Experiment: Mannan from Saccharomyces cerevisiae; filtered 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1145 and BT1146 are separated by 48 nucleotides BT1146 and BT1147 are separated by 122 nucleotides BT1147 and BT1148 are separated by 202 nucleotides BT1148 and BT1149 are separated by 42 nucleotides
BT1145: BT1145 - glucose-inhibited division protein (NCBI ptt file), at 1,428,568 to 1,429,188
BT1145
BT1146: BT1146 - putative metallo-beta-lactamase (NCBI ptt file), at 1,429,237 to 1,429,848
BT1146
BT1147: BT1147 - glycine dehydrogenase [decarboxylating] (NCBI ptt file), at 1,429,971 to 1,432,820
BT1147
BT1148: BT1148 - putative NADH dehydrogenase/NAD(P)H nitroreductase (NCBI ptt file), at 1,433,023 to 1,433,592
BT1148
BT1149: BT1149 - Type II restriction enzyme HpaII (NCBI ptt file), at 1,433,635 to 1,434,720
BT1149
Position (kb)
1429
1430
1431
1432
1433 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1429.041 kb on + strand, within BT1145 at 1429.170 kb on - strand at 1429.255 kb on + strand at 1429.332 kb on - strand, within BT1146 at 1429.358 kb on + strand, within BT1146 at 1429.381 kb on - strand, within BT1146 at 1429.500 kb on + strand, within BT1146 at 1429.547 kb on - strand, within BT1146 at 1429.547 kb on - strand, within BT1146 at 1429.637 kb on - strand, within BT1146 at 1429.781 kb on + strand, within BT1146 at 1429.782 kb on - strand, within BT1146 at 1429.871 kb on + strand at 1429.875 kb on - strand at 1429.906 kb on + strand at 1429.907 kb on - strand at 1429.910 kb on + strand at 1429.932 kb on + strand at 1429.936 kb on + strand at 1429.992 kb on - strand at 1430.139 kb on + strand at 1430.197 kb on - strand at 1430.214 kb on + strand at 1430.219 kb on + strand at 1430.272 kb on - strand, within BT1147 at 1430.273 kb on + strand, within BT1147 at 1430.274 kb on - strand, within BT1147 at 1430.274 kb on - strand, within BT1147 at 1430.280 kb on + strand, within BT1147 at 1430.474 kb on - strand, within BT1147 at 1430.644 kb on - strand, within BT1147 at 1430.846 kb on + strand, within BT1147 at 1430.847 kb on - strand, within BT1147 at 1430.930 kb on + strand, within BT1147 at 1431.008 kb on - strand, within BT1147 at 1431.008 kb on - strand, within BT1147 at 1431.010 kb on - strand, within BT1147 at 1431.053 kb on + strand, within BT1147 at 1431.054 kb on - strand, within BT1147 at 1431.210 kb on + strand, within BT1147 at 1431.226 kb on + strand at 1431.227 kb on - strand, within BT1147 at 1431.264 kb on - strand, within BT1147 at 1431.430 kb on - strand, within BT1147 at 1431.532 kb on + strand, within BT1147 at 1431.533 kb on - strand, within BT1147 at 1431.714 kb on + strand, within BT1147 at 1431.932 kb on + strand, within BT1147 at 1431.933 kb on - strand, within BT1147 at 1431.994 kb on - strand, within BT1147 at 1431.995 kb on + strand, within BT1147 at 1431.996 kb on - strand, within BT1147 at 1431.996 kb on - strand, within BT1147 at 1432.136 kb on - strand, within BT1147 at 1432.159 kb on + strand, within BT1147 at 1432.226 kb on + strand, within BT1147 at 1432.242 kb on + strand, within BT1147 at 1432.409 kb on - strand, within BT1147 at 1432.521 kb on - strand, within BT1147 at 1432.576 kb on + strand at 1432.577 kb on - strand at 1432.736 kb on + strand at 1432.736 kb on + strand