphatase (symmetrical), at 933,391 to 934,239
_RS04510
BWI76_RS04515: BWI76_RS04515 - Co2+/Mg2+ efflux protein ApaG, at 934,244 to 934,621
_RS04515
BWI76_RS04520: BWI76_RS04520 - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase, at 934,624 to 935,445
_RS04520
BWI76_RS04525: BWI76_RS04525 - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 935,442 to 936,431
_RS04525
Position (kb) 934
935 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 933.478 kb on + strand, within BWI76_RS04510 at 933.624 kb on + strand, within BWI76_RS04510 at 934.061 kb on - strand, within BWI76_RS04510 at 934.344 kb on - strand, within BWI76_RS04515 at 934.605 kb on - strand at 935.290 kb on + strand, within BWI76_RS04520 at 935.422 kb on + strand at 935.511 kb on + strand at 935.533 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X remove 933,478 + BWI76_RS04510 0.10 -3.2 933,624 + BWI76_RS04510 0.27 -0.3 934,061 - BWI76_RS04510 0.79 -2.0 934,344 - BWI76_RS04515 0.26 -1.3 934,605 - -2.1 935,290 + BWI76_RS04520 0.81 -1.5 935,422 + +0.4 935,511 + +0.0 935,533 - +0.0
Or see this region's nucleotide sequence