Experiment: Amylopectin from Potato; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2549 and BT2550 are separated by 10 nucleotides BT2550 and BT2551 are separated by 179 nucleotides BT2551 and BT2552 are separated by 30 nucleotides
BT2549: BT2549 - putative carboxypeptidase (NCBI ptt file), at 3,181,823 to 3,182,731
BT2549
BT2550: BT2550 - beta-glucanase precursor (NCBI ptt file), at 3,182,742 to 3,183,548
BT2550
BT2551: BT2551 - phosphate butyryltransferase (NCBI ptt file), at 3,183,728 to 3,184,672
BT2551
BT2552: BT2552 - putative butyrate kinase (NCBI ptt file), at 3,184,703 to 3,185,764
BT2552
Position (kb)
3183
3184
3185 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3182.736 kb on - strand at 3182.861 kb on - strand, within BT2550 at 3182.905 kb on - strand, within BT2550 at 3182.924 kb on + strand, within BT2550 at 3182.924 kb on + strand, within BT2550 at 3182.925 kb on - strand, within BT2550 at 3182.928 kb on + strand, within BT2550 at 3182.929 kb on - strand, within BT2550 at 3182.929 kb on - strand, within BT2550 at 3182.987 kb on - strand, within BT2550 at 3183.083 kb on + strand, within BT2550 at 3183.161 kb on + strand, within BT2550 at 3183.242 kb on + strand, within BT2550 at 3183.262 kb on + strand, within BT2550 at 3183.356 kb on + strand, within BT2550 at 3183.358 kb on + strand, within BT2550 at 3183.358 kb on + strand, within BT2550 at 3183.359 kb on - strand, within BT2550 at 3183.359 kb on - strand, within BT2550 at 3183.363 kb on - strand, within BT2550 at 3183.608 kb on + strand at 3183.611 kb on - strand at 3183.631 kb on - strand at 3183.652 kb on + strand at 3183.653 kb on - strand at 3183.820 kb on + strand at 3183.820 kb on + strand at 3183.830 kb on + strand, within BT2551 at 3183.905 kb on + strand, within BT2551 at 3183.932 kb on + strand, within BT2551 at 3183.932 kb on + strand, within BT2551 at 3184.017 kb on + strand, within BT2551 at 3184.017 kb on + strand, within BT2551 at 3184.139 kb on - strand, within BT2551 at 3184.211 kb on + strand, within BT2551 at 3184.266 kb on + strand, within BT2551 at 3184.360 kb on - strand, within BT2551 at 3184.383 kb on + strand, within BT2551 at 3184.383 kb on + strand, within BT2551 at 3184.471 kb on + strand, within BT2551 at 3184.473 kb on + strand, within BT2551 at 3184.493 kb on + strand, within BT2551 at 3184.493 kb on + strand, within BT2551 at 3184.601 kb on + strand at 3184.602 kb on - strand at 3184.676 kb on + strand at 3184.747 kb on - strand at 3184.795 kb on + strand at 3184.796 kb on - strand at 3184.981 kb on + strand, within BT2552 at 3185.116 kb on + strand, within BT2552 at 3185.260 kb on + strand, within BT2552 at 3185.299 kb on + strand, within BT2552 at 3185.407 kb on - strand, within BT2552 at 3185.417 kb on - strand, within BT2552 at 3185.417 kb on - strand, within BT2552 at 3185.531 kb on + strand, within BT2552 at 3185.531 kb on + strand, within BT2552 at 3185.531 kb on + strand, within BT2552 at 3185.532 kb on - strand, within BT2552 at 3185.533 kb on + strand, within BT2552 at 3185.533 kb on + strand, within BT2552 at 3185.534 kb on - strand, within BT2552 at 3185.584 kb on - strand, within BT2552 at 3185.609 kb on - strand, within BT2552 at 3185.624 kb on + strand, within BT2552 at 3185.627 kb on - strand, within BT2552 at 3185.670 kb on + strand at 3185.670 kb on + strand at 3185.670 kb on + strand at 3185.671 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Amylopectin from Potato; autoclaved 2.5 mg/ml (C) remove 3,182,736 - -1.5 3,182,861 - BT2550 0.15 +0.5 3,182,905 - BT2550 0.20 -2.1 3,182,924 + BT2550 0.23 +0.5 3,182,924 + BT2550 0.23 -1.3 3,182,925 - BT2550 0.23 +0.5 3,182,928 + BT2550 0.23 -1.1 3,182,929 - BT2550 0.23 +0.5 3,182,929 - BT2550 0.23 -0.5 3,182,987 - BT2550 0.30 -1.9 3,183,083 + BT2550 0.42 -0.1 3,183,161 + BT2550 0.52 -0.8 3,183,242 + BT2550 0.62 +0.5 3,183,262 + BT2550 0.64 +0.5 3,183,356 + BT2550 0.76 -1.7 3,183,358 + BT2550 0.76 -0.2 3,183,358 + BT2550 0.76 -2.1 3,183,359 - BT2550 0.76 -1.5 3,183,359 - BT2550 0.76 -0.9 3,183,363 - BT2550 0.77 -1.2 3,183,608 + -1.0 3,183,611 - -1.5 3,183,631 - -1.1 3,183,652 + -1.1 3,183,653 - +0.5 3,183,820 + -1.1 3,183,820 + -1.5 3,183,830 + BT2551 0.11 +1.5 3,183,905 + BT2551 0.19 -2.5 3,183,932 + BT2551 0.22 -1.9 3,183,932 + BT2551 0.22 -1.1 3,184,017 + BT2551 0.31 +1.5 3,184,017 + BT2551 0.31 -0.6 3,184,139 - BT2551 0.43 -1.1 3,184,211 + BT2551 0.51 +1.5 3,184,266 + BT2551 0.57 -0.5 3,184,360 - BT2551 0.67 +0.5 3,184,383 + BT2551 0.69 -0.1 3,184,383 + BT2551 0.69 -1.1 3,184,471 + BT2551 0.79 -1.5 3,184,473 + BT2551 0.79 -1.5 3,184,493 + BT2551 0.81 -1.1 3,184,493 + BT2551 0.81 -1.3 3,184,601 + +0.5 3,184,602 - -2.1 3,184,676 + -1.1 3,184,747 - -2.3 3,184,795 + +0.5 3,184,796 - -1.1 3,184,981 + BT2552 0.26 -0.5 3,185,116 + BT2552 0.39 +0.5 3,185,260 + BT2552 0.52 -0.5 3,185,299 + BT2552 0.56 -1.9 3,185,407 - BT2552 0.66 -1.4 3,185,417 - BT2552 0.67 -1.9 3,185,417 - BT2552 0.67 -1.1 3,185,531 + BT2552 0.78 -1.3 3,185,531 + BT2552 0.78 +1.5 3,185,531 + BT2552 0.78 -0.5 3,185,532 - BT2552 0.78 -0.5 3,185,533 + BT2552 0.78 -1.1 3,185,533 + BT2552 0.78 +0.5 3,185,534 - BT2552 0.78 +0.5 3,185,584 - BT2552 0.83 -0.2 3,185,609 - BT2552 0.85 -3.6 3,185,624 + BT2552 0.87 -1.1 3,185,627 - BT2552 0.87 -1.9 3,185,670 + -2.1 3,185,670 + -2.0 3,185,670 + -1.7 3,185,671 - -0.5
Or see this region's nucleotide sequence