Experiment: Mannan from Saccharomyces cerevisiae; filtered 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4581 and BT4582 are separated by 164 nucleotides BT4582 and BT4583 are separated by 119 nucleotides
BT4581: BT4581 - alpha-glucosidase (NCBI ptt file), at 6,001,250 to 6,003,409
BT4581
BT4582: BT4582 - amidophosphoribosyltransferase precursor (NCBI ptt file), at 6,003,574 to 6,004,983
BT4582
BT4583: BT4583 - peptidase T (tripeptide aminopeptidase) (NCBI ptt file), at 6,005,103 to 6,006,326
BT4583
Position (kb)
6003
6004
6005 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6002.582 kb on + strand at 6002.585 kb on - strand, within BT4581 at 6002.667 kb on + strand, within BT4581 at 6002.667 kb on + strand, within BT4581 at 6002.668 kb on - strand, within BT4581 at 6002.668 kb on - strand, within BT4581 at 6002.668 kb on - strand, within BT4581 at 6002.724 kb on + strand, within BT4581 at 6002.747 kb on - strand, within BT4581 at 6002.813 kb on - strand, within BT4581 at 6002.825 kb on - strand, within BT4581 at 6002.872 kb on + strand, within BT4581 at 6002.872 kb on + strand, within BT4581 at 6002.929 kb on - strand, within BT4581 at 6002.936 kb on - strand, within BT4581 at 6002.965 kb on - strand, within BT4581 at 6003.012 kb on - strand, within BT4581 at 6003.078 kb on - strand, within BT4581 at 6003.101 kb on + strand, within BT4581 at 6003.106 kb on + strand, within BT4581 at 6003.218 kb on + strand at 6003.230 kb on + strand at 6003.233 kb on + strand at 6003.234 kb on - strand at 6003.234 kb on - strand at 6003.623 kb on + strand at 6003.624 kb on - strand at 6003.628 kb on + strand at 6003.683 kb on - strand at 6003.683 kb on - strand at 6003.729 kb on + strand, within BT4582 at 6003.934 kb on - strand, within BT4582 at 6003.976 kb on - strand, within BT4582 at 6003.980 kb on + strand, within BT4582 at 6003.981 kb on - strand, within BT4582 at 6003.981 kb on - strand, within BT4582 at 6004.026 kb on + strand, within BT4582 at 6004.211 kb on + strand, within BT4582 at 6004.211 kb on + strand, within BT4582 at 6004.212 kb on - strand, within BT4582 at 6004.291 kb on + strand, within BT4582 at 6004.291 kb on + strand, within BT4582 at 6004.292 kb on - strand, within BT4582 at 6004.292 kb on - strand, within BT4582 at 6004.315 kb on + strand, within BT4582 at 6004.316 kb on - strand, within BT4582 at 6004.332 kb on - strand, within BT4582 at 6004.426 kb on - strand, within BT4582 at 6004.426 kb on - strand, within BT4582 at 6004.449 kb on + strand, within BT4582 at 6004.462 kb on + strand, within BT4582 at 6004.463 kb on - strand, within BT4582 at 6004.504 kb on - strand, within BT4582 at 6004.504 kb on - strand, within BT4582 at 6004.676 kb on + strand, within BT4582 at 6004.677 kb on - strand, within BT4582 at 6004.680 kb on + strand, within BT4582 at 6004.680 kb on + strand, within BT4582 at 6004.681 kb on - strand, within BT4582 at 6004.708 kb on + strand, within BT4582 at 6004.708 kb on + strand, within BT4582 at 6004.740 kb on + strand, within BT4582 at 6004.770 kb on + strand, within BT4582 at 6004.774 kb on - strand, within BT4582 at 6004.774 kb on - strand, within BT4582 at 6004.968 kb on + strand at 6004.969 kb on - strand at 6004.981 kb on + strand at 6004.981 kb on + strand at 6004.988 kb on + strand at 6004.991 kb on - strand at 6005.056 kb on + strand at 6005.102 kb on + strand at 6005.130 kb on + strand at 6005.209 kb on - strand at 6005.245 kb on + strand, within BT4583 at 6005.295 kb on - strand, within BT4583 at 6005.296 kb on + strand, within BT4583 at 6005.296 kb on + strand, within BT4583 at 6005.297 kb on - strand, within BT4583 at 6005.297 