Experiment: a-Cyclodextrin 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SusB and SusA are separated by 196 nucleotides SusA and SusR are separated by 93 nucleotides
BT3703: SusB - alpha-glucosidase SusB (NCBI ptt file), at 4,810,585 to 4,812,801
SusB
BT3704: SusA - SusA: alpha-amylase / neopullulanase / cyclomaltodextrinase (EC 3.2.1.135; EC 3.2.1.54) (from data) , at 4,812,998 to 4,814,851
SusA
BT3705: SusR - regulatory protein SusR (NCBI ptt file), at 4,814,945 to 4,816,693
SusR
Position (kb)
4812
4813
4814
4815 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 4812.015 kb on + strand, within SusB at 4812.015 kb on + strand, within SusB at 4812.042 kb on + strand, within SusB at 4812.042 kb on + strand, within SusB at 4812.083 kb on + strand, within SusB at 4812.084 kb on - strand, within SusB at 4812.121 kb on + strand, within SusB at 4812.121 kb on + strand, within SusB at 4812.212 kb on + strand, within SusB at 4812.213 kb on - strand, within SusB at 4812.233 kb on + strand, within SusB at 4812.233 kb on + strand, within SusB at 4812.234 kb on - strand at 4812.385 kb on - strand, within SusB at 4812.431 kb on + strand, within SusB at 4812.592 kb on - strand at 4812.652 kb on - strand at 4812.703 kb on + strand at 4812.805 kb on - strand at 4812.901 kb on - strand at 4812.954 kb on - strand at 4812.954 kb on - strand at 4812.996 kb on + strand at 4813.074 kb on - strand at 4813.172 kb on - strand at 4813.431 kb on + strand, within SusA at 4813.434 kb on - strand, within SusA at 4813.460 kb on + strand, within SusA at 4813.461 kb on - strand, within SusA at 4813.502 kb on - strand, within SusA at 4813.502 kb on - strand, within SusA at 4813.593 kb on - strand, within SusA at 4813.778 kb on + strand, within SusA at 4813.779 kb on - strand, within SusA at 4813.834 kb on + strand, within SusA at 4813.835 kb on - strand, within SusA at 4813.835 kb on - strand, within SusA at 4813.921 kb on - strand, within SusA at 4814.056 kb on - strand, within SusA at 4814.107 kb on - strand, within SusA at 4814.107 kb on - strand, within SusA at 4814.179 kb on + strand, within SusA at 4814.250 kb on - strand, within SusA at 4814.297 kb on + strand, within SusA at 4814.298 kb on - strand, within SusA at 4814.298 kb on - strand, within SusA at 4814.299 kb on + strand, within SusA at 4814.321 kb on + strand, within SusA at 4814.321 kb on + strand, within SusA at 4814.322 kb on - strand, within SusA at 4814.322 kb on - strand, within SusA at 4814.326 kb on - strand, within SusA at 4814.461 kb on + strand, within SusA at 4814.611 kb on - strand, within SusA at 4814.697 kb on - strand at 4814.712 kb on - strand at 4814.714 kb on + strand at 4814.718 kb on - strand at 4814.771 kb on - strand at 4814.793 kb on - strand at 4815.054 kb on - strand at 4815.054 kb on - strand at 4815.066 kb on - strand at 4815.106 kb on - strand at 4815.110 kb on + strand at 4815.111 kb on - strand at 4815.164 kb on - strand, within SusR at 4815.205 kb on + strand, within SusR at 4815.205 kb on + strand, within SusR at 4815.207 kb on + strand, within SusR at 4815.207 kb on + strand, within SusR at 4815.208 kb on - strand, within SusR at 4815.208 kb on - strand, within SusR at 4815.208 kb on - strand, within SusR at 4815.208 kb on - strand, within SusR at 4815.231 kb on + strand, within SusR at 4815.250 kb on + strand, within SusR at 4815.308 kb on + strand, within SusR at 4815.308 kb on + strand, within SusR at 4815.308 kb on + strand, within SusR at 4815.309 kb on - strand, within SusR at 4815.362 kb on + strand, within SusR at 4815.365 kb on - strand, within SusR at 4815.384 kb on + strand, within SusR at 4815.397 kb on - strand, within SusR at 4815.417 kb on - strand, within SusR at 4815.417 kb on - strand, within SusR at 4815.454 kb on - strand, within SusR at 4815.465 kb on - strand, within SusR at 4815.465 kb on - strand, within SusR at 4815.476 kb on - strand, within SusR at 4815.534 kb on - strand, within SusR at 4815.679 kb on + strand, within SusR at 4815.695 kb on - strand, within SusR at 4815.695 kb on - strand, within SusR at 4815.775 kb on - strand, within SusR at 4815.775 kb on - strand, within SusR at 4815.779 kb on - strand, within SusR at 4815.782 kb on + strand, within SusR at 4815.783 kb on - strand, within SusR at 4815.783 kb on - strand, within SusR at 4815.783 kb on - strand, within SusR at 4815.804 kb on - strand, within SusR at 4815.805 kb on + strand, within SusR
Per-strain Table
Position Strand Gene LocusTag Fraction a-Cyclodextrin 2.5 mg/ml (C) remove 4,812,015 + SusB BT3703 0.65 -1.7 4,812,015 + SusB BT3703 0.65 -4.6 4,812,042 + SusB BT3703 0.66 -2.9 4,812,042 + SusB BT3703 0.66 -4.3 4,812,083 + SusB BT3703 0.68 -2.4 4,812,084 - SusB BT3703 0.68 -0.1 4,812,121 + SusB BT3703 0.69 -3.5 4,812,121 + SusB BT3703 0.69 -0.1 4,812,212 + SusB BT3703 0.73 -2.1 4,812,213 - SusB BT3703 0.73 -1.1 4,812,233 + SusB BT3703 0.74 -3.3 4,812,233 + SusB BT3703 0.74 -1.1 4,812,234 - -2.4 4,812,385 - SusB BT3703 0.81 -2.4 4,812,431 + SusB BT3703 0.83 -3.3 4,812,592 - -1.7 4,812,652 - -2.7 4,812,703 + -3.1 4,812,805 - -4.3 4,812,901 - -0.1 4,812,954 - -1.7 4,812,954 - +0.9 4,812,996 + -1.9 4,813,074 - -3.3 4,813,172 - -1.7 4,813,431 + SusA BT3704 0.23 -1.1 4,813,434 - SusA BT3704 0.24 -2.4 4,813,460 + SusA BT3704 0.25 -2.4 4,813,461 - SusA BT3704 0.25 -4.6 4,813,502 - SusA BT3704 0.27 -0.1 4,813,502 - SusA BT3704 0.27 -1.7 4,813,593 - SusA BT3704 0.32 -1.1 4,813,778 + SusA BT3704 0.42 -1.7 4,813,779 - SusA BT3704 0.42 -1.8 4,813,834 + SusA BT3704 0.45 -1.7 4,813,835 - SusA BT3704 0.45 -2.7 4,813,835 - SusA BT3704 0.45 -1.1 4,813,921 - SusA BT3704 0.50 -1.1 4,814,056 - SusA BT3704 0.57 -4.2 4,814,107 - SusA BT3704 0.60 -2.9 4,814,107 - SusA BT3704 0.60 -0.1 4,814,179 + SusA BT3704 0.64 -2.7 4,814,250 - SusA BT3704 0.68 -3.7 4,814,297 + SusA BT3704 0.70 -1.1 4,814,298 - SusA BT3704 0.70 -5.1 4,814,298 - SusA BT3704 0.70 -2.9 4,814,299 + SusA BT3704 0.70 -0.1 4,814,321 + SusA BT3704 0.71 -1.1 4,814,321 + SusA BT3704 0.71 -2.1 4,814,322 - SusA BT3704 0.71 -1.1 4,814,322 - SusA BT3704 0.71 -2.1 4,814,326 - SusA BT3704 0.72 -2.8 4,814,461 + SusA BT3704 0.79 -2.7 4,814,611 - SusA BT3704 0.87 -1.1 4,814,697 - -4.0 4,814,712 - -0.1 4,814,714 + -2.9 4,814,718 - -3.3 4,814,771 - -1.1 4,814,793 - -2.9 4,815,054 - -4.4 4,815,054 - -1.7 4,815,066 - -0.1 4,815,106 - -3.2 4,815,110 + -1.7 4,815,111 - -2.4 4,815,164 - SusR BT3705 0.13 -0.1 4,815,205 + SusR BT3705 0.15 -3.1 4,815,205 + SusR BT3705 0.15 -1.7 4,815,207 + SusR BT3705 0.15 -0.1 4,815,207 + SusR BT3705 0.15 -2.7 4,815,208 - SusR BT3705 0.15 +2.7 4,815,208 - SusR BT3705 0.15 -1.7 4,815,208 - SusR BT3705 0.15 -1.7 4,815,208 - SusR BT3705 0.15 +0.9 4,815,231 + SusR BT3705 0.16 -5.5 4,815,250 + SusR BT3705 0.17 -5.5 4,815,308 + SusR BT3705 0.21 -0.1 4,815,308 + SusR BT3705 0.21 -2.5 4,815,308 + SusR BT3705 0.21 -0.1 4,815,309 - SusR BT3705 0.21 -2.1 4,815,362 + SusR BT3705 0.24 -2.4 4,815,365 - SusR BT3705 0.24 -3.2 4,815,384 + SusR BT3705 0.25 -3.4 4,815,397 - SusR BT3705 0.26 -5.1 4,815,417 - SusR BT3705 0.27 -0.1 4,815,417 - SusR BT3705 0.27 -1.1 4,815,454 - SusR BT3705 0.29 -2.9 4,815,465 - SusR BT3705 0.30 -5.3 4,815,465 - SusR BT3705 0.30 -3.1 4,815,476 - SusR BT3705 0.30 -3.7 4,815,534 - SusR BT3705 0.34 -2.7 4,815,679 + SusR BT3705 0.42 -3.7 4,815,695 - SusR BT3705 0.43 -1.1 4,815,695 - SusR BT3705 0.43 -6.1 4,815,775 - SusR BT3705 0.47 -4.0 4,815,775 - SusR BT3705 0.47 -0.1 4,815,779 - SusR BT3705 0.48 -2.4 4,815,782 + SusR BT3705 0.48 -2.4 4,815,783 - SusR BT3705 0.48 -2.1 4,815,783 - SusR BT3705 0.48 -2.4 4,815,783 - SusR BT3705 0.48 -1.7 4,815,804 - SusR BT3705 0.49 -5.1 4,815,805 + SusR BT3705 0.49 -4.6
Or see this region's nucleotide sequence