Experiment: Levan - from Erwinia herbicola; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1749 and BT1750 are separated by 54 nucleotides BT1750 and BT1751 overlap by 4 nucleotides BT1751 and BT1752 are separated by 604 nucleotides
BT1749: BT1749 - glycine betaine-binding protein precursor (NCBI ptt file), at 2,159,736 to 2,160,569
BT1749
BT1750: BT1750 - glycine betaine/L-proline transport system permease (NCBI ptt file), at 2,160,624 to 2,161,454
BT1750
BT1751: BT1751 - Glycine betaine transport ATP-binding protein (NCBI ptt file), at 2,161,451 to 2,162,677
BT1751
BT1752: BT1752 - hypothetical protein (NCBI ptt file), at 2,163,282 to 2,164,436
BT1752
Position (kb)
2161
2162
2163 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2160.505 kb on - strand at 2160.553 kb on - strand at 2160.590 kb on + strand at 2160.591 kb on - strand at 2160.591 kb on - strand at 2160.591 kb on - strand at 2160.601 kb on + strand at 2160.798 kb on + strand, within BT1750 at 2160.798 kb on + strand, within BT1750 at 2160.848 kb on + strand, within BT1750 at 2160.896 kb on + strand, within BT1750 at 2160.921 kb on + strand, within BT1750 at 2160.924 kb on + strand, within BT1750 at 2161.098 kb on + strand, within BT1750 at 2161.099 kb on - strand, within BT1750 at 2161.106 kb on + strand, within BT1750 at 2161.179 kb on + strand, within BT1750 at 2161.180 kb on - strand, within BT1750 at 2161.237 kb on - strand, within BT1750 at 2161.254 kb on + strand, within BT1750 at 2161.254 kb on + strand, within BT1750 at 2161.255 kb on - strand, within BT1750 at 2161.277 kb on + strand, within BT1750 at 2161.336 kb on - strand, within BT1750 at 2161.336 kb on - strand, within BT1750 at 2161.353 kb on + strand, within BT1750 at 2161.418 kb on + strand at 2161.419 kb on - strand at 2161.454 kb on + strand at 2161.455 kb on - strand at 2161.460 kb on + strand at 2161.538 kb on + strand at 2161.538 kb on + strand at 2161.609 kb on + strand, within BT1751 at 2161.655 kb on + strand, within BT1751 at 2161.658 kb on + strand, within BT1751 at 2161.834 kb on + strand, within BT1751 at 2161.859 kb on + strand, within BT1751 at 2161.877 kb on + strand, within BT1751 at 2161.902 kb on + strand, within BT1751 at 2161.902 kb on + strand, within BT1751 at 2161.902 kb on + strand, within BT1751 at 2161.949 kb on + strand, within BT1751 at 2161.972 kb on + strand, within BT1751 at 2162.195 kb on + strand, within BT1751 at 2162.195 kb on + strand, within BT1751 at 2162.273 kb on + strand, within BT1751 at 2162.282 kb on + strand, within BT1751 at 2162.306 kb on - strand, within BT1751 at 2162.440 kb on + strand, within BT1751 at 2162.560 kb on + strand at 2162.561 kb on - strand at 2162.667 kb on - strand at 2162.671 kb on + strand at 2162.683 kb on - strand at 2162.752 kb on + strand at 2162.752 kb on + strand at 2162.839 kb on - strand at 2162.903 kb on + strand at 2162.929 kb on - strand at 2162.947 kb on + strand at 2162.948 kb on - strand at 2163.012 kb on + strand at 2163.117 kb on + strand at 2163.119 kb on + strand at 2163.119 kb on + strand at 2163.254 kb on - strand at 2163.295 kb on - strand at 2163.320 kb on - strand at 2163.339 kb on - strand at 2163.493 kb on + strand, within BT1752 at 2163.677 kb on + strand, within BT1752
Per-strain Table
Position Strand Gene LocusTag Fraction Levan - from Erwinia herbicola; autoclaved 2.5 mg/ml (C) remove 2,160,505 - -0.4 2,160,553 - -0.7 2,160,590 + +2.3 2,160,591 - +1.0 2,160,591 - -1.1 2,160,591 - -1.0 2,160,601 + -0.6 2,160,798 + BT1750 0.21 -0.4 2,160,798 + BT1750 0.21 -1.3 2,160,848 + BT1750 0.27 -2.0 2,160,896 + BT1750 0.33 +0.5 2,160,921 + BT1750 0.36 +1.6 2,160,924 + BT1750 0.36 -1.2 2,161,098 + BT1750 0.57 -2.7 2,161,099 - BT1750 0.57 -0.0 2,161,106 + BT1750 0.58 -1.0 2,161,179 + BT1750 0.67 +0.6 2,161,180 - BT1750 0.67 -1.6 2,161,237 - BT1750 0.74 -1.0 2,161,254 + BT1750 0.76 -1.9 2,161,254 + BT1750 0.76 -2.0 2,161,255 - BT1750 0.76 -0.0 2,161,277 + BT1750 0.79 -0.9 2,161,336 - BT1750 0.86 -0.9 2,161,336 - BT1750 0.86 -0.0 2,161,353 + BT1750 0.88 -1.0 2,161,418 + -0.8 2,161,419 - -0.0 2,161,454 + +1.0 2,161,455 - -0.0 2,161,460 + -1.0 2,161,538 + -0.6 2,161,538 + -1.7 2,161,609 + BT1751 0.13 -0.2 2,161,655 + BT1751 0.17 -1.6 2,161,658 + BT1751 0.17 +1.2 2,161,834 + BT1751 0.31 -1.4 2,161,859 + BT1751 0.33 -1.6 2,161,877 + BT1751 0.35 -1.8 2,161,902 + BT1751 0.37 +1.0 2,161,902 + BT1751 0.37 -1.2 2,161,902 + BT1751 0.37 -0.4 2,161,949 + BT1751 0.41 -2.0 2,161,972 + BT1751 0.42 +1.0 2,162,195 + BT1751 0.61 -1.2 2,162,195 + BT1751 0.61 -0.6 2,162,273 + BT1751 0.67 -1.4 2,162,282 + BT1751 0.68 +0.7 2,162,306 - BT1751 0.70 +1.0 2,162,440 + BT1751 0.81 -1.1 2,162,560 + -1.0 2,162,561 - -1.1 2,162,667 - +0.4 2,162,671 + -0.0 2,162,683 - -1.0 2,162,752 + -1.6 2,162,752 + -1.0 2,162,839 - -0.0 2,162,903 + -0.0 2,162,929 - -0.0 2,162,947 + -0.5 2,162,948 - -0.0 2,163,012 + +0.4 2,163,117 + -2.3 2,163,119 + -1.0 2,163,119 + -2.2 2,163,254 - -1.6 2,163,295 - -1.0 2,163,320 - -0.0 2,163,339 - -1.6 2,163,493 + BT1752 0.18 -2.6 2,163,677 + BT1752 0.34 -0.0
Or see this region's nucleotide sequence