Experiment: Mannan from Saccharomyces cerevisiae; filtered 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0112 and BT0113 are separated by 25 nucleotides BT0113 and BT0114 are separated by 2 nucleotides BT0114 and BT0115 are separated by 12 nucleotides BT0115 and BT0116 are separated by 4 nucleotides
BT0112: BT0112 - putative permease (NCBI ptt file), at 104,093 to 104,998
BT0112
BT0113: BT0113 - hypothetical protein (NCBI ptt file), at 105,024 to 105,524
BT0113
BT0114: BT0114 - putative symporter, arsenic resistance membrane protein (NCBI ptt file), at 105,527 to 106,573
BT0114
BT0115: BT0115 - arsenate reductase (NCBI ptt file), at 106,586 to 107,014
BT0115
BT0116: BT0116 - arsenical pump-driving ATPase (NCBI ptt file), at 107,019 to 108,731
BT0116
Position (kb) 105
106
107 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 104.666 kb on - strand, within BT0112 at 104.690 kb on - strand, within BT0112 at 104.693 kb on + strand, within BT0112 at 104.695 kb on + strand, within BT0112 at 104.695 kb on + strand, within BT0112 at 104.723 kb on + strand, within BT0112 at 104.834 kb on + strand, within BT0112 at 104.893 kb on + strand, within BT0112 at 104.999 kb on - strand at 105.018 kb on + strand at 105.195 kb on + strand, within BT0113 at 105.367 kb on + strand, within BT0113 at 105.368 kb on - strand, within BT0113 at 105.383 kb on + strand, within BT0113 at 105.429 kb on + strand, within BT0113 at 105.432 kb on - strand, within BT0113 at 105.432 kb on - strand, within BT0113 at 105.453 kb on + strand, within BT0113 at 105.453 kb on + strand, within BT0113 at 105.460 kb on - strand, within BT0113 at 105.492 kb on + strand at 105.528 kb on + strand at 105.529 kb on - strand at 105.580 kb on + strand at 105.580 kb on + strand at 105.581 kb on - strand at 105.815 kb on - strand, within BT0114 at 105.818 kb on + strand, within BT0114 at 105.935 kb on + strand, within BT0114 at 105.935 kb on + strand, within BT0114 at 105.968 kb on + strand, within BT0114 at 105.971 kb on + strand, within BT0114 at 106.017 kb on - strand, within BT0114 at 106.018 kb on + strand, within BT0114 at 106.094 kb on + strand, within BT0114 at 106.177 kb on + strand, within BT0114 at 106.216 kb on - strand, within BT0114 at 106.268 kb on + strand, within BT0114 at 106.269 kb on - strand, within BT0114 at 106.269 kb on - strand, within BT0114 at 106.272 kb on + strand, within BT0114 at 106.272 kb on + strand, within BT0114 at 106.272 kb on + strand, within BT0114 at 106.273 kb on - strand, within BT0114 at 106.395 kb on + strand, within BT0114 at 106.427 kb on + strand, within BT0114 at 106.427 kb on + strand, within BT0114 at 106.482 kb on + strand at 106.483 kb on - strand at 106.483 kb on - strand at 106.511 kb on - strand at 106.529 kb on - strand at 106.537 kb on - strand at 106.640 kb on + strand, within BT0115 at 106.640 kb on + strand, within BT0115 at 106.747 kb on + strand, within BT0115 at 106.794 kb on + strand, within BT0115 at 106.815 kb on + strand, within BT0115 at 106.877 kb on + strand, within BT0115 at 106.987 kb on + strand at 107.020 kb on + strand at 107.020 kb on + strand at 107.154 kb on + strand at 107.277 kb on + strand, within BT0116 at 107.332 kb on - strand, within BT0116 at 107.494 kb on - strand, within BT0116 at 107.498 kb on - strand, within BT0116
Per-strain Table
Position Strand Gene LocusTag Fraction Mannan from Saccharomyces cerevisiae; filtered 2.5 mg/ml (C) remove 104,666 - BT0112 0.63 -1.6 104,690 - BT0112 0.66 -0.6 104,693 + BT0112 0.66 -0.5 104,695 + BT0112 0.66 -1.6 104,695 + BT0112 0.66 +0.4 104,723 + BT0112 0.70 -2.2 104,834 + BT0112 0.82 -0.0 104,893 + BT0112 0.88 -0.4 104,999 - -1.0 105,018 + -1.0 105,195 + BT0113 0.34 -2.0 105,367 + BT0113 0.68 -3.2 105,368 - BT0113 0.69 -2.0 105,383 + BT0113 0.72 -0.0 105,429 + BT0113 0.81 -2.6 105,432 - BT0113 0.81 -1.2 105,432 - BT0113 0.81 +2.8 105,453 + BT0113 0.86 -0.3 105,453 + BT0113 0.86 -0.0 105,460 - BT0113 0.87 -4.0 105,492 + -2.2 105,528 + -1.6 105,529 - +1.1 105,580 + -1.3 105,580 + -2.2 105,581 - -1.6 105,815 - BT0114 0.28 -0.0 105,818 + BT0114 0.28 -2.7 105,935 + BT0114 0.39 -2.2 105,935 + BT0114 0.39 -0.8 105,968 + BT0114 0.42 -1.6 105,971 + BT0114 0.42 -1.0 106,017 - BT0114 0.47 -2.8 106,018 + BT0114 0.47 -2.2 106,094 + BT0114 0.54 -2.6 106,177 + BT0114 0.62 -0.0 106,216 - BT0114 0.66 -0.0 106,268 + BT0114 0.71 -1.0 106,269 - BT0114 0.71 -2.8 106,269 - BT0114 0.71 -0.8 106,272 + BT0114 0.71 +0.3 106,272 + BT0114 0.71 +0.5 106,272 + BT0114 0.71 -1.6 106,273 - BT0114 0.71 -4.0 106,395 + BT0114 0.83 -0.0 106,427 + BT0114 0.86 -1.6 106,427 + BT0114 0.86 -1.6 106,482 + -0.0 106,483 - -0.2 106,483 - -1.6 106,511 - -0.0 106,529 - -1.0 106,537 - -0.0 106,640 + BT0115 0.13 -0.3 106,640 + BT0115 0.13 -1.5 106,747 + BT0115 0.38 -1.0 106,794 + BT0115 0.48 -0.0 106,815 + BT0115 0.53 -2.7 106,877 + BT0115 0.68 +0.4 106,987 + -0.0 107,020 + -1.6 107,020 + -0.0 107,154 + -0.0 107,277 + BT0116 0.15 -0.0 107,332 - BT0116 0.18 -1.4 107,494 - BT0116 0.28 -2.8 107,498 - BT0116 0.28 -1.0
Or see this region's nucleotide sequence