kb on - strand, within BT4583 at 6005.368 kb on + strand, within BT4583 at 6005.378 kb on + strand, within BT4583 at 6005.391 kb on + strand, within BT4583 at 6005.392 kb on - strand, within BT4583 at 6005.392 kb on - strand, within BT4583 at 6005.406 kb on - strand, within BT4583 at 6005.471 kb on + strand, within BT4583 at 6005.471 kb on + strand, within BT4583 at 6005.526 kb on - strand, within BT4583 at 6005.766 kb on - strand, within BT4583 at 6005.766 kb on - strand, within BT4583 at 6005.793 kb on - strand, within BT4583 at 6005.801 kb on - strand, within BT4583 at 6005.875 kb on - strand, within BT4583 at 6005.934 kb on + strand, within BT4583 at 6005.935 kb on - strand, within BT4583 at 6005.935 kb on - strand, within BT4583 at 6005.936 kb on + strand, within BT4583 at 6005.936 kb on + strand, within BT4583
Per-strain Table
Position Strand Gene LocusTag Fraction Mannan from Saccharomyces cerevisiae; filtered 2.5 mg/ml (C) remove 6,002,582 + -0.1 6,002,585 - BT4581 0.62 -0.1 6,002,667 + BT4581 0.66 -1.9 6,002,667 + BT4581 0.66 -2.1 6,002,668 - BT4581 0.66 +0.2 6,002,668 - BT4581 0.66 -2.7 6,002,668 - BT4581 0.66 -0.8 6,002,724 + BT4581 0.68 -2.4 6,002,747 - BT4581 0.69 -0.1 6,002,813 - BT4581 0.72 -1.3 6,002,825 - BT4581 0.73 -2.7 6,002,872 + BT4581 0.75 -2.2 6,002,872 + BT4581 0.75 -0.4 6,002,929 - BT4581 0.78 -1.1 6,002,936 - BT4581 0.78 +0.9 6,002,965 - BT4581 0.79 -2.1 6,003,012 - BT4581 0.82 -2.7 6,003,078 - BT4581 0.85 -1.1 6,003,101 + BT4581 0.86 +1.1 6,003,106 + BT4581 0.86 +1.2 6,003,218 + -0.1 6,003,230 + -2.1 6,003,233 + -0.1 6,003,234 - -0.1 6,003,234 - +1.1 6,003,623 + -1.9 6,003,624 - -2.1 6,003,628 + -0.1 6,003,683 - -0.1 6,003,683 - -2.2 6,003,729 + BT4582 0.11 +0.8 6,003,934 - BT4582 0.26 -1.4 6,003,976 - BT4582 0.29 -1.4 6,003,980 + BT4582 0.29 +1.5 6,003,981 - BT4582 0.29 +1.5 6,003,981 - BT4582 0.29 +0.5 6,004,026 + BT4582 0.32 -0.9 6,004,211 + BT4582 0.45 +1.9 6,004,211 + BT4582 0.45 -0.1 6,004,212 - BT4582 0.45 -0.1 6,004,291 + BT4582 0.51 +0.9 6,004,291 + BT4582 0.51 -0.1 6,004,292 - BT4582 0.51 -2.4 6,004,292 - BT4582 0.51 +2.6 6,004,315 + BT4582 0.53 -0.1 6,004,316 - BT4582 0.53 +1.5 6,004,332 - BT4582 0.54 -0.1 6,004,426 - BT4582 0.60 -1.1 6,004,426 - BT4582 0.60 +0.3 6,004,449 + BT4582 0.62 +0.9 6,004,462 + BT4582 0.63 +0.2 6,004,463 - BT4582 0.63 -0.1 6,004,504 - BT4582 0.66 -0.7 6,004,504 - BT4582 0.66 -2.1 6,004,676 + BT4582 0.78 +3.5 6,004,677 - BT4582 0.78 +0.7 6,004,680 + BT4582 0.78 +1.1 6,004,680 + BT4582 0.78 +0.5 6,004,681 - BT4582 0.79 -2.7 6,004,708 + BT4582 0.80 +0.6 6,004,708 + BT4582 0.80 -0.1 6,004,740 + BT4582 0.83 -0.7 6,004,770 + BT4582 0.85 -1.1 6,004,774 - BT4582 0.85 +0.6 6,004,774 - BT4582 0.85 -1.1 6,004,968 + +1.5 6,004,969 - -0.1 6,004,981 + +1.9 6,004,981 + -1.1 6,004,988 + -1.7 6,004,991 - -0.8 6,005,056 + -2.1 6,005,102 + -2.3 6,005,130 + -1.9 6,005,209 - +0.9 6,005,245 + BT4583 0.12 -1.4 6,005,295 - BT4583 0.16 -1.4 6,005,296 + BT4583 0.16 +0.5 6,005,296 + BT4583 0.16 +0.7 6,005,297 - BT4583 0.16 +0.4 6,005,297 - BT4583 0.16 -0.1 6,005,368 + BT4583 0.22 -0.7 6,005,378 + BT4583 0.22 -0.4 6,005,391 + BT4583 0.24 -2.4 6,005,392 - BT4583 0.24 -1.8 6,005,392 - BT4583 0.24 -2.4 6,005,406 - BT4583 0.25 -1.1 6,005,471 + BT4583 0.30 -0.1 6,005,471 + BT4583 0.30 -1.9 6,005,526 - BT4583 0.35 -2.6 6,005,766 - BT4583 0.54 -1.7 6,005,766 - BT4583 0.54 -0.1 6,005,793 - BT4583 0.56 +0.3 6,005,801 - BT4583 0.57 -1.7 6,005,875 - BT4583 0.63 -0.1 6,005,934 + BT4583 0.68 -2.1 6,005,935 - BT4583 0.68 +0.3 6,005,935 - BT4583 0.68 -1.7 6,005,936 + BT4583 0.68 -2.4 6,005,936 + BT4583 0.68 -0.1
Or see this region's nucleotide sequence