at 1432.819 kb on - strand at 1432.846 kb on - strand at 1432.892 kb on + strand at 1432.915 kb on - strand at 1432.944 kb on + strand at 1433.014 kb on - strand at 1433.024 kb on + strand at 1433.054 kb on - strand at 1433.069 kb on - strand at 1433.096 kb on - strand, within BT1148 at 1433.111 kb on + strand, within BT1148 at 1433.111 kb on + strand, within BT1148 at 1433.113 kb on + strand, within BT1148 at 1433.113 kb on + strand, within BT1148 at 1433.119 kb on - strand, within BT1148 at 1433.325 kb on - strand, within BT1148 at 1433.422 kb on - strand, within BT1148 at 1433.538 kb on - strand at 1433.566 kb on - strand at 1433.595 kb on - strand at 1433.603 kb on + strand at 1433.612 kb on + strand at 1433.613 kb on - strand at 1433.678 kb on + strand at 1433.678 kb on + strand at 1433.679 kb on - strand at 1433.679 kb on - strand at 1433.679 kb on - strand at 1433.679 kb on - strand at 1433.708 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mannan from Saccharomyces cerevisiae; filtered 2.5 mg/ml (C) remove 1,429,041 + BT1145 0.76 -1.4 1,429,170 - +0.5 1,429,255 + +1.7 1,429,332 - BT1146 0.16 +2.7 1,429,358 + BT1146 0.20 +0.2 1,429,381 - BT1146 0.24 +0.7 1,429,500 + BT1146 0.43 +1.3 1,429,547 - BT1146 0.51 +0.5 1,429,547 - BT1146 0.51 -1.1 1,429,637 - BT1146 0.65 -1.1 1,429,781 + BT1146 0.89 +2.5 1,429,782 - BT1146 0.89 +2.1 1,429,871 + +0.9 1,429,875 - +0.3 1,429,906 + -1.7 1,429,907 - -0.1 1,429,910 + +0.8 1,429,932 + -1.7 1,429,936 + -0.1 1,429,992 - +0.5 1,430,139 + -1.7 1,430,197 - -2.1 1,430,214 + -1.7 1,430,219 + -1.1 1,430,272 - BT1147 0.11 -1.7 1,430,273 + BT1147 0.11 -1.4 1,430,274 - BT1147 0.11 +1.7 1,430,274 - BT1147 0.11 +1.5 1,430,280 + BT1147 0.11 +0.9 1,430,474 - BT1147 0.18 -1.3 1,430,644 - BT1147 0.24 +0.3 1,430,846 + BT1147 0.31 -0.1 1,430,847 - BT1147 0.31 -2.4 1,430,930 + BT1147 0.34 +1.9 1,431,008 - BT1147 0.36 -2.5 1,431,008 - BT1147 0.36 -1.4 1,431,010 - BT1147 0.36 -0.1 1,431,053 + BT1147 0.38 -4.1 1,431,054 - BT1147 0.38 -0.1 1,431,210 + BT1147 0.43 +0.1 1,431,226 + -0.1 1,431,227 - BT1147 0.44 -0.8 1,431,264 - BT1147 0.45 +0.9 1,431,430 - BT1147 0.51 -0.1 1,431,532 + BT1147 0.55 -1.7 1,431,533 - BT1147 0.55 -0.1 1,431,714 + BT1147 0.61 -0.1 1,431,932 + BT1147 0.69 +2.2 1,431,933 - BT1147 0.69 +0.6 1,431,994 - BT1147 0.71 -0.1 1,431,995 + BT1147 0.71 -2.6 1,431,996 - BT1147 0.71 -0.1 1,431,996 - BT1147 0.71 +0.9 1,432,136 - BT1147 0.76 +2.5 1,432,159 + BT1147 0.77 -0.1 1,432,226 + BT1147 0.79 -0.1 1,432,242 + BT1147 0.80 -0.9 1,432,409 - BT1147 0.86 -0.5 1,432,521 - BT1147 0.89 -0.7 1,432,576 + -0.1 1,432,577 - -1.7 1,432,736 + +0.5 1,432,736 + -0.1 1,432,819 - -1.1 1,432,846 - -2.0 1,432,892 + -1.1 1,432,915 - +1.9 1,432,944 + -1.7 1,433,014 - -2.5 1,433,024 + -0.4 1,433,054 - -0.7 1,433,069 - -0.4 1,433,096 - BT1148 0.13 -0.8 1,433,111 + BT1148 0.15 -2.1 1,433,111 + BT1148 0.15 -0.1 1,433,113 + BT1148 0.16 -1.1 1,433,113 + BT1148 0.16 -2.7 1,433,119 - BT1148 0.17 -0.8 1,433,325 - BT1148 0.53 -1.1 1,433,422 - BT1148 0.70 -0.1 1,433,538 - -0.8 1,433,566 - -1.4 1,433,595 - -0.5 1,433,603 + +1.9 1,433,612 + -0.1 1,433,613 - -2.4 1,433,678 + +0.5 1,433,678 + -1.7 1,433,679 - +0.5 1,433,679 - -2.4 1,433,679 - -1.5 1,433,679 - -2.5 1,433,708 + -0.1
Or see this region's nucleotide sequence