Experiment: Mannan from Saccharomyces cerevisiae; filtered 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1676 and BT1677 are separated by 30 nucleotides BT1677 and BT1678 overlap by 20 nucleotides BT1678 and BT1679 are separated by 7 nucleotides BT1679 and BT1680 are separated by 2 nucleotides
BT1676: BT1676 - Maf protein (NCBI ptt file), at 2,069,981 to 2,070,562
BT1676
BT1677: BT1677 - putative hydrolase (NCBI ptt file), at 2,070,593 to 2,071,114
BT1677
BT1678: BT1678 - conserved hypothetical protein (NCBI ptt file), at 2,071,095 to 2,071,889
BT1678
BT1679: BT1679 - hypothetical protein (NCBI ptt file), at 2,071,897 to 2,072,232
BT1679
BT1680: BT1680 - putative NADH dehydrogenase/NAD(P)H nitroreductase (NCBI ptt file), at 2,072,235 to 2,072,771
BT1680
Position (kb)
2071
2072 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2070.121 kb on - strand, within BT1676 at 2070.132 kb on + strand, within BT1676 at 2070.188 kb on - strand, within BT1676 at 2070.188 kb on - strand, within BT1676 at 2070.214 kb on + strand, within BT1676 at 2070.331 kb on + strand, within BT1676 at 2070.403 kb on - strand, within BT1676 at 2070.474 kb on + strand, within BT1676 at 2070.475 kb on - strand, within BT1676 at 2070.531 kb on - strand at 2070.531 kb on - strand at 2070.532 kb on + strand at 2070.533 kb on - strand at 2070.576 kb on - strand at 2070.587 kb on + strand at 2072.120 kb on - strand, within BT1679 at 2072.143 kb on - strand, within BT1679 at 2072.200 kb on - strand at 2072.212 kb on - strand at 2072.470 kb on - strand, within BT1680 at 2072.487 kb on - strand, within BT1680 at 2072.640 kb on - strand, within BT1680 at 2072.646 kb on - strand, within BT1680 at 2072.646 kb on - strand, within BT1680 at 2072.707 kb on - strand, within BT1680 at 2072.796 kb on - strand at 2072.796 kb on - strand at 2072.796 kb on - strand at 2072.800 kb on - strand at 2072.826 kb on - strand at 2072.844 kb on - strand at 2072.844 kb on - strand at 2072.861 kb on + strand at 2072.870 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mannan from Saccharomyces cerevisiae; filtered 2.5 mg/ml (C) remove 2,070,121 - BT1676 0.24 -2.0 2,070,132 + BT1676 0.26 -0.6 2,070,188 - BT1676 0.36 -0.8 2,070,188 - BT1676 0.36 -0.8 2,070,214 + BT1676 0.40 -0.0 2,070,331 + BT1676 0.60 +0.4 2,070,403 - BT1676 0.73 -0.5 2,070,474 + BT1676 0.85 -2.4 2,070,475 - BT1676 0.85 -1.0 2,070,531 - +0.4 2,070,531 - -0.9 2,070,532 + +0.7 2,070,533 - -3.0 2,070,576 - +1.0 2,070,587 + -0.0 2,072,120 - BT1679 0.66 -1.9 2,072,143 - BT1679 0.73 -1.6 2,072,200 - +0.4 2,072,212 - -2.7 2,072,470 - BT1680 0.44 +0.5 2,072,487 - BT1680 0.47 +1.6 2,072,640 - BT1680 0.75 -2.6 2,072,646 - BT1680 0.77 +1.3 2,072,646 - BT1680 0.77 -0.0 2,072,707 - BT1680 0.88 -1.0 2,072,796 - -1.6 2,072,796 - -0.0 2,072,796 - -1.3 2,072,800 - -1.3 2,072,826 - -2.6 2,072,844 - +0.4 2,072,844 - -2.0 2,072,861 + -1.0 2,072,870 + -3.0
Or see this region's nucleotide sequence