Experiment: Levan - from Erwinia herbicola; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0102 and BT0103 are separated by 173 nucleotides BT0103 and BT0104 overlap by 4 nucleotides BT0104 and BT0105 overlap by 4 nucleotides
BT0102: BT0102 - conserved hypothetical protein (NCBI ptt file), at 92,986 to 94,995
BT0102
BT0103: BT0103 - conserved hypothetical protein (NCBI ptt file), at 95,169 to 96,704
BT0103
BT0104: BT0104 - hypothetical protein (NCBI ptt file), at 96,701 to 96,889
BT0104
BT0105: BT0105 - DNA topoisomerase I (Omega-protein) (NCBI ptt file), at 96,886 to 98,967
BT0105
Position (kb) 95
96
97 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 94.270 kb on - strand, within BT0102 at 94.315 kb on - strand, within BT0102 at 94.442 kb on + strand, within BT0102 at 94.635 kb on + strand, within BT0102 at 94.636 kb on - strand, within BT0102 at 94.816 kb on + strand at 94.976 kb on + strand at 95.057 kb on - strand at 95.074 kb on - strand at 95.111 kb on + strand at 95.112 kb on - strand at 95.231 kb on - strand at 95.406 kb on - strand, within BT0103 at 95.644 kb on - strand, within BT0103 at 95.644 kb on - strand, within BT0103 at 95.644 kb on - strand, within BT0103 at 95.644 kb on - strand, within BT0103 at 95.695 kb on + strand, within BT0103 at 95.748 kb on + strand, within BT0103 at 96.140 kb on - strand, within BT0103 at 96.332 kb on - strand, within BT0103 at 96.332 kb on - strand, within BT0103 at 96.337 kb on + strand, within BT0103 at 96.417 kb on + strand, within BT0103 at 96.418 kb on - strand, within BT0103 at 96.426 kb on + strand, within BT0103 at 96.429 kb on - strand, within BT0103 at 96.830 kb on + strand, within BT0104 at 96.883 kb on - strand at 97.075 kb on - strand at 97.161 kb on - strand, within BT0105 at 97.161 kb on - strand, within BT0105 at 97.199 kb on + strand, within BT0105 at 97.406 kb on + strand, within BT0105 at 97.422 kb on + strand, within BT0105 at 97.423 kb on - strand, within BT0105 at 97.499 kb on + strand, within BT0105 at 97.500 kb on - strand, within BT0105 at 97.500 kb on - strand, within BT0105 at 97.543 kb on + strand, within BT0105
Per-strain Table
Position Strand Gene LocusTag Fraction Levan - from Erwinia herbicola; autoclaved 2.5 mg/ml (C) remove 94,270 - BT0102 0.64 -1.6 94,315 - BT0102 0.66 +0.0 94,442 + BT0102 0.72 -1.6 94,635 + BT0102 0.82 -1.0 94,636 - BT0102 0.82 -1.6 94,816 + -2.8 94,976 + -1.6 95,057 - -1.1 95,074 - -1.3 95,111 + -1.6 95,112 - -0.6 95,231 - -1.0 95,406 - BT0103 0.15 -2.3 95,644 - BT0103 0.31 -2.6 95,644 - BT0103 0.31 -1.6 95,644 - BT0103 0.31 +0.0 95,644 - BT0103 0.31 -2.0 95,695 + BT0103 0.34 -0.6 95,748 + BT0103 0.38 +0.0 96,140 - BT0103 0.63 -2.3 96,332 - BT0103 0.76 -1.6 96,332 - BT0103 0.76 -0.5 96,337 + BT0103 0.76 -0.8 96,417 + BT0103 0.81 -1.0 96,418 - BT0103 0.81 -2.3 96,426 + BT0103 0.82 +0.0 96,429 - BT0103 0.82 -1.6 96,830 + BT0104 0.68 -1.2 96,883 - -1.0 97,075 - -1.6 97,161 - BT0105 0.13 -0.9 97,161 - BT0105 0.13 +1.0 97,199 + BT0105 0.15 -2.0 97,406 + BT0105 0.25 +0.0 97,422 + BT0105 0.26 -1.4 97,423 - BT0105 0.26 -0.8 97,499 + BT0105 0.29 -1.3 97,500 - BT0105 0.29 -1.4 97,500 - BT0105 0.29 -2.0 97,543 + BT0105 0.32 -0.6
Or see this region's nucleotide sequence